LOCUS       sequence-437247-        9516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length human RELA with an N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Brown Lab
  TITLE     Transcriptional Synergy in Human Aortic Endothelial Cells is 
            Vulnerable to Combination p300/CBP and BET bromodomain Inhibition
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX305_3xFLAG_RELA1
FEATURES             Location/Qualifiers
     source          1..9516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    221..342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(258..277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     312..331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      382..517
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(537..556)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(538..556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(566..583)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(566..582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(575..597)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      724..1179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(811..830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1021..1042
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1205..1309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1310..1378,1379..2170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1528..1547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2341..2929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2830..2849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3083..3100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3217..3238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3253..3258,3259..3276,3277..3283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3291..3307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3296..3318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3315..3331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3315..3331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3350..3370
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3352..3370
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3398..3624
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3625..3805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3852..3977
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4470..4703
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4888..4932
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5163..5373
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5185..5381
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5232..5367
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5413..5811
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5859..5976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6034..6534
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6453..6471
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    6555..6579
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6582..6591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6591..6656
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7953..8324
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     protein_bind    complement(8329..8353)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8361..8681
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             8688..8714
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             8688..8714
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8718..8744
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8766..9354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8819..8839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9237..9248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(9426..9516,1..143)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
       61 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      121 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
      181 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
      241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
      361 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      421 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      481 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
      541 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      601 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      661 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      721 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      781 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      841 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      901 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      961 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1021 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1081 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1141 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     1201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1321 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1381 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1441 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1501 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1561 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1621 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1681 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1741 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1801 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1861 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1921 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2041 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2101 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2161 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2521 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3001 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3061 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3121 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3181 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3241 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3301 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     3361 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     3421 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     3481 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     3541 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     3601 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     3661 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3721 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3781 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     3841 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     3901 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     3961 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4021 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4081 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4141 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4201 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4261 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4321 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4381 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4441 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     4501 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     4561 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     4621 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     4681 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     4741 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     4801 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     4861 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     4921 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     4981 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5041 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5101 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5161 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     5221 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5281 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5341 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     5401 ccggagctta ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     5461 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     5521 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     5581 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     5641 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     5701 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     5761 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agttaacgaa
     5821 ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     5881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     5941 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     6001 gatccacttt ggcgccggct cgaggcctgc aggttgcgcc ttttccaagg cagccctggg
     6061 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     6121 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     6181 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     6241 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     6301 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     6361 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     6421 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     6481 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccaggggcta
     6541 gcatcgattg atcaacaagt ttgtacaaaa aagcaggctt cgccaccatg gactacaaag
     6601 accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat gacaagggtg
     6661 gtggtggttc tatggacgaa ctcttcccac ttatctttcc agcagaaccc gcgcaggcca
     6721 gtggtcctta cgtcgaaata atagagcagc cgaaacagag ggggatgagg ttccggtaca
     6781 aatgcgaggg tcgctcagct gggagtattc cgggggaaag gtctactgac accacgaaga
     6841 ctcatccgac gataaagata aatggttata ctggtcctgg tacagttcgc atttcactcg
     6901 tcaccaaaga ccctccccat cgcccacatc cacacgagct cgttggcaag gattgtcggg
     6961 atggttttta cgaagccgag ctgtgcccgg accgctgcat tcattcattt caaaatcttg
     7021 gaattcaatg tgtaaagaag cgagacctgg agcaggcaat ctcacagagg atacagacaa
     7081 ataataatcc gtttcaagta cccattgaag agcagcgagg cgattacgac cttaacgccg
     7141 ttcggttgtg tttccaagtg accgttcggg acccctctgg aagaccgttg agactgcccc
     7201 cagtcctctc tcatcccatc tttgacaaca gggctcctaa cactgccgag cttaagatat
     7261 gtcgcgtcaa ccggaacagt gggagttgct tgggtggtga cgagatcttc ctgctttgcg
     7321 ataaggtgca gaaggaggat atcgaagtgt attttactgg ccccggctgg gaggcccggg
     7381 gctcttttag tcaagctgat gtacataggc aagtagcgat cgtgttccgc accccgcctt
     7441 atgcggatcc gtctcttcag gcacccgtcc gggtaagcat gcagctccgc agaccgtcag
     7501 accgcgagct cagcgaaccc atggaatttc agtatctccc agatacagat gatcggcata
     7561 gaatagaaga gaaaagaaaa aggacgtatg aaactttcaa gagtataatg aaaaaatcac
     7621 cattttcagg gcccacggac cctcgcccgc caccgcggcg aatagcagtg ccctctagaa
     7681 gtagtgcgag cgtaccaaag cctgcaccgc aaccgtaccc gttcacatcc tccctgagca
     7741 ctattaacta tgacgaattc cctacaatgg tattcccttc agggcaaata tcacaagctt
     7801 ctgcgttggc gccggcgccg ccacaagtcc tcccgcaagc tcctgcacct gcgccggccc
     7861 cagccatggt cagcgcattg gcacaggctc cagcaccagt accagtattg gcacccggcc
     7921 cccctcaggc cgtggcccct ccggctccca agcccactca agcaggagaa ggaactctgt
     7981 ccgaggcact ccttcaactg cagtttgatg atgaagattt gggcgccttg ctgggcaact
     8041 ccactgaccc ggccgttttt accgacctcg cgtccgtaga taatagtgaa ttccagcagc
     8101 ttctcaacca aggaattcct gtagcaccac atacgaccga accgatgctt atggagtacc
     8161 cggaggccat tactcggctt gtaacgggcg ctcagcgacc gcctgaccca gcacctgcac
     8221 ctctgggggc acctggtctg cctaacggac ttctgagtgg tgatgaagac tttagtagca
     8281 ttgctgacat ggacttcagc gctctcttga gccagatctc ttcctaggac ccagctttct
     8341 tgtacaaagt ggttaccggt ggagtgcagg tggaaaccat ctccccagga gacgggcgca
     8401 ccttccccaa gcgcggccag acctgcgtgg tgcactacac cgggatgctt gaagatggaa
     8461 agaaagttga ttcctcccgg gacagaaaca agccctttaa gtttatgcta ggcaagcagg
     8521 aggtgatccg aggctgggaa gaaggggttg cccagatgag tgtgggtcag agagccaaac
     8581 tgactatatc tccagattat gcctatggtg ccactgggca cccaggcatc atcccaccac
     8641 atgccactct cgtcttcgat gtggagcttc taaaactgga aggcggctac ccctacgacg
     8701 tgcccgacta cgccggctat ccgtatgatg tcccggacta tgcaggctaa acgcgttaag
     8761 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     8821 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8881 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8941 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     9001 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     9061 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     9121 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     9181 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     9241 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     9301 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     9361 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     9421 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     9481 gtctctctgg ttagaccaga tctgagcctg ggagct
//
