LOCUS       sequence-437222-       11855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and 
            MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site 
            located upstream of pEFS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Neumann EN, Bertozzi TM, Wu E, Serack F, Harvey JW, Brauer PP, 
            Pirtle CP, Coffey A, Howard M, Kamath N, Lenz K, Guzman K, Raymond 
            MH, Khalil AS, Deverman BE, Minikel EV, Vallabh SM, Weissman JS
  TITLE     Brainwide silencing of prion protein by AAV-mediated delivery of an 
            engineered compact epigenetic editor.
  JOURNAL   Science. 2024 Jun 28;384(6703):ado7082. doi: 
            10.1126/science.ado7082. Epub 2024 Jun 28.
  PUBMED    38935715
REFERENCE   2  (bases 1 to 11855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-28"; volume: "384(6703):ado7082. doi"; pages: " 
            10.1126/science.ado7082. Epub 2024 Jun 28"
COMMENT     Sequence Label: pTMB352_ZFcharm_Kv1_split-inteins
FEATURES             Location/Qualifiers
     source          1..11855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(114..133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      927..1515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1416..1435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1669..1686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1803..1824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1839..1844,1845..1862,1863..1869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1877..1893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1882..1904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1901..1917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1901..1917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1936..1956
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1938..1956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1984..2210
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2211..2391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2438..2563
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3056..3289
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3474..3518
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3749..3959
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3771..3967
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3818..3953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3999..4601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3999..4018)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4495..4515
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4643..4760
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5352..5563
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5574..5583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5769..5954
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             6915..6935
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7251..7304
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7974..7994
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7995..8051
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8790..8810
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8811..8867
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8868..9578
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9013..9031)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9281..9300
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(9602..9635)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9691..10279
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9744..9764)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10162..10173)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10351..10584
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10662..10783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10699..10718)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10753..10772
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10823..10958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10978..10997)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10979..10997)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11007..11024)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11007..11023)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11016..11038)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11165..11620
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11252..11271)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11462..11483
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11646..11750
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11751..11819,11820..11855,1..756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
       61 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     1801 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     1861 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     1921 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     1981 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     2041 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     2101 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     2161 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     2221 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     2281 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     2341 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     2401 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     2461 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     2521 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     2581 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     2641 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     2701 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     2761 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     2821 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     2881 taagaccacc gcacagcaag cggcctctga tcttcagacc tggaggagga gatatgaggg
     2941 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     3001 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     3061 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     3121 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     3181 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     3241 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     3301 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     3361 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     3421 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     3481 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     3541 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3601 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3661 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac
     3721 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa
     3781 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3841 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3901 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3961 ttgcaaaaag ctttgaagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg
     4021 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4081 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4141 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     4201 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     4261 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     4321 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     4381 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     4441 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     4501 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     4561 cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta actgcgccaa ttctgcagac
     4621 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     4681 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     4741 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     4801 tcccctcctc cttgacaatg gccatgccag ccaggggtgg ggcaccaggg gttcctgcag
     4861 gcgcagtccc tggctggccg gtagctgcag cggcagcgcc acgttcaata acaataaggt
     4921 tgtagtcctc agtggtactg cctgggaaga ccttgaagac gggtggctgg ctgtcagctg
     4981 tgaggtccag gtccaggcgt tcaaggctca ctcgtggcac cttgcgcata aggccgctca
     5041 cctcgccctc acctgagcgg gaccgtttca cacctgacac atggggctct gcacttgagt
     5101 agggatcatc tcctgaccca aagccatagc cttcctgcac ctccatgggc tggctgctgc
     5161 cagagccctg cttgctcagg ggccctgggg ctcttggagg ggccatgggt gcagggcctg
     5221 ttgagttagt gccaggacgc tctgccacaa tcttgccaaa ggcctcggca ctcttggtcc
     5281 aggcattgag gtcccactga aacttcatct cgccatgtgg ctccacggga tccgacgtcg
     5341 aagtagccgt agggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     5401 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     5461 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     5521 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggctaccg gtcgccacca
     5581 tggccaggac caaacaaact gccagaaaga gcaccggggg gaaagcgccc cgaaaacagc
     5641 tggcgaccaa agcagcccgc aaaagcgcac cgggaggtgc aagcagtggg gcaggaagtt
     5701 cctcaggagg aagtgcggcg ggatccggaa gcggtggcgc atcaagtggc gccgcaagta
     5761 gcagtgccag aaccctggtg accttcaagg acgtgttcgt ggacttcacc agagaagaat
     5821 ggaagctgct ggataccgct cagcagatcg tgtacagaaa cgtgatgctg gaaaactaca
     5881 agaatctggt gtccctgggc taccagctga ccaagcctga cgtgatcctg agactggaaa
     5941 agggagagga gccttccggc tctgaaaccc ctggaacctc cgagagcgcc acacctgaaa
     6001 gctcccctgt tgagatctac aagaccgtgt ccgcctggaa gcggcagcca atcagagtgc
     6061 tgagcctgtt cggcaatatt gacaaggaac tgaagtctct gggcttcctg gaaagcggca
     6121 gccacagcga gggcggcaca ctgaagtacg tggaagatgt gaccaacgtg gtgcggagag
     6181 atgtggaaaa gtggggacct ttcgacctgc tgtacggcag cacccagcct ctgggcggaa
     6241 gctgcgacag gtgtcctggc tggtacatgt tccaatttca tagaatcctg cagtacgcca
     6301 gacctagaca ggagtctcag cccttcttct ggatcttcat ggataacctg ctgctcacag
     6361 aggacgacca gatgaccacc gtgcggttcc tgcaaacaga ggccgtcacc ctgcgcgacg
     6421 tgagatctag agtgctgcag aacgccgtcc gggtgtggag caacatcccc ggtcttaaaa
     6481 gcaagcacga ggctctgtcc ccaaaagagg aagagagcct gcagggccac gtgcggacca
     6541 gatctaagct ggccgctcct aaagtggacc ccctggtgaa gaactgcctg ctgcctctga
     6601 gagagtactt taagtatttc agccagagca gcctgcccct gggcggccct tctagcggtg
     6661 ctccacctcc aagcggcggc tcgccagccg gcagccctac cagcaccgag gagggcacaa
     6721 gcgagagcgc aacacctgag agcggccccg gcacaagcac cgagccttct gaaggaagcg
     6781 cccctgggag ccctgccggc agccccacca gcacagaaga gggcacctct accgagccca
     6841 gcgagggtag cgcccctggc acaagcacag aaccctctga gaagcgaacc gccgacggat
     6901 ctgaatttga gagcccgaag aaaaagagaa aagtttgtct gagctacgag accgagatcc
     6961 tgaccgttga gtacggccta ctccccatcg gcaagatagt tgagaaaaga atcgagtgca
     7021 ccgtgtacag cgtggacaac aatggcaaca tctatacaca gcctgtggcc cagtggcacg
     7081 acagaggaga gcaggaggtg ttcgagtact gcctggaaga tggatcactg atcagagcca
     7141 cgaaggatca caagttcatg accgtggacg gccagatgct gccaatcgac gagatcttcg
     7201 agcgggagct ggacctaatg agagtcgata acctgcctaa tggctctggc gaaggccggg
     7261 gaagcctgct cacttgcggc gacgtggaag agaatccagg ccctatcaag attgccacca
     7321 gaaagtacct gggcaagcag aacgtgtacg atatcggcgt cggcgagccc cacaacttcg
     7381 ccctgaagaa cggttttatc gccagcaatt gcagtaggcc gggcgagcgc ccgttccaat
     7441 gcaggatatg tatgcggaac ttttctcagc gatccagcct tgtccggcac acacgaacac
     7501 atacgggaga gaaacccttc cagtgtcgga tttgtatgcg aaacttctca gataagagtg
     7561 tgctggcacg acatctgcgc actcatactg ggtcccagaa accgttccaa tgtagaatat
     7621 gcatgaggaa ttttagccaa aggtcaagcc ttgttaggca tacacgcaca cacacaggag
     7681 agaaaccatt tcaatgccgc atatgtatga ggaatttttc ccaaagaaat aatctcgggc
     7741 ggcatcttag gacgcacacg ggctctcaga agccattcca atgcagaatt tgcatgcgga
     7801 acttttccac gcatgcggta cttaccagac atacgagaac gcacacgggc gagaagccat
     7861 tccaatgccg catatgtatg cgaaattttt cagatcgggg taacttgacc cgacacctta
     7921 gaacccatct caggggatca aagcggaccg cagacggaag cgaattcgaa agcccgaaaa
     7981 agaagaggaa agttgccaca aacttctctc tgctgaagca ggccggcgat gtcgaagaaa
     8041 atcccggccc catcaaaatc gccacccgga agtacctggg caaacaaaac gtgtatgaca
     8101 tcggcgtggg cgagcctcat aattttgctt tgaaaaacgg cttcatcgct agcaactgcg
     8161 gagtgcctgc tgctatggcc gagagaccat ttcagtgcag aatctgcatg cgcaatttca
     8221 gcaccagcgg aaatctcaca agacacatcc ggacccatac cggcgagaag ccctttgcct
     8281 gcgatatctg cggcagaaag ttcgctctgt ctcaggacct gaaccggcac accaagatcc
     8341 acacacaccc cagagcccct atccccaagc ctttccagtg ccggatttgc atgcggaact
     8401 tcagcgacag gtccgccctg agcagacata tcagaacaca caccggcgaa aagcctttcg
     8461 cctgtgatat ctgtggcaga aagttcgccc ggaacgacga cagaaaaaag cacacaaaga
     8521 tccacaccgg atctcagaaa cctttccaat gtagaatctg catgagaaac ttcagccaga
     8581 gcggcaacct ggccagacac atccggaccc acacaggcga aaagcctttc gcctgtgata
     8641 tctgcggccg gaaattcgcc cagagaatcg acctgacccg gcacaccaag atccacctga
     8701 gacagaagga cgccgccaga ggcagcggcg gcgacgccaa gagcctgacc gcctggtcca
     8761 agaggaccgc cgacggcagc gagttcgagc ccaagaaaaa gcggaaggtg gctacaaact
     8821 tcagcctgct gaaacaggcc ggcgacgtcg aggaaaatcc tggccccatg gtgagcaagg
     8881 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     8941 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     9001 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     9061 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     9121 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     9181 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     9241 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     9301 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     9361 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     9421 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     9481 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     9541 gccactccac cggcggcatg gacgagctgt acaagtaagc ggccgcgcgt cgagggacct
     9601 aataacttcg tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac
     9661 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     9721 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     9781 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9841 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9901 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9961 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    10021 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    10081 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    10141 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    10201 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    10261 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
    10321 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    10381 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10441 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10501 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10561 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
    10621 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
    10681 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10741 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
    10801 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    10861 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    10921 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
    10981 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
    11041 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    11101 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    11161 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    11221 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    11281 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    11341 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    11401 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    11461 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    11521 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    11581 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    11641 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    11701 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    11761 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    11821 acccagaaac gctggtgaaa gtaaaagatg ctgaa
//
