LOCUS       sequence-437308-        8479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Dewey RA, Morrissey G, Cowsill CM, Stone D, Bolognani F, Dodd NJ, 
            Southgate TD, Klatzmann D, Lassmann H, Castro MG, Loewenstein PR
  TITLE     Chronic brain inflammation and persistent herpes simplex virus 1 
            thymidine kinase expression in survivors of syngeneic glioma treated
            by adenovirus-mediated gene therapy: implications for clinical 
            trials.
  JOURNAL   Nat Med. 1999 Nov . 5(11):1256-63.
  PUBMED    10545991
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            1999 Nov . 5(11):1256-63."
COMMENT     Sequence Label: pAL119-TK
FEATURES             Location/Qualifiers
     source          1..8479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        577..780
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     731..751
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     777..801
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             876..2006
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             6030..6221
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6215..6237)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6323..6463
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6378..6397
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6478..6495)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6649..7237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6729..6748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7408..8199,8200..8268))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8031..8050
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8269..8373)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8441..8459
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccatcatcat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgccggatg tggtaaaagt gacgtttttg
      181 gtgtgcgccg gtgtatacgg gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccaag taatatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataattct gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
      421 cgggtcaaag ttggcgtttt attattatag tcagctctag actcgagaag cttgcatgcc
      481 tgcaggtcaa ttccctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      601 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      781 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      841 agacaccggg accgatccag cctggggatc ccgacatggc ttcgtacccc tgccatcaac
      901 acgcgtctgc gttcgaccag gctgcgcgtt ctcgcggcca tagcaaccga cgtacggcgt
      961 tgcgccctcg ccggcagcaa gaagccacgg aagtccgcct ggagcagaaa atgcccacgc
     1021 tactgcgggt ttatatagac ggtcctcacg ggatggggaa aaccaccacc acgcaactgc
     1081 tggtggccct gggttcgcgc gacgatatcg tctacgtacc cgagccgatg acttactggc
     1141 aggtgctggg ggcttccgag acaatcgcga acatctacac cacacaacac cgcctcgacc
     1201 agggtgagat atcggccggg gacgcggcgg tggtaatgac aagcgcccag ataacaatgg
     1261 gcatgcctta tgccgtgacc gacgccgttc tggctcctca tatcgggggg gaggctggga
     1321 gctcacatgc cccgcccccg gccctcaccc tcatcttcga ccgccatccc atcgccgccc
     1381 tcctgtgcta cccggccgcg cgatacctta tgggcagcat gaccccccag gccgtgctgg
     1441 cgttcgtggc cctcatcccg ccgaccttgc ccggcacaaa catcgtgttg ggggcccttc
     1501 cggaggacag acacatcgac cgcctggcca aacgccagcg ccccggcgag cggcttgacc
     1561 tggctatgct ggccgcgatt cgccgcgttt acgggctgct tgccaatacg gtgcggtatc
     1621 tgcagggcgg cgggtcgtgg cgggaggatt ggggacagct ttcggggacg gccgtgccgc
     1681 cccagggtgc cgagccccag agcaacgcgg gcccacgacc ccatatcggg gacacgttat
     1741 ttaccctgtt tcgggccccc gagttgctgg cccccaacgg cgacctgtac aacgtgtttg
     1801 cctgggcctt ggacgtcttg gccaaacgcc tccgtcccat gcacgtcttt atcctggatt
     1861 acgaccaatc gcccgccggc tgccgggacg ccctgctgca acttacctcc gggatggtcc
     1921 agacccacgt caccaccccc ggctccatac cgacgatctg cgacctggcg cgcacgtttg
     1981 cccgggagat gggggaggct aactgaggat cccacgtcac tattgtatac tctatattat
     2041 actctatgtt atactctgta atcctactca ataaacgtgt cacgcctgtg aaaccgtact
     2101 aagtctcccg tgtcttctta tcaccatcag gtgacatcct cgcccaggct gtcaatcatg
     2161 ccggtatcga ttccagtagc accggcccca cgctgacaac ccactcttgc agcgttagca
     2221 gcgcccctct taacaagccg acccccacca gcgtcgcggt tactaacact cctctccccg
     2281 acctgcagcc caagcttctc gagtctagag tggaaggtgc tgaggtacga tgagacccgc
     2341 accaggtgca gaccctgcga gtgtggcggt aaacatatta ggaaccagcc tgtgatgctg
     2401 gatgtgaccg aggagctgag gcccgatcac ttggtgctgg cctgcacccg cgctgagttt
     2461 ggctctagcg atgaagatac agattgaggt actgaaatgt gtgggcgtgg cttaagggtg
     2521 ggaaagaata tataaggtgg gggtcttatg tagttttgta tctgttttgc agcagccgcc
     2581 gccgccatga gcaccaactc gtttgatgga agcattgtga gctcatattt gacaacgcgc
     2641 atgcccccat gggccggggt gcgtcagaat gtgatgggct ccagcattga tggtcgcccc
     2701 gtcctgcccg caaactctac taccttgacc tacgagaccg tgtctggaac gccgttggag
     2761 actgcagcct ccgccgccgc ttcagccgct gcagccaccg cccgcgggat tgtgactgac
     2821 tttgctttcc tgagcccgct tgcaagcagt gcagcttccc gttcatccgc ccgcgatgac
     2881 aagttgacgg ctcttttggc acaattggat tctttgaccc gggaacttaa tgtcgtttct
     2941 cagcagctgt tggatctgcg ccagcaggtt tctgccctga aggcttcctc ccctcccaat
     3001 gcggtttaaa acataaataa aaaaccagac tctgtttgga tttggatcaa gcaagtgtct
     3061 tgctgtcttt atttaggggt tttgcgcgcg cggtaggccc gggaccagcg gtctcggtcg
     3121 ttgagggtcc tgtgtatttt ttccaggacg tggtaaaggt gactctggat gttcagatac
     3181 atgggcataa gcccgtctct ggggtggagg tagcaccact gcagagcttc atgctgcggg
     3241 gtggtgttgt agatgatcca gtcgtagcag gagcgctggg cgtggtgcct aaaaatgtct
     3301 ttcagtagca agctgattgc caggggcagg cccttggtgt aagtgtttac aaagcggtta
     3361 agctgggatg ggtgcatacg tggggatatg agatgcatct tggactgtat ttttaggttg
     3421 gctatgttcc cagccatatc cctccgggga ttcatgttgt gcagaaccac cagcacagtg
     3481 tatccggtgc acttgggaaa tttgtcatgt agcttagaag gaaatgcgtg gaagaacttg
     3541 gagacgccct tgtgacctcc aagattttcc atgcattcgt ccataatgat ggcaatgggc
     3601 ccacgggcgg cggcctgggc gaagatattt ctgggatcac taacgtcata gttgtgttcc
     3661 aggatgagat cgtcataggc catttttaca aagcgcgggc ggagggtgcc agactgcggt
     3721 ataatggttc catccggccc aggggcgtag ttaccctcac agatttgcat ttcccacgct
     3781 ttgagttcag atggggggat catgtctacc tgcggggcga tgaagaaaac ggtttccggg
     3841 gtaggggaga tcagctggga agaaagcagg ttcctgagca gctgcgactt accgcagccg
     3901 gtgggcccgt aaatcacacc tattaccggc tgcaactggt agttaagaga gctgcagctg
     3961 ccgtcatccc tgagcagggg ggccacttcg ttaagcatgt ccctgactcg catgttttcc
     4021 ctgaccaaat ccgccagaag gcgctcgccg cccagcgata gcagttcttg caaggaagca
     4081 aagtttttca acggtttgag accgtccgcc gtaggcatgc ttttgagcgt ttgaccaagc
     4141 agttccaggc ggtcccacag ctcggtcacc tgctctacgg catctcgatc cagcatatct
     4201 cctcgtttcg cgggttgggg cggctttcgc tgtacggcag tagtcggtgc tcgtccagac
     4261 gggccagggt catgtctttc cacgggcgca gggtcctcgt cagcgtagtc tgggtcacgg
     4321 tgaaggggtg cgctccgggc tgcgcgctgg ccagggtgcg cttgaggctg gtcctgctgg
     4381 tgctgaagcg ctgccggtct tcgccctgcg cgtcggccag gtagcatttg accatggtgt
     4441 catagtccag cccctccgcg gcgtggccct tggcgcgcag cttgcccttg gaggaggcgc
     4501 cgcacgaggg gcagtgcaga cttttgaggg cgtagagctt gggcgcgaga aataccgatt
     4561 ccggggagta ggcatccgcg ccgcaggccc cgcagacggt ctcgcattcc acgagccagg
     4621 tgagctctgg ccgttcgggg tcaaaaacca ggtttccccc atgctttttg atgcgtttct
     4681 tacctctggt ttccatgagc cggtgtccac gctcggtgac gaaaaggctg tccgtgtccc
     4741 cgtatacaga cttgagaggc ctgtcctcga ccgatgccct tgagagcctt caacccagtc
     4801 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     4861 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     4921 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     4981 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     5041 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     5101 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     5161 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     5221 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc
     5281 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     5341 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     5401 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
     5461 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
     5521 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     5581 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     5641 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     5701 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
     5761 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     5821 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
     5881 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
     5941 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     6001 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
     6061 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     6121 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     6181 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6241 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6301 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     6361 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     6421 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     6481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7081 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     7741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     8041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8401 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     8461 ctttcgtctt caagaattc
//
