LOCUS       sequence-437258-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A single-plasmid-based, easily cured CRISPR/Cas9 system for genome 
            engineering in Pseudomonas putida KT2440.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Wen Q, Chen J, Li J, Dharmasiddhi IPW, Yang M, Xing J, Liu Y
  TITLE     A single-plasmid-based, easily curable CRISPR/Cas9 system for rapid,
            iterative genome editing in Pseudomonas putida KT2440.
  JOURNAL   Microb Cell Fact. 2024 Dec 30;23(1):349. doi: 
            10.1186/s12934-024-02634-4.
  PUBMED    39734219
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2024-12-30"; volume: "23(1):349. doi"; pages: " 
            10.1186/s12934-024-02634-4"
COMMENT     Sequence Label: pBBR-Cas9-Delta-yqhD-HA500
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             201..995
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(255..274)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     865..884
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        join(1119..1124,1125..1142,1143..1148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             1174..5280
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1174..5277
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      5304..5375
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5391..5418
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        5531..5565
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        5586..5661
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(6754..7416)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      7417..8188
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
       61 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
      121 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagattt gatcaagaga
      181 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
      241 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      301 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      361 ccggtgccct gaatgaacta caggacgagg cagcgcggct atcgtggctg gccacgacgg
      421 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      481 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      541 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      601 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
      661 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
      721 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
      781 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
      841 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
      901 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
      961 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     1021 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     1081 aaggttggac tagagaattc atcctgaact tatctagatt tacactttat gcttccggct
     1141 cgtatgtttt ctctggggaa ccatagaaga tcaatggata agaaatactc aataggctta
     1201 gatatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct
     1261 aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg
     1321 gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt
     1381 agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag
     1441 atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa
     1501 gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat
     1561 gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg
     1621 gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg
     1681 attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta
     1741 caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa
     1801 gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc
     1861 cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc
     1921 cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat
     1981 acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg
     2041 tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat
     2101 actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat
     2161 caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa
     2221 atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa
     2281 gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta
     2341 ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct
     2401 attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt
     2461 tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct
     2521 tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct
     2581 gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa
     2641 tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca
     2701 aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat
     2761 gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt
     2821 gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc
     2881 aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct
     2941 tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat
     3001 gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg
     3061 gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa
     3121 cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt
     3181 attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc
     3241 aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa
     3301 aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt
     3361 agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa
     3421 gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca
     3481 actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa
     3541 gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa
     3601 aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat
     3661 attaatcgtt taagtgatta tgatgtcgat cacattgttc cacaaagttt ccttaaagac
     3721 gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac
     3781 gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc
     3841 aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt
     3901 gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag
     3961 catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt
     4021 attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat
     4081 ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta
     4141 aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc
     4201 tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata
     4261 ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa
     4321 attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact
     4381 ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg
     4441 ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca
     4501 attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa
     4561 aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg
     4621 gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg
     4681 gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa
     4741 gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt
     4801 cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca
     4861 agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca
     4921 gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt
     4981 attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa
     5041 gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt
     5101 attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca
     5161 acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat
     5221 caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactaa
     5281 ctcgagtaag gatctccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     5341 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     5401 cgggtgggcc tttctgcgtt tatacctagg cataatgtgc ctgtcaaatg gacgaagcag
     5461 ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaagg
     5521 atcccgatcc ttgacagcta gctcagtcct aggtataata ctagttcggt aaccaaacca
     5581 ttgccgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     5641 aaagtggcac cgagtcggtg ctttttttga attctctaga gtcgacctgc agaagcggca
     5701 ccgggtgatc gtctttcacg aactgttccg gggcactctg gacagtgcca gcgtctatcc
     5761 ccgggaagtc gtcaagactg ctctggagca caacgccgcg gcaaccgtgc tggttcacaa
     5821 ccacccttcc ggtgaggcac tgggtctcag gcagatcctt ctgctacaaa gcgaagtccg
     5881 cgtggtaacg cttggctgca tcagtcgatg cgctgccaat gaagcttctg ctgcttcatt
     5941 agcaaccatc gaaacagcgt tgtatagatt atccacaacc gaaagtagac gaccggtcta
     6001 tttttttgat acggtaaacc tatgactccc gatgccctat catcagtgcc tagcaacaac
     6061 cctcgacatc gcgcggagct tacgctgctt ggcggcaacg cctttggctc gggaccagga
     6121 atgcagacga atgccacacc aacttggttg cctttagaag caagcttcta accaatcttc
     6181 agctatttgg tgcgcaggct cagaaatgaa caacggcggt gtggtaactc gccgagcgac
     6241 acaagacaag ttgccgtttc gcagccctca cgtgttcccg caattctcga ttcttgatcc
     6301 tactaagacc cagaccctcc ctacccgcca actcgcaaac ggcgtagtag atgcgttcgt
     6361 gcatgtcatg gagcagtacc ttacctaccc aatcgatgcc cgcgtgcaag accgcttcgc
     6421 cgaagggctg ctgcaaacac tgatcgaaat cggtccgctg atgctggagg agtctgcgga
     6481 ctacagcacc cgcgccaacc tcatgtggac ggccactctg gcgcttaatg gcttgatcgg
     6541 tgcgggtgtg ccacaggact ggtctacgca catgattggt catgagctga ccgcacagca
     6601 cggcatcgat cacgcacgga cgctggctat cgtgttaccc gcaaatctcg aagttcgccg
     6661 tgaagccaag cgcgccaagt tgctgcaata cgccgactgc agtctagtat agtctggaac
     6721 agcgcactta cgggttgctg cgcaacccaa gtgctaccgg cgcggcagcg tgacccgtgt
     6781 cggcggctcc aacggctcgc catcgtccag aaaacacggc tcatcgggca tcggcaggcg
     6841 ctgctgcccg cgccgttccc attcctccgt ttcggtcaag gctggcaggt ctggttccat
     6901 gcccggaatg ccgggctggc tgggcggctc ctcgccgggg ccggtcggta gttgctgctc
     6961 gcccggatac agggtcggga tgcggcgcag gtcgccatgc cccaacagcg attcgtcctg
     7021 gtcgtcgtga tcaaccacca cggcggcact gaacaccgac aggcgcaact ggtcgcgggg
     7081 ctggccccac gccacgcggt cattgaccac gtaggccgac acggtgccgg ggccgttgag
     7141 cttcacgacg gagatccagc gctcggccac caagtccttg actgcgtatt ggaccgtccg
     7201 caaagaacgt ccgatgagct tggaaagtgt cttctggctg accaccacgg cgttctggtg
     7261 gcccatctgc gccacgaggt gatgcagcag cattgccgcc gtgggtttcc tcgcaataag
     7321 cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc cagtgaccgg gcttgttctt
     7381 ggcttgaatg ccgatttctc tggactgcgt ggccatgctt atctccatgc ggtaggggtg
     7441 ccgcacggtt gcggcaccat gcgcaatcag ctgcaacttt tcggcagcgc gacaacaatt
     7501 atgcgttgcg taaaagtggc agtcaattac agattttctt taacctacgc aatgagctat
     7561 tgcggggggt gccgcaatga gctgttgcgt accccccttt tttaagttgt tgatttttaa
     7621 gtctttcgca tttcgcccta tatctagttc tttggtgccc aaagaagggc acccctgcgg
     7681 ggttccccca cgccttcggc gcggctcccc ctccggcaaa aagtggcccc tccggggctt
     7741 gttgatcgac tgcgcggcct tcggccttgc ccaaggtggc gctgccccct tggaaccccc
     7801 gcactcgccg ccgtgaggct cggggggcag gcgggcgggc ttcgcccttc gactgccccc
     7861 actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc
     7921 gcgagccttg acccgccttc cacttggtgt ccaaccggca agcgaagcgc gcaggccgca
     7981 ggccggaggc ttttccccag agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc
     8041 gcagttggag ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg
     8101 tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt gtccacgggc
     8161 cgagcgaagc gagccagccg gtggccgctc gcggccatcg tccacatatc cacgggctgg
     8221 caagggagcg cagcgaccgc gcagggcgaa gcccggagag caagcccgta gggcgccgca
     8281 gccgccgtag gcggtcacga ctttgcgaag caaagtctag tgagtatact caagcattga
     8341 gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga gccggttgga caccaaaagg
     8401 gaggggcagg catggcggca tacgcgatca tgcgatgcaa gaagctggcg aaaatgggca
     8461 acgtggcggc cagtctcaag cacgcctacc gcgagcgcga gacgcccaac gctgacgcca
     8521 gcaggacgcc agagaacgag cactgggcgg ccagcagcac cgatgaagcg atgggccgac
     8581 tgcgcgagtt gctgccagag aagcggcgca aggacgctgt gttggcggtc gagtacgtca
     8641 tgacggccag cccggaatgg tggaagtcgg ccagccaaga acagcaggcg gcgttcttcg
     8701 agaaggcgca caagtggctg gcggacaagt acggggcgga tcgcatcgtg acggccagca
     8761 tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt ggtgccgctg acgcaggacg
     8821 gcaggctgtc ggccaaggag ttcatcggca acaaagcgca gatgacccgc gaccagacca
     8881 cgtttgcggc tgctgtggcc gatctagggc tgcaacgggg catcgagggc agcaaggcac
     8941 gtcacacgcg cattcaggcg ttctacgagg ccctggagcg gccaccagtg ggccacgtca
     9001 ccatcagccc gcaagcggtc gagccacgcg cctatgcacc gcagggattg gccgaaaagc
     9061 tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc cgaccggctg acaaaagcgg
     9121 ttcggcaggg gtatgagcct gccctacagg ccgccgcagg agcgcgtgag atgcgcaaga
     9181 aggccgatca agcccaagag acggcccgag accttcggga gcgcctgaag cccgttctgg
     9241 acgccctggg gccgttgaat cgggatatgc aggccaaggc cgccgcgatc atcaaggccg
     9301 tgggcgaaaa gctgctgacg gaacagcggg aagtccagcg ccagaaacag gcccagcgcc
     9361 agcaggaacg cgggcgcgca catttccccg aaaagtgcca cctgggatga atgtcagcta
     9421 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcagg
//
