LOCUS       sequence-437204-       12515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TALEcharm Kv2 expression in mammalian cells; targets Prnp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Neumann EN, Bertozzi TM, Wu E, Serack F, Harvey JW, Brauer PP, 
            Pirtle CP, Coffey A, Howard M, Kamath N, Lenz K, Guzman K, Raymond 
            MH, Khalil AS, Deverman BE, Minikel EV, Vallabh SM, Weissman JS
  TITLE     Brainwide silencing of prion protein by AAV-mediated delivery of an 
            engineered compact epigenetic editor.
  JOURNAL   Science. 2024 Jun 28;384(6703):ado7082. doi: 
            10.1126/science.ado7082. Epub 2024 Jun 28.
  PUBMED    38935715
REFERENCE   2  (bases 1 to 12515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-28"; volume: "384(6703):ado7082. doi"; pages: " 
            10.1126/science.ado7082. Epub 2024 Jun 28"
COMMENT     Sequence Label: pENN491_TALEcharm_MmT8x
FEATURES             Location/Qualifiers
     source          1..12515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(114..133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      927..1515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1416..1435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1669..1686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1803..1824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1839..1844,1845..1862,1863..1869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1877..1893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1882..1904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1901..1917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1901..1917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1936..1956
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1938..1956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1984..2210
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2211..2391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2438..2563
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3056..3289
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3474..3518
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3749..3959
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3771..3967
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3818..3953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3999..4601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3999..4018)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4495..4515
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4644..4761
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4899..6077
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5130..6068
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6025..6045
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6085..6094
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9562..9582
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9583..9639
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    10351..10939
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10404..10424)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10822..10833)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11011..11244
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11322..11443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11359..11378)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11413..11432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11483..11618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11638..11657)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11639..11657)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11667..11684)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11667..11683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11676..11698)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11825..12280
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11912..11931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12122..12143
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12306..12410
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12411..12479,12480..12515,1..756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
       61 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     1801 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     1861 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     1921 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     1981 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     2041 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     2101 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     2161 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     2221 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     2281 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     2341 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     2401 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     2461 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     2521 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     2581 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     2641 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     2701 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     2761 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     2821 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     2881 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     2941 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     3001 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     3061 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     3121 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     3181 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     3241 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     3301 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     3361 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     3421 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     3481 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     3541 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3601 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3661 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac
     3721 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa
     3781 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3841 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3901 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3961 ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg
     4021 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4081 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4141 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     4201 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     4261 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     4321 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     4381 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     4441 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     4501 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     4561 cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga
     4621 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     4681 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     4741 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     4801 cggctcgagg cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca
     4861 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     4921 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     4981 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5041 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5101 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     5161 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     5221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     5281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     5341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     5401 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     5461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     5521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     5581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     5641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     5701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     5761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     5821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     5881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     5941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     6001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     6061 catttcaggt gtcgtgaggg atccgccacc atggccagga ccaaacaaac tgccagaaag
     6121 agcaccgggg ggaaagcgcc ccgaaaacag ctggcgacca aagcagcccg caaaagcgca
     6181 ccgggaggtg caagcagtgg ggcaggaagt tcctcaggag gaagtgcggc gggatccgga
     6241 agcggtggcg catcaagtgg cgccgcaagt agcagtgccg ggtccgcagc tggctccggg
     6301 tcctcccctg ttgagatcta caagaccgtg tccgcctgga agcggcagcc aatcagagtg
     6361 ctgagcctgt tcggcaatat tgacaaggaa ctgaagtctc tgggcttcct ggaaagcggc
     6421 agccacagcg agggcggcac actgaagtac gtggaagatg tgaccaacgt ggtgcggaga
     6481 gatgtggaaa agtggggacc tttcgacctg ctgtacggca gcacccagcc tctgggcgga
     6541 agctgcgaca ggtgtcctgg ctggtacatg ttccaatttc atagaatcct gcagtacgcc
     6601 agacctagac aggagtctca gcccttcttc tggatcttca tggataacct gctgctcaca
     6661 gaggacgacc agatgaccac cgtgcggttc ctgcaaacag aggccgtcac cctgcgcgac
     6721 gtgagatcta gagtgctgca gaacgccgtc cgggtgtgga gcaacatccc cggtcttaaa
     6781 agcaagcacg aggctctgtc cccaaaagag gaagagagcc tgcagggcca cgtgcggacc
     6841 agatctaagc tggccgctcc taaagtggac cccctggtga agaactgcct gctgcctctg
     6901 agagagtact ttaagtattt cagccagagc agcctgcccc tgggcggccc ttctagcggt
     6961 gctccacctc caagcggcgg ctcgccagcc ggcagcccta ccagcaccga ggagggcaca
     7021 agcgagagcg caacacctga gagcggcccc ggcacaagca ccgagccttc tgaaggaagc
     7081 gcccctggga gccctgccgg cagccccacc agcacagaag agggcacctc taccgagccc
     7141 agcgagggta gcgcccctgg cacaagcaca gaaccctctg agaagcggac tgccgatgga
     7201 tctgagtttg agagccctaa gaaaaaaaaa aaggttggca ttcaccgcgg ggtacctatg
     7261 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc
     7321 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat
     7381 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat
     7441 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg
     7501 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg
     7561 ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta
     7621 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct tacgccagac
     7681 caggtcgttg ccatcgcaag ccacgacgga ggaaaacagg ctctggagac agtccagaga
     7741 cttcttccgg tgttgtgcca ggaccacggt ttttctcagg ccgacatcgt gaaaatcgcc
     7801 ggtcatgatg gtggcgcaca ggccctacag gcggtactgg aattggaacc caccctgcgc
     7861 gaaaggggac tcacaccaca acaggtggtc gccatagcta atggcggagg aaagcaggct
     7921 cttgagactg tgcagcgcct gctcccagtt ctgtgtcagg ctcatggcct caccccagat
     7981 caggtggttg ccatcgcttc taaccacggg ggcaaacaag ccctggagac tgtccaacgg
     8041 ctgctcccgg ttttgtgcca ggatcacggc cttaccccag cacaggtcgt tgccattgca
     8101 tccaatcatg gcggtaaaca ggctctggaa actgtccaag cgttgctgcc tgtactctgt
     8161 caggaccacg gattgacccc tgaccaagtc gttgcaatcg caaatcacga cggcgggaag
     8221 caggctctgg aaactgttca aagactcctt cccgttctct gtcaggcaca cggactgacc
     8281 ccagaacagg tcgtggcaat cgcttcaaat ggcggaggca aacaggctct cgaaacagtg
     8341 cagaggctcc ttccggtcct gtgtcaggcc cacggattct cacaggcaga catcgtgaag
     8401 attgctggcc acgatggtgg cgcgcaagca ctccaagcgg tgttggaatt ggagcccact
     8461 ctccgggaaa gggggctcac gcccgatcaa gtagtggcaa ttgcaagtca cgacggagga
     8521 aaacaagctc ttgaaaccgt gcaaagactc cttccggtgt tgtgtcaaga tcatggcctg
     8581 acgccggccc aagttgtggc cattgcttct catgacgggg gtaagcaagc ccttgaaact
     8641 gtccaggcct tgctgcccgt gctgtgtcaa gctcacggac tcacaccaga ccaagtggtt
     8701 gccattgcaa atcacgatgg aggaaaacaa gcgttggaga ctgttcaacg attgcttcca
     8761 gtgctttgtc aagcccatgg tctgacacct gatcaagttg ttgctatcgc aaatggcgga
     8821 ggtaaacaag ctttagaaac agtacaacgg ttgctacctg ttctttgcca agctcacggt
     8881 ctgacgcccg agcaggtagt agccatagct tctaatcacg gcggaaagca agcactggag
     8941 accgtccagc gcctccttcc tgtactctgc caagcgcacg ggttgacgcc tcaacaagta
     9001 gtggcaatcg cgaacggggg aggcaaacag gctctggaga cagttcaaag gcttctccca
     9061 gtgttgtgtc aagcacacgg ttactccaga gcggatattg tcaaaattgc cggaaatcac
     9121 ggtggggcgc aggcccttaa agccgtagtg atgcacggac ctacgctttg cgaacgcgga
     9181 ctcacgccgg atcaggttgt tgccatagct aataacggag gggggaaaca agctttggaa
     9241 acagttcaaa gacttcttcc tgtgctctgt caggaccacg ggcttacgcc acaacaggtg
     9301 gtggcgattg catctaatgg aggcggacgc cctgccttgg agtccatcgt agcccaattg
     9361 tccaggcccg atcccgcgtt ggctgcgtta acgaatgacc atctggtggc gttggcatgt
     9421 cttggtggac gacccgcgct cgatgcagtc aaaaagggtc tgcctcatgc tcccgcattg
     9481 atcaaaagaa ccaaccggcg gattcccgag agaacttccc atcgagtcgc gaagaggacc
     9541 gccgacggca gcgagttcga gcccaagaaa aagcggaagg tggctacaaa cttcagcctg
     9601 ctgaaacagg ccggcgacgt cgaggaaaat cctggcccca gcgagctgat taaggaaaac
     9661 atgcacatga agctgtatat ggaaggcacc gtggacaacc accatttcaa gtgcaccagc
     9721 gagggcgaag gcaagcccta cgagggaaca cagaccatgc ggatcaaggt ggtggaaggc
     9781 ggccctctgc cattcgcctt cgacatcctc gccacctctt tcctgtacgg cagtaagacc
     9841 tttatcaacc acacccaggg catccccgat ttcttcaagc agagcttccc cgagggattt
     9901 acctgggaga gagtgaccac ctacgaggac ggcggcgtgc tgacagccac ccaggacacc
     9961 agcctgcagg acggctgtct gatctacaac gtgaagatca gaggcgtgaa cttcaccagc
    10021 aacggccctg tgatgcagaa aaaaaccctg ggctgggagg cctttacaga gacactgtac
    10081 cccgctgatg gaggcctgga aggtagaaac gacatggccc tgaaactggt cggcggcagc
    10141 cacctgattg ccaatatcaa gaccacatac cggagcaaga aaccagctaa aaacctgaag
    10201 atgcctggcg tttactacgt ggattacaga ctggagcgga tcaaggaagc caacaacgag
    10261 acatatgtgg aacagcacga ggtggccgta gctagatact gcgacctgcc ttctaagctt
    10321 ggccacaagc tgaactaagc tagcgtcgac aatcaacctc tggattacaa aatttgtgaa
    10381 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
    10441 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
    10501 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
    10561 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
    10621 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    10681 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    10741 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    10801 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    10861 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    10921 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
    10981 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    11041 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    11101 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    11161 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    11221 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
    11281 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
    11341 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11401 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
    11461 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    11521 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    11581 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
    11641 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
    11701 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    11761 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    11821 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    11881 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    11941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    12001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    12061 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    12121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    12181 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    12241 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    12301 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    12361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    12421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    12481 acccagaaac gctggtgaaa gtaaaagatg ctgaa
//
