LOCUS       sequence-437205-       12070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mini CRISPRcharm expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Neumann EN, Bertozzi TM, Wu E, Serack F, Harvey JW, Brauer PP, 
            Pirtle CP, Coffey A, Howard M, Kamath N, Lenz K, Guzman K, Raymond 
            MH, Khalil AS, Deverman BE, Minikel EV, Vallabh SM, Weissman JS
  TITLE     Brainwide silencing of prion protein by AAV-mediated delivery of an 
            engineered compact epigenetic editor.
  JOURNAL   Science. 2024 Jun 28;384(6703):ado7082. doi: 
            10.1126/science.ado7082. Epub 2024 Jun 28.
  PUBMED    38935715
REFERENCE   2  (bases 1 to 12070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-28"; volume: "384(6703):ado7082. doi"; pages: " 
            10.1126/science.ado7082. Epub 2024 Jun 28"
COMMENT     Sequence Label: pENN496_miniCRISPRcharm_dSaCas9
FEATURES             Location/Qualifiers
     source          1..12070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(114..133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      927..1515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1416..1435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1669..1686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1803..1824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1839..1844,1845..1862,1863..1869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1877..1893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1882..1904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1901..1917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1901..1917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1936..1956
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1938..1956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1984..2210
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2211..2391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2438..2563
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3056..3289
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3474..3518
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3749..3959
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3771..3967
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3818..3953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3999..4601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3999..4018)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4495..4515
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4644..4761
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4899..5110
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5118..5127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6216..6236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9417..9437
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9438..9494
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    10205..10253
                     /note="synthetic polyadenylation signal"
     primer_bind     10268..10288
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10401..10476
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     LTR             10566..10799
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10877..10998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10914..10933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10968..10987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11038..11173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11193..11212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11194..11212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11222..11239)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11222..11238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11231..11253)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11380..11835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11467..11486)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11677..11698
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11861..11965
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11966..12034,12035..12070,1..756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
       61 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     1801 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     1861 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     1921 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     1981 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     2041 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     2101 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     2161 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     2221 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     2281 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     2341 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     2401 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     2461 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     2521 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     2581 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     2641 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     2701 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     2761 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     2821 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     2881 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     2941 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     3001 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     3061 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     3121 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     3181 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     3241 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     3301 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     3361 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     3421 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     3481 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     3541 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3601 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3661 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac
     3721 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa
     3781 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3841 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3901 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3961 ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg
     4021 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4081 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4141 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     4201 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     4261 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     4321 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     4381 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     4441 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     4501 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     4561 cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga
     4621 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     4681 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     4741 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     4801 cggctcgagg cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca
     4861 gctaatggac cttctaggtc ttgaaaggag tggcggccgg gcagagcgca catcgcccac
     4921 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     4981 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     5041 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     5101 cagaacacag cggatccgcc accatggcca ggaccaaaca aactgccaga aagagcaccg
     5161 gggggaaagc gccccgaaaa cagctggcga ccaaagcagc ccgcaaaagc gcaccgggag
     5221 gtgcaagcag tggggcagga agttcctcag gaggaagtgc ggcgggaagc ggttccggtg
     5281 gcgcatcaag tggcgccgca agtagcagtg ccgggtccgc agctggctcc gggtcctccc
     5341 ctgttgagat ctacaagacc gtgtccgcct ggaagcggca gccaatcaga gtgctgagcc
     5401 tgttcggcaa tattgacaag gaactgaagt ctctgggctt cctggaaagc ggcagccaca
     5461 gcgagggcgg cacactgaag tacgtggaag atgtgaccaa cgtggtgcgg agagatgtgg
     5521 aaaagtgggg acctttcgac ctgctgtacg gcagcaccca gcctctgggc ggaagctgcg
     5581 acaggtgtcc tggctggtac atgttccaat ttcatagaat cctgcagtac gccagaccta
     5641 gacaggagtc tcagcccttc ttctggatct tcatggataa cctgctgctc acagaggacg
     5701 accagatgac caccgtgcgg ttcctgcaaa cagaggccgt caccctgcgc gacgtgagat
     5761 ctagggtgct gcagaacgcc gtccgggtgt ggagcaacat ccccggtctt aaaagcaagc
     5821 acgaggctct gtccccaaaa gaggaagaga gcctgcaggg ccacgtgcgg accagatcta
     5881 agctggccgc tcctaaagtg gaccccctgg tgaagaactg cctgctgcct ctgagagagt
     5941 actttaagta tttcagccag agcagcctgc ccctgggagg acctagcagc ggcgccccac
     6001 ctccaagcgg aggctctcca gctggcagcc ctaccagcac agaggaagga acaagcgaga
     6061 gcgccacacc tgagagcgga cctggcacct ctaccgagcc tagtgaggga tctgctcctg
     6121 gcagccctgc tggaagtcca acctctacag aagaaggcac cagcacagag ccttctgaag
     6181 gctctgcccc tggaacatcc accgagccta gcgagccaaa gaagaagcgg aaggtcggta
     6241 tccacggagt cccagcagcc aaaagaaact atatcctggg acttgcgatc ggtatcactt
     6301 ctgtcggcta cggtataatt gactacgaga cacgcgatgt tatcgatgcc ggagtcagac
     6361 tgttcaaaga ggcgaatgtc gaaaacaatg aaggcaggag gtctaagcgg ggagctcgaa
     6421 gattgaaacg ccggagacgc catagaatcc aacgcgttaa gaaactcctt tttgactata
     6481 accttctgac cgatcactct gagctgagtg gcataaaccc ttatgaggca cgagtcaaag
     6541 gcttgtccca gaagctgtcc gaggaagagt tctcagcggc tctcctccat ctcgccaaac
     6601 ggcggggggt ccataatgtt aacgaggtcg aagaggacac tgggaatgaa cttagtacta
     6661 aagagcaaat atctcgcaat tcaaaggcac ttgaggaaaa gtacgttgcc gaactgcagt
     6721 tggagcgact caaaaaggac ggagaagttc gcggttccat taatagattt aaaacgtccg
     6781 actatgtgaa agaagccaag caactgctga aagttcaaaa agcctatcac cagctggacc
     6841 agtccttcat cgatacgtac atcgatctcc ttgagacgcg aagaacttac tacgaagggc
     6901 ccggcgaggg gagccccttc gggtggaagg atataaaaga atggtatgaa atgcttatgg
     6961 gccactgcac gtacttcccg gaagaactcc ggagcgtgaa atacgcttat aatgccgatc
     7021 tttacaatgc cttgaatgac ctgaataacc tggtcattac acgggatgaa aatgagaaac
     7081 tggagtatta cgaaaaattt cagattatag aaaatgtttt caaacaaaag aagaagccta
     7141 cgttgaaaca gatcgccaaa gaaatcctcg ttaacgagga ggatattaag gggtaccgag
     7201 tcacctcaac cggaaagcca gagtttacta atctcaaggt gtatcacgac attaaggata
     7261 tcacggccag aaaggagata atcgaaaacg ctgaactgct cgatcaaata gcaaagatac
     7321 tcacaatcta ccaatcctcc gaggacatcc aggaggagtt gactaatctg aatagcgaac
     7381 tcacacaaga ggagatagag caaatctcca atttgaaggg ctacacgggg actcataatc
     7441 tgtctcttaa agccatcaac ttgatactcg atgagttgtg gcataccaat gataaccaaa
     7501 tagcaatctt caataggttg aagctcgtac cgaagaaagt cgatctttct cagcagaagg
     7561 aaataccgac tactctggtg gacgatttca tcctttcacc ggtagttaag cgcagcttta
     7621 ttcaatccat caaagtgatt aacgctatta ttaaaaaata tggcctgcca aatgatatta
     7681 tcattgaact ggcaagggaa aaaaactcca aagatgcgca gaaaatgatt aatgagatgc
     7741 aaaaacgcaa tcgccagacc aatgaacgca tagaagagat tattagaacc accggaaagg
     7801 agaatgcgaa gtatttgata gagaagatta aattgcatga catgcaggaa ggtaagtgcc
     7861 tgtacagctt ggaggcgata cctcttgaag atctcttgaa taatccgttt aattatgagg
     7921 ttgaccacat tatacccagg agtgtttcct tcgacaatag cttcaacaat aaggttctgg
     7981 ttaagcagga agaagcttcc aaaaagggta acagaacgcc ttttcaatat ttgtcaagct
     8041 cagactcaaa aatcagctat gagacattta agaaacatat attgaacctt gccaagggga
     8101 aaggacggat aagtaagaca aagaaagagt accttttgga agaacgggat attaatcgat
     8161 tttcagtcca aaaggatttc atcaatagaa atcttgtgga caccaggtat gccacgagag
     8221 ggctcatgaa tctgttgagg tcctacttcc gagtcaataa tcttgacgtg aaagtgaaga
     8281 gcattaatgg aggatttacg tccttcctcc gacggaaatg gaagttcaaa aaggaacgaa
     8341 ataaaggtta taagcatcac gcagaggatg cccttataat agccaacgcg gactttattt
     8401 ttaaagaatg gaagaagttg gataaggcaa agaaagtgat ggaaaaccaa atgttcgaag
     8461 agaagcaggc agagagtatg ccggagatcg agacagaaca agagtataaa gaaattttta
     8521 taacccccca tcaaattaaa catatcaaag atttcaagga ttacaagtat tcccataggg
     8581 tagataaaaa gcccaatcgc gagcttataa atgataccct ctatagcacc cgcaaggacg
     8641 acaaaggtaa tactctcatc gtgaataatc tgaacggtct ctatgacaaa gacaacgata
     8701 aattgaaaaa gctgataaat aaaagtcccg agaagctcct catgtaccat catgatccgc
     8761 aaacttacca gaaactcaaa cttatcatgg agcagtacgg agatgaaaag aatccacttt
     8821 ataagtacta tgaggagacg gggaactatt tgaccaagta tagcaagaag gataacggtc
     8881 cggtaattaa gaaaatcaaa tactatggca ataagcttaa tgcccacctc gatattacgg
     8941 acgactaccc taattcccgc aataaggtgg ttaagctcag tctgaaacca tatagattcg
     9001 acgtatatct ggataacggc gtttacaaat tcgtcacggt taaaaatctc gacgttatca
     9061 aaaaggaaaa ttattatgaa gtgaatagca aatgttatga ggaagctaag aagctcaaaa
     9121 agatcagcaa tcaggccgag tttattgctt ccttctacaa taatgacctg atcaaaatta
     9181 acggtgaact ttatcgggta attggcgtga acaacgatct tcttaaccga atcgaagtca
     9241 acatgataga cattacatac cgagaatacc tggagaatat gaacgacaag aggccaccaa
     9301 ggatcataaa gaccatagca tccaaaaccc agagcattaa gaagtactcc acggatattc
     9361 tgggtaattt gtacgaggtc aaatcaaaaa agcatcctca gatcatcaag aaaggtccca
     9421 agaagaagcg gaaggtggcc acaaatttca gcctgctgaa gcaggccggc gacgtggaag
     9481 agaatcctgg ccccagcgag ctgatcaaag agaacatgca catgaagctg tacatggaag
     9541 gcaccgtgga caaccatcac ttcaagtgca ccagcgaggg cgagggcaag ccctacgagg
     9601 gcacacagac catgagaatc aaggtggtgg aaggaggccc actgcccttc gccttcgaca
     9661 tcctggccac cagctttctg tacggctcaa agaccttcat caaccacacc cagggcatcc
     9721 ctgatttctt caagcagagc ttccctgagg gatttacctg ggagagagtg acaacttatg
     9781 aagatggcgg agtgctgacc gccacacaag ataccagcct gcaggacggc tgtctgatct
     9841 acaacgtgaa gatcagaggc gtgaacttca ccagcaacgg ccctgtgatg cagaaaaaaa
     9901 ccctgggctg ggaggccttt acagagacac tgtaccccgc tgatggaggc ctggaaggta
     9961 gaaacgacat ggccctgaaa ctggtcggcg gcagccacct gattgccaat atcaagacca
    10021 cataccggag caagaaacca gctaaaaacc tgaagatgcc tggcgtttac tacgtggatt
    10081 acagactgga gcggatcaag gaagccaaca acgagacata tgtggaacag cacgaggtgg
    10141 ccgtagctag atactgcgac ctgccttcta agcttggcca caagctgaac taatagctag
    10201 ctctaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtggcggccg
    10261 cggtaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat agcttaccgt
    10321 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc
    10381 gctgtggtgc cgactgggag gttttagtac tctggaaaca gaatctacta aaacaaggca
    10441 aaatgccgtg tttatctcgt caacttgttg gcgagatttt tgcggccgcc ccgcgtcgac
    10501 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    10561 gggactggaa gggctaattc actcccaaca aagacaagat ctgctttttg cttgtactgg
    10621 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10681 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10741 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10801 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
    10861 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10921 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10981 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
    11041 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    11101 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    11161 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
    11221 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    11281 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    11341 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
    11401 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    11461 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    11521 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    11581 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    11641 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    11701 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    11761 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    11821 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    11881 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    11941 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    12001 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    12061 agatgctgaa
//
