LOCUS       sequence-437207-       11071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRcharm Kv1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Neumann EN, Bertozzi TM, Wu E, Serack F, Harvey JW, Brauer PP, 
            Pirtle CP, Coffey A, Howard M, Kamath N, Lenz K, Guzman K, Raymond 
            MH, Khalil AS, Deverman BE, Minikel EV, Vallabh SM, Weissman JS
  TITLE     Brainwide silencing of prion protein by AAV-mediated delivery of an 
            engineered compact epigenetic editor.
  JOURNAL   Science. 2024 Jun 28;384(6703):ado7082. doi: 
            10.1126/science.ado7082. Epub 2024 Jun 28.
  PUBMED    38935715
REFERENCE   2  (bases 1 to 11071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-28"; volume: "384(6703):ado7082. doi"; pages: " 
            10.1126/science.ado7082. Epub 2024 Jun 28"
COMMENT     Sequence Label: pENN239_CRISPRcharm_Kv1
FEATURES             Location/Qualifiers
     source          1..11071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..658
                     /label=chicken beta-actin promoter
     intron          659..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1599..1621
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1768..1777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2866..2886
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2887..2913,2914..2916,2917..5403,5404..5406,
                     5407..6990)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2887..6990
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6991..7011
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7060..7245
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             7246..7302
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(8090..8109)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8155..8210
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8209..8228)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8571..8587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8571..8587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8584..8606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8595..8611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8619..8625,8626..8643,8644..8649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8664..8685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8744..8939
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8790..8925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8945..9079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8982..9001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9036..9055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9147..9164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9318..9906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9398..9417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10077..10868,10869..10937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10700..10719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10938..11042)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      421 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      481 atgggggggg gggggggggg ggcgcgcgcg aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattct gcagtcgacg
     1741 gtaccgcggg cccgggatcc accggtcgcc accatggcca ggaccaaaca aactgccaga
     1801 aagagcaccg gggggaaagc gccccgaaaa cagctggcga ccaaagcagc ccgcaaaagc
     1861 gcaccgggag gtgcaagcag tggggcagga agttcctcag gaggaagtgc ggcgggatcc
     1921 ggaagcggtg gcgcatcaag tggcgccgca agtagcagtg ccgggtccgc agctggctcc
     1981 gggtcctccc ctgttgagat ctacaagacc gtgtccgcct ggaagcggca gccaatcaga
     2041 gtgctgagcc tgttcggcaa tattgacaag gaactgaagt ctctgggctt cctggaaagc
     2101 ggcagccaca gcgagggcgg cacactgaag tacgtggaag atgtgaccaa cgtggtgcgg
     2161 agagatgtgg aaaagtgggg acctttcgac ctgctgtacg gcagcaccca gcctctgggc
     2221 ggaagctgcg acaggtgtcc tggctggtac atgttccaat ttcatagaat cctgcagtac
     2281 gccagaccta gacaggagtc tcagcccttc ttctggatct tcatggataa cctgctgctc
     2341 acagaggacg accagatgac caccgtgcgg ttcctgcaaa cagaggccgt caccctgcgc
     2401 gacgtgagat ctagagtgct gcagaacgcc gtccgggtgt ggagcaacat ccccggtctt
     2461 aaaagcaagc acgaggctct gtccccaaaa gaggaagaga gcctgcaggg ccacgtgcgg
     2521 accagatcta agctggccgc tcctaaagtg gaccccctgg tgaagaactg cctgctgcct
     2581 ctgagagagt actttaagta tttcagccag agcagcctgc ccctgggcgg cccttctagc
     2641 ggtgctccac ctccaagcgg cggctcgcca gccggcagcc ctaccagcac cgaggagggc
     2701 acaagcgaga gcgcaacacc tgagagcggc cccggcacaa gcaccgagcc ttctgaagga
     2761 agcgcccctg ggagccctgc cggcagcccc accagcacag aagagggcac ctctaccgag
     2821 cccagcgagg gtagcgcccc tggcacaagc acagaaccct ctgagcctaa gaaaaagcgg
     2881 aaggtgatgg acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg
     2941 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
     3001 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
     3061 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
     3121 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
     3181 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
     3241 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
     3301 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
     3361 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
     3421 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
     3481 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
     3541 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
     3601 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
     3661 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
     3721 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
     3781 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
     3841 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
     3901 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
     3961 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
     4021 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
     4081 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
     4141 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
     4201 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
     4261 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
     4321 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
     4381 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
     4441 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
     4501 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
     4561 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
     4621 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
     4681 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
     4741 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
     4801 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
     4861 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
     4921 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
     4981 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
     5041 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
     5101 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
     5161 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
     5221 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
     5281 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
     5341 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
     5401 gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
     5461 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
     5521 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
     5581 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
     5641 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
     5701 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
     5761 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
     5821 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
     5881 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
     5941 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
     6001 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
     6061 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
     6121 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
     6181 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
     6241 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
     6301 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
     6361 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
     6421 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
     6481 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
     6541 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
     6601 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
     6661 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
     6721 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
     6781 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
     6841 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
     6901 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
     6961 cggatcgacc tgtctcagct gggaggtgac cccaagaaga agcggaaggt gtccggctct
     7021 gaaacccctg gaacctccga gagcgccaca cctgaaagca gaaccctggt gaccttcaag
     7081 gacgtgttcg tggacttcac ccgcgaggaa tggaagctcc tggacaccgc ccagcaaatc
     7141 gtgtaccgga acgtgatgct ggagaattac aagaacctgg tgtctctggg ctaccagctg
     7201 acgaagcctg acgtcatcct gagactggaa aagggagagg agcctgccac aaatttcagc
     7261 ctgctgaagc aggccggcga cgtggaagag aatcctggcc ccagcgagct gatcaaagag
     7321 aacatgcaca tgaagctgta catggaaggc accgtggaca accatcactt caagtgcacc
     7381 agcgagggcg agggcaagcc ctacgagggc acacagacca tgagaatcaa ggtggtggaa
     7441 ggaggcccac tgcccttcgc cttcgacatc ctggccacca gctttctgta cggctcaaag
     7501 accttcatca accacaccca gggcatccct gatttcttca agcagagctt ccctgaggga
     7561 tttacctggg agagagtgac aacttatgaa gatggcggag tgctgaccgc cacacaagat
     7621 accagcctgc aggacggctg tctgatctac aacgtgaaga tcagaggcgt gaacttcacc
     7681 agcaacggcc ctgtgatgca gaaaaaaacc ctgggctggg aggcctttac agagacactg
     7741 taccccgctg atggaggcct ggaaggtaga aacgacatgg ccctgaaact ggtcggcggc
     7801 agccacctga ttgccaatat caagaccaca taccggagca agaaaccagc taaaaacctg
     7861 aagatgcctg gcgtttacta cgtggattac agactggagc ggatcaagga agccaacaac
     7921 gagacatatg tggaacagca cgaggtggcc gtagctagat actgcgacct gccttctaag
     7981 cttggccaca agctgaactg ataagcggcc gcactcctca ggtgcaggct gcctatcaga
     8041 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     8101 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     8161 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     8221 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     8281 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     8341 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     8401 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     8461 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     8521 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     8581 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8641 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8701 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     8761 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8821 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8881 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     8941 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9061 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9121 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9181 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9241 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9481 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9721 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10021 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10081 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10141 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10201 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10261 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10321 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10381 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10441 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10501 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10561 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10621 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10681 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10741 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10801 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10861 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10921 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10981 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11041 cgcacatttc cccgaaaagt gccacctggg t
//
