LOCUS       sequence-437294-        4213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Renilla luciferase with minimal Albumin promoter with a 380 nt, 
            -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pGL4.70 
            (Promega).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Chang TY, Waxman DJ
  TITLE     HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver 
            in vivo.
  JOURNAL   BMC Genomics. 2024 Dec 24;25(1):1240. doi: 
            10.1186/s12864-024-11162-9.
  PUBMED    39716078
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2024-12-24"; volume: "25(1):1240. doi"; pages: " 
            10.1186/s12864-024-11162-9"
COMMENT     Sequence Label: pGYL18_pPromALB_enhALB_RenillaLuc
FEATURES             Location/Qualifiers
     source          1..4213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      785..794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             791..1726
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1767..1888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1778..1797)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1832..1851
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2136..2153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2307..2895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2387..2406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3095..3886,3887..3955))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4060..4108
                     /note="synthetic polyadenylation signal"
     misc_feature    4122..4213
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4162..4181
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccg attacattac acattctgca agcatagcac agagcaatgt
       61 tctactttaa ttactttcat tttcttgtat cctcacagcc tagaaaataa cctgcgttac
      121 agcatccact cagtatccct tgagcatgag gtgacactac ttaacatagg gacgagatgg
      181 tactttgtgt ctcctgctct gtcagcaggg cactgtactt gctgatacca gggaatgttt
      241 gttcttaaat accatcattc cggacgtgtt tgccttggcc agttttccat gtacatgcag
      301 aaagaagttt ggactgatca atacagtcct ctgcctttaa agcaatagga aaaggccaac
      361 ttgtctacgt ttagtatgtg gctgtagaaa gggtatagac tcgaggatat caagatctgg
      421 cctcggcggc caagctttgc tcaaatggga gacaaagaga ttaagctctt atgtaaaatt
      481 tgctgtttta cataacttta atgaatggac aaagtcttgt gcatgggggt gggggtgggg
      541 ttagagggga acagctccag atggcaaaca tacgcaaggg atttagtcaa acaacttttt
      601 ggcaaagatg gtatgatttt gtaatggggt aggaaccaat gaaatgcgag gtaagtatgg
      661 ttaataatct acagttattg gttaaagaag tatattagag cgagtctttc tgcacacaga
      721 tcaccttcct atcaacccca ctagcctctg gcaaaaagct tggcaatccg gtactgttgg
      781 taaagccacc atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg
      841 gcctcagtgg tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta
      901 tgattccgag aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag
      961 ctacctgtgg aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga
     1021 tctgatcgga atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca
     1081 ctacaagtac ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt
     1141 gggccacgac tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat
     1201 caaggccatc gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc
     1261 tgacatcgag gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga
     1321 gaataacttc ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga
     1381 ggagttcgct gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct
     1441 ctcctggcct cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt
     1501 ccgcaactac aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc
     1561 cgaccctggg ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga
     1621 gttcgtgaag gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa
     1681 gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag cagtaattct agagtcgggg
     1741 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     1801 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1861 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1921 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     1981 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     2041 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     2101 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2161 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2221 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3061 gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac
     3121 cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga
     3181 tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc
     3241 cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa
     3301 gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta
     3361 gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg
     3421 tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc
     3481 gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg
     3541 ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt
     3601 ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt
     3661 cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca
     3721 acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc
     3781 ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac
     3841 ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca
     3901 agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg
     3961 tcctttttca atattattga agcatttatc agggttacta gtacgtctct caaggataag
     4021 taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca
     4081 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     4141 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     4201 agaacatttc tct
//
