LOCUS       sequence-437246-        5541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase with mouse liver DNase hypersensitive site # 
            50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Chang TY, Waxman DJ
  TITLE     HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver 
            in vivo.
  JOURNAL   BMC Genomics. 2024 Dec 24;25(1):1240. doi: 
            10.1186/s12864-024-11162-9.
  PUBMED    39716078
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2024-12-24"; volume: "25(1):1240. doi"; pages: " 
            10.1186/s12864-024-11162-9"
COMMENT     Sequence Label: pTYC3_pPromALB_enh50363_FireflyLuc
FEATURES             Location/Qualifiers
     source          1..5541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1393..1402
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1399..3051
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3095..3216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3106..3125)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3160..3179
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3464..3481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3635..4223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3715..3734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4423..5214,5215..5283))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5388..5436
                     /note="synthetic polyadenylation signal"
     misc_feature    5450..5541
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5490..5509
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccc tcatctcaaa tgactacagc atgtgtcatg ttgacacaaa
       61 agctagccag tccagaaacc caggagctcc atctgctctg ccaggtttcg cctctgtcca
      121 cctgacttct gagcctcact actctcttgc ctctcctttt ttctcctccc ttttctccca
      181 tctatggcta tcatgggcca gagagtgaaa tgggggctct ggagaaatta tatctgcaga
      241 ccttaaaaaa acctggatca gggaatgaac aggcagagac aaacaaaaga tcatgggaca
      301 ctactcttgt caagccaaat gtaatcacgt ttacccagga cataaagaac atacagagag
      361 gcctccataa tttaagatta gacatgtaaa tacaccccag acatgtaaga atagattagg
      421 tagcacatct agactcagac acagaaatac acatccgtac attcatctca gaaatgacct
      481 ttcaggtatt aaaagcgtac agcacacccg cagtctcttg tcccagtaag tgaacagaca
      541 aaactgagct gacaagggca cacccacctc cataaaacaa gaagcatcac tcccagtgcc
      601 cttttgtcct atgtatctgt ttcatggtgt ccttgccaac atctacagtg agggtaaggg
      661 aatgaggagt gagcagccaa agcagggagt tgctatggct cctcacgctc tggacaatga
      721 gagacaggag gcgggaacag ctgaagttgc ataactgagt ggagaggcgg ggccagcata
      781 aaagatcctg ctggagcgtg gagtgaagtc aacattggtt agaccaggac catggagccc
      841 agtgtcctgc tcctccttgc tctccttgtg ggcttcttgt tactcttagc caggggacac
      901 ccaaagtccc gtggcaactt cccaccagga ccccgtcccc tgcccctctt ggggaacctc
      961 ttgcagatgg acagaggagg cctcctcaag tcttttattc aggtgagctc gaggatatca
     1021 agatctggcc tcggcggcca agctttgctc aaatgggaga caaagagatt aagctcttat
     1081 gtaaaatttg ctgttttaca taactttaat gaatggacaa agtcttgtgc atgggggtgg
     1141 gggtggggtt agaggggaac agctccagat ggcaaacata cgcaagggat ttagtcaaac
     1201 aactttttgg caaagatggt atgattttgt aatggggtag gaaccaatga aatgcgaggt
     1261 aagtatggtt aataatctac agttattggt taaagaagta tattagagcg agtctttctg
     1321 cacacagatc accttcctat caaccccact agcctctggc aaaaagcttg gcaatccggt
     1381 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
     1441 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
     1501 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
     1561 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
     1621 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
     1681 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
     1741 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
     1801 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
     1861 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
     1921 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
     1981 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
     2041 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
     2101 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
     2161 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
     2221 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
     2281 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
     2341 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
     2401 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
     2461 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
     2521 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
     2581 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
     2641 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
     2701 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
     2761 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
     2821 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
     2881 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
     2941 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
     3001 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta ataattctag
     3061 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     3121 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3181 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3241 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3301 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
     3361 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     3421 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     3481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4381 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
     4441 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
     4501 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
     4561 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
     4621 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
     4681 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
     4741 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
     4801 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
     4861 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
     4921 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
     4981 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
     5041 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
     5101 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
     5161 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
     5221 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
     5281 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
     5341 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
     5401 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     5461 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     5521 caggtgccag aacatttctc t
//
