LOCUS       sequence-437209-       11048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRcharm Kv2 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Neumann EN, Bertozzi TM, Wu E, Serack F, Harvey JW, Brauer PP, 
            Pirtle CP, Coffey A, Howard M, Kamath N, Lenz K, Guzman K, Raymond 
            MH, Khalil AS, Deverman BE, Minikel EV, Vallabh SM, Weissman JS
  TITLE     Brainwide silencing of prion protein by AAV-mediated delivery of an 
            engineered compact epigenetic editor.
  JOURNAL   Science. 2024 Jun 28;384(6703):ado7082. doi: 
            10.1126/science.ado7082. Epub 2024 Jun 28.
  PUBMED    38935715
REFERENCE   2  (bases 1 to 11048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-28"; volume: "384(6703):ado7082. doi"; pages: " 
            10.1126/science.ado7082. Epub 2024 Jun 28"
COMMENT     Sequence Label: pENN236_CRISPRcharm_Kv2
FEATURES             Location/Qualifiers
     source          1..11048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1769..1778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1964..2149
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             3077..3097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3098..3124,3125..3127,3128..5614,5615..5617,
                     5618..7201)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3098..7201
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7202..7222
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7223..7279
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(8067..8086)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8132..8187
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8186..8205)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8548..8564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8548..8564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8561..8583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8572..8588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8596..8602,8603..8620,8621..8626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8641..8662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8721..8916
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8767..8902
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8922..9056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8959..8978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9013..9032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9124..9141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9295..9883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9375..9394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10054..10845,10846..10914))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10677..10696
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10915..11019)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      421 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      481 atgggggcgg gggggggggg gggcgcgcgc cgggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc tgcagtcgac
     1741 ggtaccgcgg gcccgggatc caccggtcgc caccatggcc aggaccaaac aaactgccag
     1801 aaagagcacc ggggggaaag cgccccgaaa acagctggcg accaaagcag cccgcaaaag
     1861 cgcaccggga ggtgcaagca gtggggcagg aagttcctca ggaggaagtg cggcgggatc
     1921 cggaagcggt ggcgcatcaa gtggcgccgc aagtagcagt gccagaaccc tggtgacctt
     1981 caaggacgtg ttcgtggact tcaccagaga agaatggaag ctgctggata ccgctcagca
     2041 gatcgtgtac agaaacgtga tgctggaaaa ctacaagaat ctggtgtccc tgggctacca
     2101 gctgaccaag cctgacgtga tcctgagact ggaaaaggga gaggagcctt ccggctctga
     2161 aacccctgga acctccgaga gcgccacacc tgaaagctcc cctgttgaga tctacaagac
     2221 cgtgtccgcc tggaagcggc agccaatcag agtgctgagc ctgttcggca atattgacaa
     2281 ggaactgaag tctctgggct tcctggaaag cggcagccac agcgagggcg gcacactgaa
     2341 gtacgtggaa gatgtgacca acgtggtgcg gagagatgtg gaaaagtggg gacctttcga
     2401 cctgctgtac ggcagcaccc agcctctggg cggaagctgc gacaggtgtc ctggctggta
     2461 catgttccaa tttcatagaa tcctgcagta cgccagacct agacaggagt ctcagccctt
     2521 cttctggatc ttcatggata acctgctgct cacagaggac gaccagatga ccaccgtgcg
     2581 gttcctgcaa acagaggccg tcaccctgcg cgacgtgaga tctagagtgc tgcagaacgc
     2641 cgtccgggtg tggagcaaca tccccggtct taaaagcaag cacgaggctc tgtccccaaa
     2701 agaggaagag agcctgcagg gccacgtgcg gaccagatct aagctggccg ctcctaaagt
     2761 ggaccccctg gtgaagaact gcctgctgcc tctgagagag tactttaagt atttcagcca
     2821 gagcagcctg cccctgggcg gcccttctag cggtgctcca cctccaagcg gcggctcgcc
     2881 agccggcagc cctaccagca ccgaggaggg cacaagcgag agcgcaacac ctgagagcgg
     2941 ccccggcaca agcaccgagc cttctgaagg aagcgcccct gggagccctg ccggcagccc
     3001 caccagcaca gaagagggca cctctaccga gcccagcgag ggtagcgccc ctggcacaag
     3061 cacagaaccc tctgagccta agaaaaagcg gaaggtgatg gacaagaagt acagcatcgg
     3121 cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     3181 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     3241 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     3301 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     3361 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     3421 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     3481 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     3541 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     3601 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     3661 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     3721 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     3781 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     3841 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     3901 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     3961 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     4021 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     4081 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     4141 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     4201 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     4261 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     4321 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     4381 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     4441 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     4501 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     4561 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     4621 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     4681 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     4741 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     4801 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     4861 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     4921 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     4981 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     5041 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     5101 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     5161 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     5221 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     5281 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     5341 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     5401 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     5461 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     5521 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     5581 ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga gctttctgaa
     5641 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     5701 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     5761 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     5821 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     5881 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     5941 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     6001 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     6061 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     6121 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     6181 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     6241 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     6301 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     6361 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     6421 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     6481 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     6541 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     6601 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     6661 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     6721 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     6781 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     6841 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     6901 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     6961 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     7021 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     7081 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     7141 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggtga
     7201 ccccaagaag aagcggaagg tggccacaaa tttcagcctg ctgaagcagg ccggcgacgt
     7261 ggaagagaat cctggcccca gcgagctgat caaagagaac atgcacatga agctgtacat
     7321 ggaaggcacc gtggacaacc atcacttcaa gtgcaccagc gagggcgagg gcaagcccta
     7381 cgagggcaca cagaccatga gaatcaaggt ggtggaagga ggcccactgc ccttcgcctt
     7441 cgacatcctg gccaccagct ttctgtacgg ctcaaagacc ttcatcaacc acacccaggg
     7501 catccctgat ttcttcaagc agagcttccc tgagggattt acctgggaga gagtgacaac
     7561 ttatgaagat ggcggagtgc tgaccgccac acaagatacc agcctgcagg acggctgtct
     7621 gatctacaac gtgaagatca gaggcgtgaa cttcaccagc aacggccctg tgatgcagaa
     7681 aaaaaccctg ggctgggagg cctttacaga gacactgtac cccgctgatg gaggcctgga
     7741 aggtagaaac gacatggccc tgaaactggt cggcggcagc cacctgattg ccaatatcaa
     7801 gaccacatac cggagcaaga aaccagctaa aaacctgaag atgcctggcg tttactacgt
     7861 ggattacaga ctggagcgga tcaaggaagc caacaacgag acatatgtgg aacagcacga
     7921 ggtggccgta gctagatact gcgacctgcc ttctaagctt ggccacaagc tgaactgata
     7981 agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     8041 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     8101 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     8161 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     8221 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     8281 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     8341 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     8401 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     8461 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     8521 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8581 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8641 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8701 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8761 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8821 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8881 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     8941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     9061 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9721 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9961 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10021 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10081 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10141 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10201 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10261 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10321 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10381 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10441 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10501 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10561 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10621 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10681 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10741 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10801 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10861 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10921 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10981 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11041 acctgggt
//
