LOCUS       sequence-437299-        6798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 1 for expression PzPKS2 in plants 
            (N.benthamiana, BY2 cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Palkina KA, Karataeva TA, Perfilov MM, Fakhranurova LI, Markina NM, 
            Somermeyer LG, Garcia-Perez E, Vazquez-Vilar M, Rodriguez-Rodriguez 
            M, Vazquez-Vilriales V, Shakhova ES, Mitiouchkina T, Belozerova OA, 
            Kovalchuk SI, Alekberova A, Malyshevskaia AK, Bugaeva EN, Guglya EB,
            Balakireva A, Sytov N, Bezlikhotnova A, Boldyreva DI, Babenko VV, 
            Kondrashov FA, Choob VV, Orzaez D, Yampolsky IV, Mishin AS, 
            Sarkisyan KS
  TITLE     A hybrid pathway for self-sustained luminescence.
  JOURNAL   Sci Adv. 2024 Mar 8;10(10):eadk1992. doi: 10.1126/sciadv.adk1992. 
            Epub 2024 Mar 8.
  PUBMED    38457503
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-03-8"; volume: "10(10):eadk1992. doi"; pages: " 
            10.1126/sciadv.adk1992. Epub 2024 Mar 8"
COMMENT     Sequence Label: pNK7183
FEATURES             Location/Qualifiers
     source          1..6798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     302..321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(540..644)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      731..1362
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    1454..1478
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1664..2009
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1952..1972
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      3298..4005
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4054..4078
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(4234..5382)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(5718..6306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5798..5817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6477..6798,1..470,471..539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
       61 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
      121 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
      181 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
      241 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
      301 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
      361 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
      421 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
      481 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
      541 ctcttccttt ttcaatatta ttgaagcatt tatcaaggtt attgtctcat gagcggatac
      601 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacgaa ttggccagcg
      661 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
      721 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
      781 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
      841 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
      901 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
      961 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
     1021 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
     1081 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
     1141 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
     1201 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
     1261 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
     1321 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcgcaaagga gatcctgatc
     1381 tgactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     1441 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
     1501 ataacacatt gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtga
     1561 agacaagcaa gaattcaagc ttggaggtca acatggtgga gcacgacact ctggtctact
     1621 ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa
     1681 ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
     1741 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
     1801 tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     1861 tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct
     1921 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     1981 aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca
     2041 attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa
     2101 tggccccatc tgtggaagaa atcaggaagg cccagagagc tcagggcccg gccactgtcc
     2161 tcgccatcgg caccgccacc ccacccaact gcgtctatca gaaggactat ccggactact
     2221 acttccgtgt cactaactca gagcacatga ccgaattgaa ggagaagttc aggagaatgt
     2281 gcgacaaatc aatgatcgag aagcgttaca tgcttttgac cgaggatctt ctcaaggaga
     2341 accccagcat gtgcgcttac atgggatcct cccttgacgc aagacaggac ttggtcgtcg
     2401 ctgaggtccc aaggctcggc aaagaggccg cacagaaggc catcaaggag tggggccaac
     2461 ccaagtctaa gatcacccac gtcatcatgt gcaccacttc aggtgtcgac atgcctggcg
     2521 cagactacca gctcactaag cttctcggcc ttcgtccttc cgtcaagcga ttcatgatgt
     2581 accagcaagg ttgcttcgct ggtggaaccg ttcttcgact cgctaaggac gtggctgaga
     2641 acaacaaggg agcacgagtc ctcgtcgttt gctcagagat caccgccatc tgtttccgtg
     2701 gcccaacgga cacccacctg gactccatgg tgggacaagc cttattcgga gatggttcag
     2761 gagccgtcat catcggagcc gatccagact taaccgtgga gaggccaatc ttcgagctgg
     2821 tctggaccgc ccagaccatc ctcccagact ccgagggagc catcgacggt caccttagag
     2881 aagtcgggtt gactctccat ctcctcaagg acgttccagg gcttatctca aagaacatca
     2941 ccaaggcact cgtggaggcc tttaacccac ttggaatctc tgactggaac gagcttttct
     3001 gggtcgcaca ccccggtgga cccgctatcc ttgaccaggt tgaggagaaa ctcgctctca
     3061 agcctgagaa gatgaaggct acaaggcaag tgcttaatga ctacgggaac atgtcaagcg
     3121 cttgtgtgct gtttatctta gatgagatga ggaagaagtc tttggagaac ggtcattcta
     3181 ctacaggtga aggattggaa tggggtgttc tctttgggtt tggacctggg cttacggttg
     3241 agactgtcgt gctgcacagt gtccctgttg taaactcagg tagttaacta gcttgtcctg
     3301 ctttaatgag atatgcgaga agcctatgat cgcatgatat ttgctttcaa ttctgttgtg
     3361 cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt
     3421 ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt
     3481 actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata tattgtgctg
     3541 aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt gcgttttatt
     3601 attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag actgattaca
     3661 taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca caccgagcgg
     3721 cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca tgggtgagat
     3781 tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc attcaacccg
     3841 gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag catttttttg
     3901 gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac aaatcgttgg
     3961 gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggaccg ctactattgt
     4021 cttctgcacg aagtggttta aactatcagt gtttgacagg atatattggc gggtaaacct
     4081 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     4141 cgttcgtcca tttgtatgtg catgccaacc acagggttcc ccagatcagg cgctggctgc
     4201 tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc
     4261 attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac
     4321 ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc
     4381 cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt
     4441 cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag
     4501 cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag
     4561 gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa
     4621 gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc
     4681 attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc
     4741 gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc
     4801 ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt
     4861 gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc
     4921 cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag
     4981 ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct
     5041 gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg
     5101 cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcaagac gacgcgaacg
     5161 ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc
     5221 gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc
     5281 acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat
     5341 cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat
     5401 tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc ctggtgcctt
     5461 ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc
     5521 cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa
     5581 atacttgccg tgggcctcgg cctgagagcc aaaacacttg atgcggaaga agtcggtgcg
     5641 ctcctgcttg tcgccggcat cgttgcgcca catctaggat ctgccaggaa ccgtaaaaag
     5701 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5761 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5821 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5881 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5941 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6001 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6061 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6121 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6181 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6241 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6301 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6361 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6421 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6481 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6541 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6601 gctgcaatga taccgcgaga accacgctca ccggctccag atttatcagc aataaaccag
     6661 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6721 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6781 gttgccattg ctacaggc
//
