LOCUS       sequence-437306-        9214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expressing an RNA containing your sequence of interest adjacent 
            to two MS2 sites from an RNA polymerase III promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Bernstein DS, Buter N, Stumpf C, Wickens M
  TITLE     Analyzing mRNA-protein complexes using a yeast three-hybrid system.
  JOURNAL   Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4.
  PUBMED    12054889
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Methods"; date: "2002-02"; volume: "26"; issue: "2"; pages: 
            "123-41"
COMMENT     Sequence Label: pIIIA/MS2-1
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      131..1473
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    463..510
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1500..1604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1605..1673,1674..2465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1823..1842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2636..3224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3125..3144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3378..3395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3512..3533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3548..3553,3554..3571,3572..3578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3586..3602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3591..3613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3610..3626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3610..3626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3645..3665
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3647..3665
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3702..3718
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3724..4130
                     /gene="RPR1"
                     /label=RPR1 promoter
                     /note="promoter for the S. cerevisiae RNase P RNA gene"
     misc_RNA        4169..4187
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4208..4226
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     terminator      4285..4347
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     CDS             complement(5012..6727)
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     promoter        complement(6728..7103)
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     primer_bind     complement(7165..7184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7166..7184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7194..7211)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7194..7210)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7203..7225)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7351..7806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7438..7457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7648..7669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7937..8740)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8741..8956)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8980..8999)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
       61 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      121 ttcttagtat gatccaatat caaaggaaat gatagcattg aaggatgaga ctaatccaat
      181 tgaggagtgg cagcatatag aacagctaaa gggtagtgct gaaggaagca tacgataccc
      241 cgcatggaat gggataatat cacaggaggt actagactac ctttcatcct acataaatag
      301 acgcatataa gtacgcattt aagcataaac acgcactatg ccgttcttct catgtatata
      361 tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc
      421 tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag
      481 ttcctattct ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg
      541 aagacgcact ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc
      601 gaataccgct tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat
      661 atccctatat aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct
      721 ctacattttt tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac
      781 tattcataga gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat
      841 agagacaaaa tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta
      901 tcactttctg ttcacaaagt atgcgcaatc cacatcggta tagaatataa tcggggatgc
      961 ctttatcttg aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc gggaagtgga
     1021 gtcaggcttt ttttatggaa gagaaaatag acaccaaagt agccttcttc taaccttaac
     1081 ggacctacag tgcaaaaagt tatcaagaga ctgcattata gagcgcacaa aggagaaaaa
     1141 aagtaatcta agatgctttg ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca
     1201 aaaaagaagt atagattctt tgttggtaaa atagcgctct cgcgttgcat ttctgttctg
     1261 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt
     1321 ttacaaaaat gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg
     1381 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt
     1441 ttgttctaca aaatgaagca cagatgcttc gttcaggtgg cacttttcgg ggaaatgtgc
     1501 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1561 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1621 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1681 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1741 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1801 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1861 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1921 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1981 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2041 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2101 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2161 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2221 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2281 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2341 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2401 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2461 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2521 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2761 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     3601 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     3661 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatccg
     3721 ggggatctgc caattgaaca taacatggta gttacatata ctagtaatat ggttcggcac
     3781 acattaaaag tataaaaact atctgaatta cgaattacat atattggtca taaaaatcaa
     3841 tcaatcatcg tgtgttttat atgtctctta tctaagtata agaatatcca tagttaatat
     3901 tcacttacgc taccttttaa cctgtaatca ttgtcaacag gatatgttaa cgacccacat
     3961 tgataaacgc tagtatttct ttttcctctt cttattggcc ggctgtctct atactcccct
     4021 atagtctgtt tcttttcgtt tcgattgttt tacgtttgag gcctcgtggc gcacatggta
     4081 cgctgtggtg ctcgcggctg ggaacgaaac tctgggagct gcgattggca gaattccagc
     4141 ttgcatgcct gcaggtcgac tctagaaaac atgaggatca cccatgtctg caggtcgact
     4201 ctagaaaaca tgaggatcac ccatgtctgc aggtcgactc tagaggatcg cccgggggat
     4261 ccactagttc tagccggaat tcccccatat ccaacttcca atttaatctt tcttttttaa
     4321 ttttcactta tttgcgatac agaaagaaaa aagcgatagt aactattgaa ttttgtttgg
     4381 atttggttag attagatatg gtttctcttt atatttacat gctaaaaatg ggctacacca
     4441 gagatacata attagatata tatacgccag tacaccttat cggcccaagc cttgtcccaa
     4501 ggcagcgttt tgttcttgga aacgctgccc tacacgttcg ctatgcttca agaacttttc
     4561 tgagcacttc atgatgcatg tttgttcctt attggttagc tttgatgttg tgaagtcatt
     4621 gacacagtct gtgaaacatc tttctaccag attagagtac aaacgcatga aatccttcat
     4681 ttgcttttgt tccactactt tttggaactc ttgttgttct ttggagttca atgcgtccat
     4741 ctttacagtc ctgtcttatt gttcttgatt tgtgccccgt aaaatactgt tacttggttc
     4801 tggcgaggta ttggatagtt cctttttata aaggccatga agctttgcag aggctagtgg
     4861 atcttatgta tgaaattctt aaaaaaggac acctgtaagc gttgatttct atgtatgaag
     4921 tccacatttg atgtaatcat aacaaagcct aaaaaatagg tatatcattt tataattatt
     4981 tgctgtacaa gtatatcaat aaacttatat attacttgtt ttctagataa gcttcgtaac
     5041 cgacagtttc taacttttgt gctttgacaa gaacttcttc ttcttgcttt aataaaaact
     5101 gttccatttt cgttgtataa cttgaatcat aagcgccaag cagtctgaca gccaacagcg
     5161 cagcgttcgt actattatta atagcgacgg tagctactgg aacacctcta ggcatttgca
     5221 caattgaatg taaagaatct actccatcta gacaagaacc ttttacgggc acaccgatga
     5281 caggaagtgg tgtcattgca gccaccatac ctggcaagtg agcagcccca ccagctccag
     5341 cgataattgt tttaattcca cgcttgcttg cggaaatagc atatgctgac atcctatgtg
     5401 gagttctatg agcagagact attgtcactt caaatggaac gccaaaatct tttaaaaccg
     5461 cacatgcggc agacattacc ggcaagtcag agtctgatcc catgatgatt ccaaccaatg
     5521 gtttgaccat tgcttccaag tccaactttt gagcgacaga gattttgatt ggaatatcag
     5581 ttctacctgt aatgtagttc agcctttgtt cacattccgc catactggag gcaataatat
     5641 ttatgtgacc tacttttctg ttaggtctag actcttttcc atataagtac actgaggaac
     5701 ctggagtcgc caatgctctt tcgcaagttt ctagctcttt atcttttgta tgtttgtctc
     5761 caagaacatt tagcataatg gcgttcgttg taatggtgga gaaagatgtg aaattctttg
     5821 gcattggcaa atccaatatt gatctcaaat gagcttcaaa ttgagaagtg acgcaagcat
     5881 caatggtata atgtccagag ttgtgaggcc ttggggcaat ttcgttaata agcaattccc
     5941 ctgtttctaa atagaacatt tccacaccaa atataccaca accgggaaaa gatttgattg
     6001 cattttctgc caacaacttc gccttaagtt gaacggagtc cggaactcta gcaggcgcat
     6061 aacataagtc acaaatattg tccttgtgga tagtctctac aattgggtaa gaaaacacta
     6121 aaccgttaac agacctcaca atcatgactg ctaattcttt agtaaatggt gcccattttt
     6181 cggcgtacaa aggacgatcc ttcagtactt ccaaagcttc cggaatcatt tccttattct
     6241 ttacaacgaa gttacctctt ccatcgtatg ccaaagtcct cgacttcaag acgaatggaa
     6301 aacccaaatc tcttccaaca ttcaataggg acgtctcact ggcttgttcc acaggaacac
     6361 tttgggtaac tgctatacca tttttgatta aatgctcttt ttgaatatat ttgtcttgta
     6421 tcaatctgat tgtttctgga gaagggtaaa tttttaattt gggatgtttt acttgaagat
     6481 tctttagtgt aggaacatca acatgctcaa tctcaatcgt tagcacatca catttttcag
     6541 ctagtttttc gatatcaaga ggattggaaa aggagccatt aacgtggtca ttggagttgc
     6601 ttatttgttt ggcaggagaa ttttcagcat ctagtattac cgtcttaatg ttgagcctgt
     6661 ttgctgcctc aacaatcata cgtcccaatt gtccccctcc taatatacca actgttctag
     6721 aatccatact tgattgtttt gtccgatttt cttgtttttc ttgattgtta tagtaggatg
     6781 tacttagaag agagatccaa cgattttacg caccaattta tacatgaaat gctccataat
     6841 attgtccatt tagttcttaa taaaaggtca gcaagagtca atcacttagt attacccggt
     6901 tcgtagccat gcaacaagag tcatttgtca gcatagctgt aataatcaat catgacgtaa
     6961 gaaatgtatc ataattaaaa gttgttaaag atgtcagtgt tatgttggtg ttacaaaatt
     7021 ctcggcttct cactaatatt taatatctct taaattttat ctgtctttga ttcttttaag
     7081 aaaagttatg tattattcaa gaattaattc agatccccgg ccggggatcc tctagagtcg
     7141 agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7201 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7261 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7321 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     7381 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7441 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7501 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7561 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7621 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7681 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7741 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7801 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg
     7861 gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta
     7921 cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg
     7981 cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc
     8041 caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac
     8101 ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac
     8161 cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc
     8221 tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg
     8281 acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct
     8341 tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt
     8401 ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa
     8461 attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa
     8521 ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt
     8581 tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag
     8641 cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag
     8701 gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc
     8761 aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac
     8821 tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt
     8881 cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa
     8941 aaaatgatga attgaaaagg tggtatggtg cactctcagt acaatctgct ctgatgccgc
     9001 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     9061 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     9121 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     9181 ataggttaat gtcatgataa taatggtttc ttag
//
