LOCUS       sequence-437290-        4933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase with minimal Albumin promoter with a 380 nt, 
            -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested 
            pPromALB_FireflyLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Chang TY, Waxman DJ
  TITLE     HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver 
            in vivo.
  JOURNAL   BMC Genomics. 2024 Dec 24;25(1):1240. doi: 
            10.1186/s12864-024-11162-9.
  PUBMED    39716078
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2024-12-24"; volume: "25(1):1240. doi"; pages: " 
            10.1186/s12864-024-11162-9"
COMMENT     Sequence Label: pGYL17_pPromALB_enhALB_FireflyLuc
FEATURES             Location/Qualifiers
     source          1..4933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1169..1290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1180..1199)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1234..1253
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1538..1555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1709..2297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1789..1808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2497..3288,3289..3357))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3462..3510
                     /note="synthetic polyadenylation signal"
     misc_feature    3524..3615
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3564..3583
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      4400..4409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4406..4933,1..1125)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg
       61 aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt
      121 gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct
      181 atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg
      241 atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc
      301 ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct
      361 aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg
      421 gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc
      481 cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac
      541 gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg
      601 gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg
      661 atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc
      721 tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac
      781 cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc
      841 atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat
      901 gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag
      961 gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt
     1021 gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc
     1081 gagattctca ttaaggccaa gaagggcggc aagatcgccg tgtaataatt ctagagtcgg
     1141 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     1201 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1261 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     1321 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     1381 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     1441 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     1501 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1561 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1621 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1681 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1741 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1801 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1861 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1921 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1981 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2041 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2101 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2161 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2221 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2461 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     2521 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     2581 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     2641 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     2701 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     2761 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     2821 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     2881 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     2941 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     3001 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     3061 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     3121 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     3181 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     3241 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     3301 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     3361 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     3421 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     3481 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     3541 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     3601 ccagaacatt tctctggcct aactggccgg taccgattac attacacatt ctgcaagcat
     3661 agcacagagc aatgttctac tttaattact ttcattttct tgtatcctca cagcctagaa
     3721 aataacctgc gttacagcat ccactcagta tcccttgagc atgaggtgac actacttaac
     3781 atagggacga gatggtactt tgtgtctcct gctctgtcag cagggcactg tacttgctga
     3841 taccagggaa tgtttgttct taaataccat cattccggac gtgtttgcct tggccagttt
     3901 tccatgtaca tgcagaaaga agtttggact gatcaataca gtcctctgcc tttaaagcaa
     3961 taggaaaagg ccaacttgtc tacgtttagt atgtggctgt agaaagggta tagactcgag
     4021 gatatcaaga tctggcctcg gcggccaagc tttgctcaaa tgggagacaa agagattaag
     4081 ctcttatgta aaatttgctg ttttacataa ctttaatgaa tggacaaagt cttgtgcatg
     4141 ggggtggggg tggggttaga ggggaacagc tccagatggc aaacatacgc aagggattta
     4201 gtcaaacaac tttttggcaa agatggtatg attttgtaat ggggtaggaa ccaatgaaat
     4261 gcgaggtaag tatggttaat aatctacagt tattggttaa agaagtatat tagagcgagt
     4321 ctttctgcac acagatcacc ttcctatcaa ccccactagc ctctggcaaa aagcttggca
     4381 atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga agggcccagc
     4441 gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg
     4501 ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac
     4561 ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct
     4621 gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt
     4681 gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg
     4741 cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg
     4801 gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat
     4861 ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt
     4921 gccacccggc ttc
//
