LOCUS       sequence-437262-        9194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9194)
  TITLE     SARS-CoV-2 mutant
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.3_SARS2_JN.1
FEATURES             Location/Qualifiers
     source          1..9194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(181..972,973..1041))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     804..823
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1042..1146)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1221..1240)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1412..1791
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1792..1995
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1946..1966
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1992..2016
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5942..5961)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5986..6034
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6236..6664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6323..6342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6533..6554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6673..6693)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6678..7007
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6858..6993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6920..6939
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7074..7868
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7128..7147)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7738..7757
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8044..8165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8081..8100)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8135..8154
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8214..8230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8214..8230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8227..8249)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8238..8254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8262..8268,8269..8286,8287..8292))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8307..8328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8445..8462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8616..9194,1..10))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8696..8715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
       61 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      181 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
      241 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      301 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      361 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      421 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      481 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      541 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      601 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      661 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      721 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      781 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      841 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      901 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      961 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1021 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     1081 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     1141 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     1201 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     1261 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     1321 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     1381 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     1441 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     1501 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     1561 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     1621 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     1681 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     1741 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     1801 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     1861 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     1921 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     1981 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     2041 tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactgagct ccttaaggtt
     2101 aactctagag aattcggatc catgtttgtg ttcctggtgc tgctgccact ggtgtccagc
     2161 cagtgtgtga tgcctctgtt caacctgatc accacgaccc aatcctacac caactccttc
     2221 accaggggag tctactaccc tgacaaggtg ttcaggtcct ctgtgctgca cctcacccag
     2281 gacctgttcc tgccattctt cagcaatgtg acctggttcc atgccatctc tggcaccaat
     2341 ggcaccaaga ggtttgacaa ccctgtgctg ccattcaatg atggagtcta ctttgccagc
     2401 acagagaaga gcaacatcat caggggctgg atttttggca ccaccctgga cagcaagacc
     2461 cagtccctgc tgattgtgaa caatgccacc aatgtgttca ttaaggtgtg tgagttccag
     2521 ttctgtaatg acccattcct ggacgtctac cacaagaaca acaagtcctg gatggagtct
     2581 gagagtggag tctactcctc tgccaacaac tgtacctttg aatatgtgag ccaaccattc
     2641 ctgatggact tggagggcaa gcagggcaac ttcaagaacc tgagggagtt tgtgttcaag
     2701 aacattgatg gctacttcaa gatttacagc aaacacacac caatcatcgg tagggacttt
     2761 ccacagggct tctctgcctt ggaaccactg gtggacctgc caattggcat caacatcacc
     2821 aggttccaga ccctgctggc tctgaacagg tcctacctga cacctggaga ctcctcctct
     2881 ggctggacag caggagcagc agactactat gtgggctacc tccaaccaag gaccttcctg
     2941 ctgaaataca atgagaatgg caccatcaca gatgctgtgg actgtgccct ggacccactg
     3001 tctgagacca agtgtaccct gaaatccttc acagtggaga agggcatcta ccagaccagc
     3061 aacttcaggg tccaaccaac agagagcatt gtgaggtttc caaacgtcac caacctgtgt
     3121 ccatttcacg aggtgttcaa tgccaccagg tttgcctctg tctatgcctg gaacaggacg
     3181 aggattagca actgtgtggc tgactactct gtgctctaca actttgcccc cttcttcgcc
     3241 ttcaagtgtt atggagtgag cccaaccaaa ctgaatgacc tgtgtttcac caatgtctat
     3301 gctgactcct ttgtgattaa gggaaatgag gtgagtcaga ttgcccctgg acaaacaggc
     3361 aacattgctg actacaacta caaactgcct gatgacttca caggctgtgt gattgcctgg
     3421 aacagcaaca agctggacag caagcactca ggcaactacg actactggta cagatcgttc
     3481 aggaagagca agctgaaacc atttgagagg gacatcagca cagagattta ccaggctggc
     3541 aacaaaccat gtaagggaaa gggcccgaac tgttactttc cactccagtc ctatggcttc
     3601 agaccaacct atggagtggg ccaccaacca tacagggtgg tggtgctgtc ctttgaactg
     3661 ctccatgccc ctgccacagt gtgtggacca aagaagagca ccaacctggt gaagaacaag
     3721 tgtgtgaact tcaacttcaa tggactgaca ggcacaggag tgctgacaaa gagcaacaag
     3781 aagttcctgc cattccaaca gtttggcagg gacattgttg acaccacaga tgctgtgagg
     3841 gacccacaga ccttggagat tctggacatc acaccatgtt cctttggagg agtgtctgtg
     3901 attacacctg gcaccaacac cagcaaccag gtggctgtgc tctaccaggg tgtgaactgt
     3961 actgaggtgt ctgtggctat ccatgctgac caacttacac caacctggag ggtctacagc
     4021 acaggcagca atgtgttcca gaccagggct ggctgtctga ttggagcaga gtatgtgaac
     4081 aactcctatg agtgtgacat cccaattgga gcaggcgtct gtgcctccta ccagacccag
     4141 accaagagcc gtaggagggc aaggtctgtg gcaagccaga gcatcattgc ctacacaatg
     4201 agtctgggag cagagaactc tgtggcttac agcaacaaca gcattgccat cccaaccaac
     4261 ttcaccatct ctgtgaccac agaaattctg cctgtgagta tgaccaagac ctctgtggac
     4321 tgtacaatgt atatctgtgg agacagcaca gagtgtagca acctgctgct ccaatatggc
     4381 tccttctgta cccaacttaa gagggctctg acaggcattg ctgtggaaca ggacaagaac
     4441 acccaggagg tgtttgccca ggtgaagcag atttacaaga cacctccaat caagtacttt
     4501 ggaggcttca acttcagcca gattctgcct gacccaagca agccaagcaa gaggtccttc
     4561 attgaggacc tgctgttcaa caaggtgacc ctggctgatg ctggcttcat caagcaatat
     4621 ggagactgtc tgggagacat tgctgcaagg gacctgattt gtgcccagaa gttcaatgga
     4681 ctgaccgtgc tgcctccact gctgacagat gagatgattg cccaatacac ctctgccctg
     4741 ctggctggca ccatcacctc tggctggacc tttggagcag gagcagccct ccaaatccca
     4801 tttgctatgc agatggctta caggttcaat ggcattggag tgacccagaa tgtgctctat
     4861 gagaaccaga aactgattgc caaccagttc aactctgcca ttggcaagat tcaggactcc
     4921 ctgttcagca cagcctctgc cctgggcaaa ctccaagatg tggtgaacca taatgcccag
     4981 gctctgaaca ccctggtgaa gcaactttcc agcaagtttg gagccatctc ctctgtgctg
     5041 aatgacatcc tgagcagact ggacaaggtg gaggctgagg tccagattga cagactgatt
     5101 acaggcagac tccaatccct ccaaacctat gtgacccaac aacttatcag ggctgctgag
     5161 attagggcat ctgccaacct ggctgccacc aagatgagtg agtgtgtgct gggacaaagc
     5221 aagagggtgg acttctgtgg caagggctac cacctgatga gttttccaca gtctgcccct
     5281 catggagtgg tgttcctgca tgtgacctat gtgcctgccc aggagaagaa cttcaccaca
     5341 gcccctgcca tctgccatga tggcaaggct cactttccaa gggagggagt gtttgtgagc
     5401 aatggcaccc actggtttgt gacccagagg aacttctatg aaccacagat tatcaccaca
     5461 gacaacacct ttgtgtctgg caactgtgat gtggtgattg gcattgtgaa caacacagtc
     5521 tatgacccac tccaacttga actggactcc ttcaaggagg aactggacaa atacttcaag
     5581 aaccacacca gccctgatgt ggacctggga gacatctctg gcatcaatgc ctctgtggtg
     5641 aacatccaga aggagattga cagactgaat gaggtggcta agaacctgaa tgagtccctg
     5701 attgacctcc aagaactggg caaatatgaa caatacatca agtggccatg gtacatctgg
     5761 ctgggcttca ttgctggact gattgccatt gtgatggtga ccataatgct gtgttgtatg
     5821 acctcctgtt gttcctgtct gaaaggctgt tgttcctgtg gctcctgttg taagtgactc
     5881 gagccatgga agcttgatat ctaactgact gaaccggtgg taccaaacgg gggaggctaa
     5941 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     6001 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     6061 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     6121 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     6181 gcggcaggcc ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg
     6241 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6301 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6361 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6421 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6481 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6541 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6601 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6661 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6841 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6901 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6961 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7021 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     7081 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     7141 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     7201 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     7261 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     7321 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     7381 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     7441 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     7501 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     7561 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     7621 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     7681 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     7741 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     7801 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     7861 tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca
     7921 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7981 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8041 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8101 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8161 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8221 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8281 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8341 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9181 acgcgcagaa aaaa
//
