LOCUS       sequence-437301-        2676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 vector carrying terminator ORCA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Balakireva AV, Karataeva TA, Karampelias M, Mitiouchkina TY, 
            Machacek J, Shakhova ES, Perfilov MM, Belozerova OA, Palkina KA, 
            Drazna N, Vondrakova Z, Fleiss A, Fakhranurova LI, Markina NM, 
            Morozov VV, Bugaeva EN, Delnova GM, Choob VV, Yampolsky IV, Petrasek
            J, Mishin AS, Sarkisyan KS
  TITLE     Non-invasive imaging of salicylic and jasmonic acid activities in 
            planta
  JOURNAL   bioRxiv 2025.02.17.636591; doi: 
            https://doi.org/10.1101/2025.02.17.636591
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.17.636591; doi: https://doi.org/10.1101/2025.02.17.636591"
COMMENT     Sequence Label: M6667
FEATURES             Location/Qualifiers
     source          1..2676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(7..798)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1020..1124)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1192..1210
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1753..1770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1924..2512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2004..2023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgacagttat ttgccgacta ccttggtgat ctcgcctttt acgtaatgga caaattcttc
       61 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
      121 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
      181 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
      241 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
      301 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      361 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      421 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
      481 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
      541 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
      601 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
      661 gctgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
      721 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
      781 gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac
      841 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
      901 tgcccgaggc atagactgta ccccaaaaaa aaacagtcat aacaagccat gaaaaccgcc
      961 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     1021 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     1081 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1141 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1201 atcacgaggc cctttcgtcc actgaagagc cacttcgtgg tctcaagcgt ctagattacc
     1261 gaattagatt tatctatacc cctattgatt tttcttaata taattttatt ttttaaaaaa
     1321 tgaagagatt gtaattgtat ttgggtcttc gaaactcgtg actgtctagc tgatcgaaga
     1381 ccaatttaat ttaattaatg ctttggtaac ctcacaccgt gggtagcttg ctcatctact
     1441 tagttaaaaa taataataaa aaaattgtgt aattcatcat caataaattc ataagcatta
     1501 aaatgatttt gtgaactttt ttggatataa aattttgtac attccaaaga tttaaaaaag
     1561 aacatcgatg aagttaataa ttttacaatg agggattatc gggcttaaca aaaaccacgt
     1621 aataatctct catttcacag aattttttta atcctccact tttaataatt aaaagctgag
     1681 accacagagt gattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2641 taaatcaatc taaagtatat atgagtaaac ttggtc
//
