LOCUS       sequence-437287-       13129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e 
            adenosine deaminase and an optimized PcrA M6 helicase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Chen XD, Chen Z, Wythes G, Zhang Y, Orr BC, Sun G, Chao YK, Navarro 
            Torres A, Thao K, Vallurupalli M, Sun J, Borji M, Tkacik E, Chen H, 
            Bernstein BE, Chen F
  TITLE     Helicase-assisted continuous editing for programmable mutagenesis of
            endogenous genomes.
  JOURNAL   Science. 2024 Oct 11;386(6718):eadn5876. doi: 
            10.1126/science.adn5876. Epub 2024 Oct 11.
  PUBMED    39388570
REFERENCE   2  (bases 1 to 13129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-10-11"; volume: "386(6718):eadn5876. doi"; pages: " 
            10.1126/science.adn5876. Epub 2024 Oct 11"
COMMENT     Sequence Label: tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
FEATURES             Location/Qualifiers
     source          1..13129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2635..2949
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2643..2661
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2679..2697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2750..2768
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2786..2804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2857..2875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2922..2946
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2971..2995
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5777..5797
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(5812..5836)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5878..6388
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6307..6325
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6398..6994
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6398..6417)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6894..6914
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6995..7048
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7049..7666,7667..7672,7673..7708,7709..7711,7712..7747,
                     7748..7750,7751..7786,7787..7795)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7821..8409
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7874..7894)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8292..8303)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8412..8428)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8413..8429)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8934..9114
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9140..9157)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9146..9370
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9416..9844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9503..9522)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9713..9734
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9853..9873)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9858..10187
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10038..10173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10100..10119
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10235..10282
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10301..10675
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10805..10926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10842..10861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10896..10915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10975..10991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10975..10991)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10988..11010)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10999..11015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11023..11029,11030..11047,11048..11053))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11068..11089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11206..11223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11377..11965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11457..11476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12136..12927,12928..12996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12759..12778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12997..13101)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acagagatcc actttggccg cgaatcgata tgtcgagttt
     2641 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     2701 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     2761 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     2821 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
     2881 tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
     2941 cagatcgcct ggagaattgg ctaagcatca acaagtttgt acaaaaaaac gggatccacc
     3001 ggtcgccacc atgagttctg aggtggagtt ttcccacgag tactggatga gacatgccct
     3061 gaccctggcc aagagggcac gggatgagag ggaggtgcct gtgggagccg tgctggtgct
     3121 gaacaataga gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc
     3181 ccatgccgaa attatggccc tgagacaggg cggcctggtc atgcagaact acagactgat
     3241 tgacgccacc ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca
     3301 ctctaggatc ggccgcgtgg tgtttggcgt gaggaactca aaaagaggcg ccgcaggctc
     3361 cctgatgaac gtgctgaact accccggcat gaatcaccgc gtcgaaatta ccgagggaat
     3421 cctggcagat gaatgtgccg ccctgctgtg cgatttctat cggatgccta gacaggtgtt
     3481 caatgctcag aagaaggccc agagctccat caacagcggc agcgagactc ccgggacctc
     3541 agagtccgcc acacccgaaa gtatgaactt cctgtctgag caactgctgg cacacctgaa
     3601 caaggagcag caagaagctg tgcggaccac cgagggacct ctgctgatca tggccggcgc
     3661 tggaagcgga aaaacaagag tgctgacaca ccgcatcgcc tacctgatgg ctgagaagca
     3721 cgtggcccct tggaacatcc tggccattac ctttacaaac aaggccgcta gagagatgag
     3781 agaaagagtg cagagcctcc tgggaggcgc cgccgaggac gtgtggatca gcaccttcgc
     3841 cagcatggcc gtgcggatcc tgagaagaga tatcgacaga atcggcatca accggaactt
     3901 cagcatcctg gacccaacag accagctgag cgtgatgaaa accatcctga aagaaaagaa
     3961 catcgacccc aagaaattcg agcctagaac aatcctgggc acaatcagcg ccgccaagaa
     4021 tgaactgctg cctccagaac agtttgcgaa gcgggcctcc acctactatg agaaagtggt
     4081 gtctgacgtc taccaggagt atcagcagcg gctgctcagg tgtcacagcc ttgacttcga
     4141 tgatctgatc atgaccacaa tccagctgtt tgaccgggtc cccgacgtgc tccactacta
     4201 ccaatacaag tttcagtaca tccacatcga tgagtaccag gacacaaaca gagcccaata
     4261 caccctggtg aaaaagctgg ctgagcggtt ccagaacatc gccgccgtgg gcgacgccga
     4321 tcagtctatc tacagatggc ggggcgccga catccagaac atcctgagct tcgaaagaga
     4381 ttaccccaac gccaaagtga tcctgctgga gcaaaattac cggagcacga agcgcatcct
     4441 gcaggccgca aacgaggtga tcgagcacaa cgtgaacaga aagcctaagc ggatctggac
     4501 cgagaatcct gagggcaagc ccatcctgta ctacgaggcc atgaacgaag ccgacgaggc
     4561 ccagttcgtg gccggcagaa tcagagaggc cgtggagcgc ggcgagagaa gataccgaga
     4621 cttcgccgtg ctgtacagaa ccaatgccca gtccagagtc atggaagaga tgctgctgaa
     4681 ggccaacatc ccttaccaga tagtgggcgg cgtgaagttc tacgacagaa aggaaatcaa
     4741 ggacatcctg gcttatctgc gggtcatcgc aaacccagac gacgactgca gcctgctgag
     4801 aattatcaac gttcctaaaa gaggcatcgg agcctctacc atcgacaagc tggtccggta
     4861 cgccgccgac cacgagctga gcctgttcga ggccctgggc gagctggaga tgatcggtct
     4921 tggcgccaag gccgctgggg ccctggccgc cttcagaagc cagctggaac agtggacaca
     4981 gctgcaggag tacgtgagcg tgaccgaact ggtggaagag gtgctggaca agtctggcta
     5041 ccgggaaatg ctgaaggccg aaagaaccat cgaagcccag agcagactgg agaacctgga
     5101 tgagttcctg agcgtgacca agcatttcga aaacgtgtcc gacgacaaga gcctgatcgc
     5161 cttcctgacc gatctggctc tgatcagcga cctggacgag ctggatggca ccgagcaggc
     5221 cgccgagggc gacgccgtga tgctgatgac cctgcacgcc gccaagggcc tggaattccc
     5281 cgtggtcttt ctgataggca tggaagaagg catcttccct cacaatagaa gcctggagga
     5341 tgacgatgag atggaagagg agagaagact ggcctacgtg ggcatcacca gagctgaaga
     5401 ggaactggtg ctgaccagcg cccagatgcg gaccctgttc ggcaacattc agatggaccc
     5461 tcctagccgg ttccttaacg agatccccgc ccatctgctt gagacagcca gcagacggca
     5521 ggccggagct tccaggcctg ccgtgagcag accccaggcc tctggcgctg tgggcagctg
     5581 gaaggtgggc gatcgggcaa atcacagaaa gtggggcatc ggaaccgtgg tttccgtgcg
     5641 gggcggcgga gatgatcagg aactggacat cgcttttcca tctcctatcg gcattaagag
     5701 actgctagct aaattcgccc ctatcgagaa ggtgtctggt ggttctaaaa gaaccgccga
     5761 cggcagcgaa ttcgagccca agaagaagag gaaagtctaa agcggccgct tccccagctt
     5821 tcttgtacaa agtggtttag taatgaaccg gtccaccacc accaccacta aggatccggg
     5881 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     5941 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     6001 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     6061 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     6121 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     6181 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     6241 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     6301 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     6361 tgaccgaatc accgacctct ctccccagca attcaccatg accgagtaca agcccacggt
     6421 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6481 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6541 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6601 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6661 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6721 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6781 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6841 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6901 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6961 gcgcacctgg tgcatgaccc gcaagcccgg tgccgaaggt agaggttctc tcctcacttg
     7021 tggtgatgtt gaagaaaacc ctggtccaat gtctagactg gacaagagca aagtcataaa
     7081 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     7141 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     7201 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     7261 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     7321 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     7381 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     7441 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     7501 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     7561 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     7621 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     7681 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     7741 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaaagaat
     7801 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     7861 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7921 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7981 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     8041 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     8101 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     8161 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     8221 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8281 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8341 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8401 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     8461 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     8521 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     8581 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8641 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     8701 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     8761 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     8821 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     8881 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     8941 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9001 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9061 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     9121 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     9181 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     9241 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     9301 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     9361 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     9421 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9481 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9541 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9601 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9661 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9721 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9781 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9841 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     9901 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     9961 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    10021 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    10081 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
    10141 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
    10201 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
    10261 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
    10321 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
    10381 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
    10441 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
    10501 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
    10561 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
    10621 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
    10681 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    10741 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    10801 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10921 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
    10981 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    11041 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    11101 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    11161 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    11221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    11281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    11821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    12001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    12061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    12121 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12181 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12241 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12301 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12361 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12421 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12481 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12541 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12601 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    12661 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12721 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    13021 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13081 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
