LOCUS       sequence-437265-        9131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 BA.2.87.1 spike protein for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9131)
  TITLE     SARS-CoV-2 mutant
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.3_SARS2_BA.2.87.1
FEATURES             Location/Qualifiers
     source          1..9131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1936..1956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1982..2006
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5869..5888)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5913..5961
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6163..6591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6250..6269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6460..6481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6600..6620)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6605..6934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6785..6920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6847..6866
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7001..7795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7055..7074)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7665..7684
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7971..8092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8008..8027)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8062..8081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8141..8157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8141..8157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8154..8176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8165..8181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8189..8195,8196..8213,8214..8219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8234..8255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8372..8389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8543..9131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8623..8642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcat ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     2041 atagaagaca ccgggaccga tccagcctcc ggactgagct ccttaaggtt aactctagag
     2101 aattcggatc catgtttgtg ttcctggtgc tgctgccact ggtgtccagc cagtcctaca
     2161 ccaactcctt caccagggga gtctactacc ctgacaaggt gttcaggtcc tctgtgctgc
     2221 acagcaccca ggacctgttc ctgccattct tcagcaatgt gacctggttc catgccatct
     2281 ctggcaccaa tgacaccaag aggtttgaca accctgtgct gccattcaat gatggagtct
     2341 actttgccag cacagagaag ttcaacatca tcaggggctg gatttttggc accaccctgg
     2401 acagcaagac ccagtccctg ctgattgtga acaatgccac caatgcggtg attaaggtgt
     2461 gtgagttcca gttcaagaac aacaagtcct tgatggagtc tgagttcagg gtctactcct
     2521 ctgccaacaa ctgtaccttt gaatatgtga gccaaccatt cctgatggac ttggagggca
     2581 agcagggcaa cttcaagaac ctgagtgagt ttgtgttcaa gaacattgat ggctacttca
     2641 agatttacag caaacacaca ccaatcaacc tgggtagagg cctgccacag ggcttctctg
     2701 ccttggaacc actggtggac ctgccaattg gcatcaacat caccaggttc cagaccctgc
     2761 tggctctgca caggtcctac ctgacacctg gagactcctc ctctggctgg acagcaggag
     2821 cagcagccta ctatgtgggc tacctccaac caaggacctt cctgctgaaa tacaatgaga
     2881 atggcaccat cacagatgct gtggactgtg ccctggaccc actgtctgag accaagtgta
     2941 ccctgaaatc cttcacagtg gagaagggca tctaccagac cagcaacttc agggtccaac
     3001 caacagagag cattgtgagg tttccaaaca tcaccaacct gtgtccattt gacgaggtgt
     3061 tcaatgccac caggtttgcc tctgtctatg cctggaacag gaagaggatt agcaactgtg
     3121 tggctgacta ctctgtgctc tacaactttg cccccttctt cgccttcaag tgttatggag
     3181 tgagcccaac caaactgaat gacctgtgtt tcaccaatgt ctatgctgac tcctttgtga
     3241 ttaggggaaa tgaggtgagt cagattgccc ctggacaaac aggcaccatt gctgactaca
     3301 actacaaact gcctgatgac ttcacaggct gtgtgattgc ctggaacagc aacaagctgg
     3361 acagcaatgg tggaggcaac tacaactaca tgtacagact gttcaggaag agcaagctga
     3421 aaccatttga gagggacatc agcacagaga tttaccaggc tggcaacaca ccatgtaaag
     3481 gagtggcggg cttcaactgt tactttccac tccagtccta tggcttcaga ccaacctatg
     3541 gagtgggcca ccaaccatac agggtggtgg tgctgtcctt tgaactgctc catgcccctg
     3601 ccacagtgtg tggaccaaag aagagcacca acctggtgaa gaacaagtgt gtgaacttca
     3661 acttcaatgg actgacaggc acaggagtgc tgacagagag caacaagaag ttcctgccat
     3721 tccaacagtt tggcagggac attgctgaca ccacagatgc tgtgagggac ccacagacct
     3781 tggagattct ggacatcaca ccatgttcct ttggaggagt gtctgtgatt acacctggca
     3841 ccaacaccag caaccaggtg gctgtgctct accagggtgt gaactgtact gaggtgtctg
     3901 tggctatcca tgctgaccaa cttacaccaa cctggagggt ctacagcaca ggcagcaatg
     3961 gcttccagac cagggctggc tgtctgattg gagcagagta tgtgaacaac tcctatgagt
     4021 gtgacatccc aattggagca ggcatctgtg cctcctacca gacccagacc aggagccata
     4081 ggagggcaag gtctgtggca agccagccta tcattgccta cacaatgagt ctgggagcag
     4141 agaactctgt ggcttacagc aacaacagca ttgccatccc aaccaacttc accatctctg
     4201 tgaccacaga aattctgcct gtgagtatga ccaagacctc tgtggactgt acaatgtata
     4261 tctgtggaga cagcacagag tgtagcaacc tgctgctcca atatggctcc ttctgtaccc
     4321 aacttaagag ggctctgaca ggcattgctg tggaacagga caagaacacc caggaggtgt
     4381 ttgcccaggt gaagcagatt tacaagatac ctccaatcaa gcactttgga ggcttcaact
     4441 tcagccagat tctgcctgac ccaagcaagc caagcaagag gtccttcatt gaggacctgc
     4501 tgttcaacaa ggtgaccctg gctgatgctg gcttcatcaa gcaatatgga gactgtctgg
     4561 gagacattgc tgcaagggac ctgatttgtg cccagaagtt caatggactg accgtgctgc
     4621 ctccactgct gacagatgag atgattgccc aatacacctc tgccctgctg gctggcacca
     4681 tcacctctgg ctggaccttt ggagcaggag cagccctcca aatcccattt gctatgcaga
     4741 tggcttacag gttcaatggc attggagtga cccagaatgt gctctatgag aaccagaaac
     4801 tgattgccaa ccagttcaac tctgccattg gcaagattca gggctccctg tccagcacag
     4861 cctctgccct gggcaaactc caagatgtgg tgaaccataa tgcccaggct ctgaacaccc
     4921 tggtgaagca actttccagc aagtttggag ccatctcctc tgtgctgaat gacatcctga
     4981 gcagactgga caaggtggag gctgaggtcc agattgacag actgattaca ggcagactcc
     5041 aatccctcca aacctatgtg acccaacaac ttatcagggc tgctgagatt agggcatctg
     5101 ccaacctggc tgccaccaag atgagtgagt gtgtgctggg acaaagcaag agggtggact
     5161 tctgtggcaa gggctaccac ctgatgagtt ttccacagtc tgcccctcat ggagtggtgt
     5221 tcctgcatgt gacctatgtg cctgcccagg agaagaactt caccacagcc cctgccatct
     5281 gccatgatgg caaggctcac tttccaaggg agggagtgtt tgtgagcaat ggcacccact
     5341 ggtttgtgac ccagaggaac ttctatgaac cacagattat caccacagac aacacctttg
     5401 tgtctggcaa ctgtgatgtg gtgattggca ttgtgaacaa cacagtctat gacccactcc
     5461 aacctgaact ggactccttc aaggaggaac tggacaaata cttcaagaac cacaccagcc
     5521 ctgatgtgga cctgggagac atctctggca tcaatgcctc tgtggtgaac atccagaagg
     5581 agattgacag actgaatgag gtggctaaga acctgaatga gtccctgatt gacctccaag
     5641 aactgggcaa atatgaacaa tacatcaagt ggccatggta catctggctg ggcttcattg
     5701 ctggactgat tgccattgtg atggtgacca taatgctgtg ttgtatgacc tcctgttgtt
     5761 cctgtctgaa aggctgttgt tcctgtggct cctgttgtaa gtgactcgag ccatggaagc
     5821 ttgatatcta actgactgaa ccggtggtac caaacggggg aggctaactg aaacacggaa
     5881 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     5941 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     6001 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     6061 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     6121 ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     6181 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6241 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6301 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6361 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6421 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6481 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6541 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     6601 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6661 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6721 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6781 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6841 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     6901 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     6961 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     7021 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     7081 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     7141 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     7201 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     7261 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     7321 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     7381 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     7441 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     7501 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     7561 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     7621 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     7681 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     7741 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     7801 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7861 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7921 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7981 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8041 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8101 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8161 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8221 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8281 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8341 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8401 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8461 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8521 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8581 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8641 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8701 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8761 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8821 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8881 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8941 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9001 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9061 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9121 aaggatctca a
//
