LOCUS       sequence-43724-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB8a-GFP
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(51..335)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(109..128)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tatgtatatc tccttcttaa aagatctttt gaattcccaa aaaaacgggt atggagaaac
       61 agtagagagt tgcgataaaa agcgtcaggt agaatccgct aatcttatgg ataaaaatgc
      121 tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt
      181 atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt acagaatgct
      241 tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga cgcagtgacg
      301 gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga gtatgaaaag
      361 tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg cccatctggt
      421 ggcgggttta acgccgattg aggccaacgg ttatctcgat ttttttatcg accgaccgct
      481 gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg tgaaaaatca
      541 gggacgagaa tttgtttgcc gaccgggtga tattttgctg ttcccgccag gagagattca
      601 tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt actttcgtcc
      661 gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata cggggttctt
      721 tcgcccggat gaagcgcacc agccgcattt cagcgacctg ttttgggcaa atcattaacg
//
