LOCUS       sequence-437257-       14248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral-mediated stable expression of SARS-CoV-2 NSP3 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 14248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLEX_307-SARS-CoV-2-NSP3-V5
FEATURES             Location/Qualifiers
     source          1..14248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     385..405
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    457..481
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6320..6344)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6352..6393
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6444..7032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6497..6517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6915..6926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7104..7337
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7415..7536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7452..7471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7506..7525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7576..7711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7731..7750)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7732..7750)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7760..7777)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7760..7776)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7769..7791)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7918..8373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8005..8024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8215..8236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8399..8503
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8504..8572,8573..9364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8722..8741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9535..10123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10024..10043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10277..10294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10411..10432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10447..10452,10453..10470,10471..10477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10485..10501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10490..10512
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10509..10525
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10509..10525
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10544..10564
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10546..10564
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10592..10818
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10819..10999
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11046..11171
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11664..11897
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12082..12126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12357..12567
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12379..12575
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12426..12561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12607..13209
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12607..12626)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13103..13123
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13252..13369
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(13507..14248,1..437)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(13738..14248,1..428)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
       61 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
      121 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
      181 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
      241 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
      301 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
      361 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
      421 catttcaggt gtcgtgaggc tagcatcgat tgatcaacaa gtttgtacaa aaaagttggc
      481 atggccccta ccaaggtgac cttcggagac gacaccgtga tcgaggtgca gggttacaag
      541 agcgtgaaca tcaccttcga gctggacgaa cgcatcgaca aggtgctgaa cgaaaagtgc
      601 tccgcttaca ccgtggagct gggcaccgag gtgaacgaat tcgcctgcgt ggtggccgac
      661 gctgtgatca agaccctgca gccagtgtcc gaactgctga cccctctggg tatcgacctg
      721 gacgagtggt ctatggctac ctactacctg ttcgacgaga gcggcgaatt caagctggcc
      781 tctcacatgt actgcagctt ctaccctccc gacgaagacg aggaagaggg tgactgcgaa
      841 gaggaagagt tcgagccttc cactcagtac gagtacggca ccgaagacga ctaccagggc
      901 aagcctctgg aattcggagc cacctctgcc gctctgcagc ctgaagagga acaggaggaa
      961 gactggctgg acgacgactc ccagcagacc gtgggtcagc aggacggctc tgaagacaac
     1021 cagaccacca ccatccagac catcgtggag gtgcagcctc agctggagat ggaactgacc
     1081 ccagtggtgc agaccatcga ggtgaacagc ttctccggct acctgaagct gaccgacaac
     1141 gtgtacatca agaacgctga catcgtggag gaagccaaga aggtgaaacc aaccgtggtg
     1201 gtgaacgccg ctaacgtgta cctgaagcac ggcggaggtg tggctggagc tctgaacaag
     1261 gctaccaaca acgccatgca ggtggaatcc gacgactaca tcgctaccaa cggtcctctg
     1321 aaggtgggcg gatcctgcgt gctgtctggc cacaacctgg ccaagcactg cctgcacgtg
     1381 gtgggaccaa acgtgaacaa gggagaggac atccagctgc tgaagagcgc ctacgaaaac
     1441 ttcaaccagc acgaggtgct gctggctcca ctgctgtctg ccggaatctt cggtgctgat
     1501 cccatccaca gcctgcgcgt gtgcgtggac accgtgagga ccaacgtgta cctggccgtg
     1561 ttcgacaaga acctgtacga caagctggtg agctccttcc tggagatgaa gtccgaaaag
     1621 caggtggagc agaagatcgc cgagatcccc aaggaggaag tgaagccttt catcaccgaa
     1681 tctaagccca gcgtggagca gagaaagcag gacgacaaga agatcaaggc ctgcgtggag
     1741 gaagtgacca ccaccctgga ggaaaccaag ttcctgaccg agaacctgct gctgtacatc
     1801 gacatcaacg gcaacctgca ccctgactcc gctaccctgg tgtctgacat cgacatcacc
     1861 ttcctgaaga aggacgcccc ctacatcgtg ggcgacgtgg tgcaggaagg agtgctgacc
     1921 gctgtggtca tccctaccaa gaaggccggt ggcaccaccg agatgctggc caaggctctg
     1981 cgtaaggtgc caaccgacaa ctacatcacc acctaccccg gtcagggcct gaacggttac
     2041 accgtggagg aagctaagac cgtgctgaag aagtgcaagt ctgccttcta catcctgccc
     2101 tccatcatct ctaacgagaa gcaggaaatc ctgggcaccg tgagctggaa cctgcgcgaa
     2161 atgctggccc acgctgagga aaccaggaag ctgatgcccg tgtgcgtgga gaccaaggcc
     2221 atcgtgagca ccatccagag aaagtacaag ggcatcaaga tccaggaggg tgtggtggac
     2281 tacggcgctc gtttctactt ctacacctcc aagaccaccg tggcctctct gatcaacacc
     2341 ctgaacgacc tgaacgagac cctggtgacc atgccactgg gatacgtgac ccacggtctg
     2401 aacctggagg aagccgctag atacatgcgt tccctgaagg tgcctgctac cgtgagcgtg
     2461 agcagccctg acgctgtgac cgcctacaac ggatacctga cctcctctag caagacccct
     2521 gaggaacact tcatcgaaac catcagcctg gccggttcct acaaggactg gtcttacagc
     2581 ggacagtcca cccagctggg catcgagttc ctgaagcgcg gagacaagag cgtgtactac
     2641 acctccaacc caaccacctt ccacctggac ggtgaagtga tcaccttcga caacctgaag
     2701 accctgctgt ctctgcgcga ggtgaggacc atcaaggtgt tcaccaccgt ggacaacatc
     2761 aacctgcaca cccaggtggt ggacatgagc atgacctacg gacagcagtt cggtcctacc
     2821 tacctggacg gagctgacgt gaccaagatc aagccacaca actcccacga gggcaagacc
     2881 ttctacgtgc tgcctaacga tgacaccctg agagtggagg ctttcgaata ctaccacacc
     2941 accgacccat ccttcctggg caggtacatg tctgccctga accacaccaa gaagtggaag
     3001 tacccccagg tgaacggact gaccagcatc aagtgggccg acaacaactg ctacctggcc
     3061 accgctctgc tgaccctgca gcagatcgaa ctgaagttca acccacccgc tctgcaggac
     3121 gcttactaca gagcccgtgc tggcgaggcc gctaacttct gcgctctgat cctggcctac
     3181 tgcaacaaga ccgtgggcga actgggagac gtgagagaga ccatgagcta cctgttccag
     3241 cacgccaacc tggactcctg caagcgtgtg ctgaacgtgg tgtgcaagac ctgcggccag
     3301 cagcagacca ccctgaaggg agtggaagct gtgatgtaca tgggtaccct gtcctacgag
     3361 cagttcaaga agggcgtgca gatcccttgc acctgcggaa agcaggccac caagtacctg
     3421 gtgcagcagg aaagcccatt cgtgatgatg tccgctcctc cagcccagta cgaactgaag
     3481 cacggcacct tcacctgcgc ttccgagtac accggcaact accagtgcgg acactacaag
     3541 cacatcacct ctaaggagac cctgtactgc atcgacggag ccctgctgac caagtcctct
     3601 gaatacaagg gtcccatcac cgacgtgttc tacaaggaga acagctacac caccaccatc
     3661 aagcccgtga cctacaagct ggacggcgtg gtgtgcaccg aaatcgaccc taagctggac
     3721 aactactaca agaaggacaa ctcttacttc accgagcagc ctatcgacct ggtgccaaac
     3781 cagccctacc ctaacgccag cttcgacaac ttcaagttcg tgtgcgacaa catcaagttc
     3841 gctgacgacc tgaaccagct gaccggatac aagaagcctg cctctagaga gctgaaggtg
     3901 accttcttcc cagacctgaa cggcgacgtg gtggctatcg actacaagca ctacacccca
     3961 agcttcaaga agggagccaa gctgctgcac aagcccatcg tgtggcacgt gaacaacgct
     4021 accaacaagg ccacctacaa gcctaacacc tggtgcatcc gttgcctgtg gtctaccaag
     4081 ccagtggaaa cctccaactc tttcgacgtg ctgaagagcg aggacgctca gggcatggac
     4141 aacctggcct gcgaagacct gaagcccgtg agcgaggaag tggtggagaa cccaaccatc
     4201 cagaaggacg tgctggaatg caacgtgaag accaccgagg tggtgggaga catcatcctg
     4261 aagccagcta acaacagcct gaagatcacc gaggaagtgg gtcacaccga cctgatggcc
     4321 gcttacgtgg acaacagctc cctgaccatc aagaagccca acgagctgag cagggtgctg
     4381 ggtctgaaga ccctggctac tcacggtctg gctgctgtga actctgtgcc atgggacacc
     4441 atcgctaact acgccaagcc tttcctgaac aaggtggtgt ccaccaccac caacatcgtg
     4501 accagatgtc tgaaccgcgt gtgcaccaac tacatgccct acttcttcac cctgctgctg
     4561 cagctgtgca ccttcaccag aagcaccaac tcccgtatca aggctagcat gcctaccacc
     4621 atcgccaaga acaccgtgaa gagcgtggga aagttctgcc tggaggcttc cttcaactac
     4681 ctgaagtctc caaacttcag caagctgatc aacatcatca tctggttcct gctgctctcc
     4741 gtgtgcctgg gttccctgat ctactctacc gccgctctgg gagtgctgat gtctaacctg
     4801 ggtatgccta gctactgcac tggttacaga gagggatacc tgaacagcac caacgtgacc
     4861 atcgccacct actgcaccgg cagcatccca tgctccgtgt gcctgtctgg actggacagc
     4921 ctggacacct acccctccct ggaaaccatc cagatcacca tctctagctt caagtgggac
     4981 ctgaccgcct tcggtctggt ggctgagtgg ttcctggcct acatcctgtt caccaggttc
     5041 ttctacgtgc tgggcctggc cgctatcatg cagctgttct tctcctactt cgctgtgcac
     5101 ttcatcagca actcctggct gatgtggctg atcatcaacc tggtgcagat ggctcccatc
     5161 tctgccatgg tgcgcatgta catcttcttc gccagcttct actacgtgtg gaagtcctac
     5221 gtgcacgtgg tggacggttg caactcctct acctgcatga tgtgctacaa gcgcaacagg
     5281 gctaccaggg tggagtgcac caccatcgtg aacggcgtgc gcaggtcctt ctacgtgtac
     5341 gccaacggcg gcaagggatt ctgcaagctg cacaactgga actgcgtgaa ctgcgacacc
     5401 ttctgcgctg gctccacctt catctctgac gaggtggcca gagacctgag cctgcagttc
     5461 aagcgtccaa tcaaccccac cgaccagagc tcctacatcg tggactctgt gaccgtgaag
     5521 aacggtagca tccacctgta cttcgacaag gctggccaga agacctacga gcgccactct
     5581 ctgagccact tcgtgaacct ggacaacctg agggccaaca acaccaaggg cagcctgcct
     5641 atcaacgtga tcgtgttcga cggaaagtcc aagtgcgagg aatctagcgc caagtccgcc
     5701 agcgtgtact acagccagct gatgtgccag ccaatcctgc tgctggacca ggctctggtg
     5761 tctgacgtgg gagacagcgc cgaagtggcc gtgaagatgt tcgacgccta cgtgaacacc
     5821 ttctcctcta ccttcaacgt gcctatggag aagctgaaga ccctggtggc tactgctgag
     5881 gctgaactgg ctaagaacgt gtccctggac aacgtgctga gcaccttcat ctccgccgct
     5941 aggcagggtt tcgtggactc tgacgtggaa accaaagacg tggtggagtg cctgaagctg
     6001 tctcaccaga gcgacatcga agtgaccggc gactcctgca acaactacat gctgacctac
     6061 aacaaggtgg agaacatgac cccaagagac ctgggagctt gcatcgactg ctccgcccgt
     6121 cacatcaacg ctcaggtggc caagtctcac aacatcgccc tgatctggaa cgtgaaggac
     6181 ttcatgagcc tgtccgagca gctgcgcaag cagatcagga gcgccgctaa gaagaacaac
     6241 ctgcccttca agctgacctg cgctaccacc agacaggtgg tgaacgtggt gaccaccaag
     6301 atcgccctga agggcggata cccaactttc ttgtacaaag tggttgatat cggtaagcct
     6361 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtac cggttaagtc
     6421 gacaatcaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6481 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6541 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6601 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6661 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6721 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6781 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6841 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6901 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6961 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7021 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     7081 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7141 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7201 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7261 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7321 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     7381 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7441 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7501 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7561 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7621 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7681 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     7741 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7801 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7861 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     7921 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7981 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8041 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8101 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8161 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8221 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8281 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8341 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     8401 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8461 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8521 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8581 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8641 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8701 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8761 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8821 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8881 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8941 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9001 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9061 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9121 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9181 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9241 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9301 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9361 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9421 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9481 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9541 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9601 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9661 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9721 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9781 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9841 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9901 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9961 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10021 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10081 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10141 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    10201 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    10261 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    10321 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    10381 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    10441 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    10501 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
    10561 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
    10621 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
    10681 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
    10741 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
    10801 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    10861 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    10921 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    10981 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
    11041 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
    11101 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
    11161 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
    11221 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
    11281 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
    11341 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
    11401 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
    11461 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
    11521 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
    11581 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    11641 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    11701 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    11761 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    11821 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    11881 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    11941 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    12001 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    12061 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    12121 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    12181 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    12241 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
    12301 atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
    12361 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc
    12421 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    12481 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    12541 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag
    12601 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
    12661 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
    12721 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
    12781 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
    12841 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
    12901 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
    12961 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
    13021 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
    13081 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
    13141 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
    13201 gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
    13261 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
    13321 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
    13381 cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca aagatggata
    13441 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
    13501 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
    13561 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
    13621 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
    13681 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
    13741 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
    13801 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
    13861 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
    13921 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
    13981 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
    14041 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
    14101 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
    14161 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
    14221 gtgcctggcc tcgcgccgcc gtgtatcg
//
