LOCUS       sequence-437293-        5469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase with mouse liver DNase hypersensitive site # 
            50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Chang TY, Waxman DJ
  TITLE     HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver 
            in vivo.
  JOURNAL   BMC Genomics. 2024 Dec 24;25(1):1240. doi: 
            10.1186/s12864-024-11162-9.
  PUBMED    39716078
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2024-12-24"; volume: "25(1):1240. doi"; pages: " 
            10.1186/s12864-024-11162-9"
COMMENT     Sequence Label: pTYC4_pPromALB_enh50358_FireflyLuc
FEATURES             Location/Qualifiers
     source          1..5469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1321..1330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1327..2979
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3023..3144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3034..3053)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3088..3107
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3392..3409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3563..4151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3643..3662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4351..5142,5143..5211))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5316..5364
                     /note="synthetic polyadenylation signal"
     misc_feature    5378..5469
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5418..5437
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccg aaacatactc ctctaaacat cccactctga gtcctctttt
       61 tcacttctta aaaacccttc aagtccaatt tgggcagcaa cagtctattg tctggcgagg
      121 actaagtgga ttatgctgtg taaaagatac catgctttcc catcatgttc ctcagggcta
      181 gaatgaggat ctgagccgac cagttaaaga gtaatgttgt gggttcataa aagtccaagg
      241 tggcattttg ccaagttcat atgtgtgcga aggtcaggtg tactctgttg gtcctctctc
      301 atggggtcac aagagtgtat actgtctcta tctgacctcc ccacctctgt ttcagccatc
      361 tccacccaga ccagcactat cagcaatagt ttgcctctga aagctttcac ttgcttaaac
      421 tcccttactt gaaagaaccc attctacagc ctggtccctt accagctact taccctttag
      481 aacctgtggt gttctaaatg aaacactcat acgtaaagtg taaaagccac acagaacaga
      541 aaacgtacaa tgttggtctt tctgggtctg ggttacgtca tcctagatga tcatttctag
      601 tttcacagtt acctggatgt ttcgcaattt aattttttaa cagctgaata atatttccct
      661 gtgtaaatga aacacatttt cattatgggt tcatcacttg atgtgcatct tggctagttc
      721 cgatttccat atgttaccag tagagcagca agggacatgg atgagcaagt gtctgtgtag
      781 taaaatggag agtccttctg gtacatgtcc aagaatggtg tagctggagt cttgtggtag
      841 atctactttc agcttgttga agaaccgtca cagtgattcc catagtggct agaccagttt
      901 gtatttctat cagctgtgaa taagtgcttc cattcctcga ggatatcaag atctggcctc
      961 ggcggccaag ctttgctcaa atgggagaca aagagattaa gctcttatgt aaaatttgct
     1021 gttttacata actttaatga atggacaaag tcttgtgcat gggggtgggg gtggggttag
     1081 aggggaacag ctccagatgg caaacatacg caagggattt agtcaaacaa ctttttggca
     1141 aagatggtat gattttgtaa tggggtagga accaatgaaa tgcgaggtaa gtatggttaa
     1201 taatctacag ttattggtta aagaagtata ttagagcgag tctttctgca cacagatcac
     1261 cttcctatca accccactag cctctggcaa aaagcttggc aatccggtac tgttggtaaa
     1321 gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
     1381 gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc
     1441 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
     1501 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
     1561 gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
     1621 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
     1681 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
     1741 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     1801 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     1861 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     1921 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     1981 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc
     2041 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     2101 ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa
     2161 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     2221 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     2281 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     2341 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     2401 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     2461 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     2521 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     2581 cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     2641 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     2701 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     2761 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     2821 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     2881 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
     2941 ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat aattctagag tcggggcggc
     3001 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3061 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3121 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3181 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     3241 gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     3301 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     3361 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3961 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4321 gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat
     4381 ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac
     4441 tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg
     4501 ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag
     4561 tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt
     4621 aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt
     4681 atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt
     4741 cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt
     4801 cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct
     4861 taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt
     4921 ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac
     4981 cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa
     5041 agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag
     5101 ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca
     5161 aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct
     5221 ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag
     5281 taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac
     5341 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     5401 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     5461 catttctct
//
