LOCUS       sequence-437215-        3251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heterologous expression of SSAT1 under endogenous speG E. coli 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Mayers JR, Varon J, Zhou RR, Daniel-Ivad M, Beaulieu C, Bholse A, 
            Glasser NR, Lichtenauer FM, Ng J, Vera MP, Huttenhower C, Perrella 
            MA, Clish CB, Zhao SD, Baron RM, Balskus EP
  TITLE     A metabolomics pipeline highlights microbial metabolism in 
            bloodstream infections.
  JOURNAL   Cell. 2024 Jun 11:S0092-8674(24)00579-8. doi: 
            10.1016/j.cell.2024.05.035.
  PUBMED    38885650
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Jun 11:S0092-8674(24)00579-8. doi: 10.1016/j.cell.2024.05.035."
COMMENT     Sequence Label: pTrcHis_VectorA_short_speGpromoter_SAT1 (human)
FEATURES             Location/Qualifiers
     source          1..3251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      299..887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     788..807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1041..1058
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2203..2289
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2381..2408
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2427..2518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2519..2587,2588..3251,1..128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2737..2756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
       61 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
      121 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
      181 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
      241 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
      301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
      361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
      421 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
      481 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
      541 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
      601 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      661 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      721 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      781 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      841 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      901 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      961 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     1021 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     1081 ggtattttct ccaaaatagt aacgttgttt taatcatctt tgtctcttat tgacatcatg
     1141 tatagttata gggcgacata atatcatcaa tataaacacc ctcctggtac gttttgcgtc
     1201 cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat tatgaaaatc
     1261 actctcagca aacgaatcgg cctgctcgct attctgctgc cttgcgcact ggcattgagc
     1321 acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag tgcacaaagc
     1381 cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct gcgccagaaa
     1441 gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga taaccgcgat
     1501 aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt gcgctgcctg
     1561 gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag gaatgtaagg
     1621 acacgttatg gccaaatttg ttattcgtcc ggcaaccgca gcagattgta gcgatattct
     1681 gcgtctgatt aaagaactgg ccaaatacga gtatatggaa gaacaggtta tcctgaccga
     1741 aaaagatctg ctggaagatg gttttggtga acatccgttt tatcattgtc tggttgcaga
     1801 agttccgaaa gaacattgga caccggaagg tcatagcatt gttggttttg ccatgtacta
     1861 tttcacctat gatccgtgga ttggcaaact gctgtatctg gaagatttct ttgtgatgag
     1921 cgattatcgc ggttttggta ttggttccga aattctgaaa aatctgagcc aggttgcaat
     1981 gcgttgtcgt tgtagcagca tgcattttct ggtggccgaa tggaatgaac cgagcattaa
     2041 tttctataaa cgtcgtggtg caagcgatct gagcagcgaa gaaggttggc gtctgtttaa
     2101 aatcgataaa gaatacctgc tgaaaatggc caccgaagaa tagccgtagc gccgatggta
     2161 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2281 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     2341 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     2401 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
     2461 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2521 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2581 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2641 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2701 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2761 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2821 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2881 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2941 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3001 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3061 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3121 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3181 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3241 cggtatcatt g
//
