LOCUS       sequence-437270-       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine homolog of complement 4 fused with GFP under CAG 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Phadke RA, Brack A, Fournier LA, Kruzich E, Sha M, Picard I, Johnson
            C, Stroumbakis D, Salgado M, Yeung C, Escude Velasco B, Liu YY, 
            Cruz-Martin A
  TITLE     The schizophrenia risk gene C4 induces pathological synaptic loss by
            impairing AMPAR trafficking.
  JOURNAL   Mol Psychiatry. 2025 Feb;30(2):796-809. doi: 
            10.1038/s41380-024-02701-7. Epub 2024 Sep 3.
  PUBMED    39227431
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41380-024-02701-7"; journalName: "Mol Psychiatry"; date: 
            "2025-02"; volume: "30"; issue: "2"; pages: "796-809"
COMMENT     Sequence Label: mC4-GFP
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     primer_bind     1590..1612
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1674..1693
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(7115..7117,7118..7120,7121..7834)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7115..7117,7118..7120,7121..7831)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7160..7181)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7421..7440)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7768..7789
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7921..7940)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7986..8041
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8040..8059)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8402..8418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8402..8418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8415..8437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8426..8442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8450..8456,8457..8474,8475..8480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8495..8516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8575..8770
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8621..8756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8776..8910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8813..8832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8867..8886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8978..8995)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9149..9737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9229..9248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9908..10699,10700..10768))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10531..10550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10769..10873)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggsgc gccaggcggg gcggggcggg gcggggcgag
      541 gggtggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     1321 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     1381 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     1441 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     1501 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     1561 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     1621 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1681 gctggttatt gtgctgtctc atcattttgg caaagaattc tgcagtcgac ggtaccgcgg
     1741 gcccgggatc caccggtaga agggagcaga cgtctgattt cagtcagacc agacaggtct
     1801 gacctttcct ggatcctcca gccatgcggc tcctctgggg gctggcctgg gtgttcagct
     1861 tctgtgcctc atccctgcag aagcccaggt tgctcctgtt ttccccttct gtggttaatt
     1921 tggggacccc cctgtcggtg ggggtacagc tcctggatgc ccctccagga caggaggtaa
     1981 aaggatcagt gttcctcaga aacccaaagg gtggttcctg ctccccaaag aaggacttta
     2041 agctgagctc gggagatgac tttgtgctgc tcagccttga ggtcccactg gaagatgtga
     2101 ggagctgtgg cctctttgac ctgcgcagag ccccccacat ccagctggta gctcagtctc
     2161 cgtggctaag gaacacagct ttcaaagcca cagagactca gggtgtcaac ttgctcttct
     2221 cttcccgacg aggccacatc tttgtgcaga ccgatcagcc tatctataat ccggggcagc
     2281 gggttcgtta tcgggtcttt gcactggatc aaaagatgcg cccatccact gatttcctca
     2341 ccatcacagt ggagaactcc catggcctcc gtgtactcaa gaaggagata tttacttcca
     2401 catccatctt ccaagatgcc ttaactccca tggacatctc agagcctggg acctggaaga
     2461 tctcagctag gttctcagat ggactggagt ccaataggag cacccacttt gaagtgaaga
     2521 agtatgtcct tcccaacttc gaggtgaaga ttactccttg gaagccatat atcctgatgg
     2581 tgcccagcaa cagtgatgaa atccaattag acatccaggc caggtacatc tatgggaagc
     2641 ccgtgcaggg cgtggcatac acacggtttg cgctcatgga tgagcaaggg aagaggactt
     2701 tccttcgggg cctagagacg caggccaagt tggtggaagg ccggacccac atttccatct
     2761 caaaggacca gttccaggct gccctggata aaatcaatat tggggtcaga gacctggagg
     2821 ggctgcgtct ctatgctgct acagctgtca tcgagtctcc aggaggagag atggaggagg
     2881 cagaactcac gtcctggcgc tttgtatcat ctgccttctc cttggatctc agccgcacta
     2941 agaggcatct tgtgcctggg gcccacttcc tgctgcaggc tctggtccaa gaaatgtcag
     3001 gctctgaagc ctccaacgtt cctgttaaag tctctgccac attggtgtca ggctctgact
     3061 cccaagtcct tgacattcaa cagagcacca atggaattgg ccaagtcagc atttccttcc
     3121 ccatcccacc aactgtcaca gaacttcgac tcttggtgtc tgcgggctcc ctctacccag
     3181 ccatagccag gctcaccgtg caagccccac cttcaagagg cactggcttt ctttctattg
     3241 agccactaga ccctcggtcc cctagtgtgg gggacacctt tatcctaaac cttcaacctg
     3301 tgggcatccc tgcacctacc ttctctcatt actactacat gatcatctcc agaggccaga
     3361 tcatggctat gggtcgggag ccccggaaga ctgtgacctc cgtctctgtg ttggtggacc
     3421 atcagctggc tccctcgttc tactttgtgg cttacttcta tcaccaagga cacccggtgg
     3481 ccaactctct gctcatcaac atccaatcca gggactgtga gggcaagctg caattgaagg
     3541 tggatggtgc caaggagtat cgtaatgcgg acatgatgaa gctccgaatt caaactgact
     3601 ccaaagccct ggtggcactg ggagctgtgg acacggctct gtatgctgtg ggtggtcggt
     3661 ctcacaaacc cctcgacatg agcaaggtct ttgaagtaat caacagctac aatgttggct
     3721 gtggtcctgg aggtggggat gatgcccttc aggtgttcca ggatgctggt ctggcctttt
     3781 ctgatggtga tcgactaact caaaccagag aggacctgag ctgtcccaag gagaagaaaa
     3841 gtcggcaaaa gagaaatgtt aacttccaga aggctgtcag tgagaagttg ggccagtatt
     3901 cttctccaga tgccaagcgc tgctgccaag acgggatgac gaagctgccc atgaagcgta
     3961 cctgtgagca gcgggctgcc cgtgtgcctc agcaggcctg ccgtgagccc ttcttgtcct
     4021 gttgcaagtt tgctgaggac cttcgcagga accagaccag gagccaggca caccttgccc
     4081 gaaacaacca caacatgctg caggaggaag acttgataga tgaagacgac attcttgtgc
     4141 gcacctcctt cccagagaac tggctctgga gagtggaacc tgtagacagc tccaaactgt
     4201 tgacagtgtg gcttcctgat tctatgacca catgggagat tcatggtgtg agcctgtcca
     4261 aaagcaaagg tctgtgtgta gccaagccaa ctcgtgttcg agtgttcaga aaattccacc
     4321 tgcacctgcg cctgcccatc tccatccgcc gctttgagca gtttgaatta cggcctgttc
     4381 tttacaacta tctgaatgat gatgtggctg tgagtgtcca tgtgacccca gttgaggggc
     4441 tgtgcctggc tggtggtgga atgatggccc agcaggtgac agtgccagca ggttctgccc
     4501 ggcctgtggc cttctctgtg gtacccacag ctgctgccaa cgtgcccctg aaggtggtgg
     4561 ctcgaggggt ttttgattta ggggatgctg tgtctaagat tctccaaatt gagaaggaag
     4621 gagccatcca cagagaagag ttagtctaca acctcgaccc cctaaataac ctgggtcgga
     4681 ctttggagat tcctggcagc tcggatccca acatcgtccc tgacggagac ttcagcagct
     4741 tagtcagggt tacagcctcg gaacccttgg agactatggg ttctgaaggt gccttgtccc
     4801 caggaggtgt ggcctccctc ctgaggcttc cccagggctg tgcagagcaa accatgatct
     4861 atttggctcc taccctgact gcttccaact acctggacag gacagaacag tggagcaaac
     4921 tgtcccctga aacaaaggac catgctgtgg atctgatcca aaaaggatac atgaggatcc
     4981 agcagtttcg gaagaatgat ggctcctttg gggcttggtt acaccgggac agcagcacct
     5041 ggctgactgc cttcgtgctg aagattctga gtttggccca ggaacaggtg ggcaactccc
     5101 cggagaagct gcaggagacg gctagctggc tgctggccca gcagctgggt gatggctcct
     5161 tccacgaccc atgtccagtc atccacagag caatgcaggg gggcttggtg gggtctgacg
     5221 agacagtggc actgaccgcc tttgtggtca ttgcccttca ccatgggttg gacgtcttcc
     5281 aggatgacga tgcgaagcag ctgaagaaca gagtggaagc ctccatcacc aaggcaaact
     5341 cattcttggg gcagaaggca agtgctgggc tcctgggtgc ccacgccgcc gctatcacag
     5401 cctatgccct tacgctgacc aaggcctcgg aggacctgca gaatgttgcc cacaacagcc
     5461 tgatggccat ggctgaggaa acaggggaac acctctactg gggcttagtc cttggctctc
     5521 aggacaaagt tgtgttgcgc cccacagccc cccgtagccc tacagaacct gtgccccagg
     5581 ccccagcctt gtggatcgaa accacagcct atgccctgct ccacctgctt ctgcgtgagg
     5641 gaaagggaaa aatggctgac aaggctgcat cctggctcac ccaccaggga agcttccatg
     5701 gggcattccg cagtacccag gacactgtgg tcaccctgga tgccctgtct gcctactgga
     5761 tcgcttcgca caccactgag gagaaagcac tgaacgtgac gctcagctcc atgggccgca
     5821 atgggcttaa gacccacggg ctacacttga acaaccacca agtcaagggc ctggaggagg
     5881 agctaaagtt ctccctgggc agcacaatca gtgtcaaggt ggaaggaaac agcaaaggca
     5941 ccttgaagat ccttcgtacc tacaatgtcc tggacatgaa gaacaccaca tgccaggacc
     6001 ttcagataga agtgaaggtc acaggcgctg tggaatatgc atgggatgcc aatgaagact
     6061 acgaagacta ctatgacatg ccagccgcag atgaccccag cgtccccttg cagcctgtca
     6121 cgcccctgca gctttttgag ggtcgtagga gccgccgcag gagggaggcc cccaaggtgg
     6181 ctgaagagcg ggagtccaga gttcagtaca ctgtgtgtat ctggcgaaat ggcaagctgg
     6241 ggctgtctgg catggccatc gcagacatca ccctcctaag tggattccac gccctgaggg
     6301 ctgacctgga gaagctgacc tccctctctg accgttacgt gagtcacttt gagactgacg
     6361 ggccccatgt cctgttgtac tttgactcgg tccctaccac ccgggagtgt gtgggcttcg
     6421 gagcctcgca ggaggtggtt gtgggactgg tgcagccatc cagtgctgtc ctgtatgact
     6481 actacagccc tgatcacaag tgctctgtgt tttatgctgc acccaccaag agccagctcc
     6541 tggccacact gtgctctgga gatgtatgcc agtgcgctga ggggaagtgc cctcgactgc
     6601 taaggtcact ggagcgaagg gtggaggaca aggatggcta ccggatgagg ttcgcctgct
     6661 attatccccg agtggagtat ggcttcacgg ttaaggttct tcgagaagat ggcagagctg
     6721 ccttccgtct ctttgagtcc aagatcaccc aagtcctgca tttcagaaag gacaccatgg
     6781 cctccatagg tcagacccgc aacttcctga gccgggcctc ttgccgcctt cgtttggagc
     6841 ctaacaaaga gtacttgatc atggggatgg acggggaaac cagtgacaac aagggagacc
     6901 cccagtactt gctggactca aatacctgga ttgaggagat gccttcagaa caaatgtgca
     6961 agagcacccg ccatcgggca gcctgtttcc agctcaaaga cttcctgatg gagttcagca
     7021 gccgggggtg ccaggtgggg ggggggagcg ggggggggag catggtgagc aagggcgagg
     7081 agctgttcac cggggtggtg gggggggggg gagcatggtg agcaagggcg aggagctgtt
     7141 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     7201 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     7261 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     7321 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     7381 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     7441 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     7501 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     7561 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     7621 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     7681 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     7741 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     7801 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcactcctc aggtgcaggc
     7861 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     7921 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     7981 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     8041 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     8101 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     8161 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     8221 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     8281 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     8341 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     8401 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8461 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8521 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     8581 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8641 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8701 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8761 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8821 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8881 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     8941 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9001 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9061 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9121 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9181 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9241 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9301 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9361 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9421 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9481 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9541 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9601 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9661 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9721 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9781 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9841 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9901 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9961 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10021 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10081 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10141 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10201 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10261 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10321 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10381 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10441 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10501 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10561 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10621 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10681 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10741 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10801 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10861 taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
