LOCUS       sequence-437309-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expressing an RNA containing your sequence of interest adjacent 
            to two MS2 sites from an RNA polymerase III promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Bernstein DS, Buter N, Stumpf C, Wickens M
  TITLE     Analyzing mRNA-protein complexes using a yeast three-hybrid system.
  JOURNAL   Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4.
  PUBMED    12054889
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Methods"; date: "2002-02"; volume: "26"; issue: "2"; pages: 
            "123-41"
COMMENT     Sequence Label: pIIIA/MS2-2
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2047..2422
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             2423..4138
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     terminator      complement(4803..4865)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(4898..4916)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(4937..4955)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(5020..5426)
                     /gene="RPR1"
                     /label=RPR1 promoter
                     /note="promoter for the S. cerevisiae RNase P RNA gene"
     primer_bind     complement(5432..5448)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5485..5505)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5485..5503)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5524..5540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5524..5540)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5537..5559)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5548..5564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5572..5578,5579..5596,5597..5602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5617..5638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5755..5772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5926..6514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6006..6025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6685..7476,7477..7545))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7308..7327
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7546..7650)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7677..9019
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8640..8687)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9060..9078
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgg gccccccctc gactctagag gatccccggc cggggatctg
     2041 aattaattct tgaataatac ataacttttc ttaaaagaat caaagacaga taaaatttaa
     2101 gagatattaa atattagtga gaagccgaga attttgtaac accaacataa cactgacatc
     2161 tttaacaact tttaattatg atacatttct tacgtcatga ttgattatta cagctatgct
     2221 gacaaatgac tcttgttgca tggctacgaa ccgggtaata ctaagtgatt gactcttgct
     2281 gaccttttat taagaactaa atggacaata ttatggagca tttcatgtat aaattggtgc
     2341 gtaaaatcgt tggatctctc ttctaagtac atcctactat aacaatcaag aaaaacaaga
     2401 aaatcggaca aaacaatcaa gtatggattc tagaacagtt ggtatattag gagggggaca
     2461 attgggacgt atgattgttg aggcagcaaa caggctcaac attaagacgg taatactaga
     2521 tgctgaaaat tctcctgcca aacaaataag caactccaat gaccacgtta atggctcctt
     2581 ttccaatcct cttgatatcg aaaaactagc tgaaaaatgt gatgtgctaa cgattgagat
     2641 tgagcatgtt gatgttccta cactaaagaa tcttcaagta aaacatccca aattaaaaat
     2701 ttacccttct ccagaaacaa tcagattgat acaagacaaa tatattcaaa aagagcattt
     2761 aatcaaaaat ggtatagcag ttacccaaag tgttcctgtg gaacaagcca gtgagacgtc
     2821 cctattgaat gttggaagag atttgggttt tccattcgtc ttgaagtcga ggactttggc
     2881 atacgatgga agaggtaact tcgttgtaaa gaataaggaa atgattccgg aagctttgga
     2941 agtactgaag gatcgtcctt tgtacgccga aaaatgggca ccatttacta aagaattagc
     3001 agtcatgatt gtgaggtctg ttaacggttt agtgttttct tacccaattg tagagactat
     3061 ccacaaggac aatatttgtg acttatgtta tgcgcctgct agagttccgg actccgttca
     3121 acttaaggcg aagttgttgg cagaaaatgc aatcaaatct tttcccggtt gtggtatatt
     3181 tggtgtggaa atgttctatt tagaaacagg ggaattgctt attaacgaaa ttgccccaag
     3241 gcctcacaac tctggacatt ataccattga tgcttgcgtc acttctcaat ttgaagctca
     3301 tttgagatca atattggatt tgccaatgcc aaagaatttc acatctttct ccaccattac
     3361 aacgaacgcc attatgctaa atgttcttgg agacaaacat acaaaagata aagagctaga
     3421 aacttgcgaa agagcattgg cgactccagg ttcctcagtg tacttatatg gaaaagagtc
     3481 tagacctaac agaaaagtag gtcacataaa tattattgcc tccagtatgg cggaatgtga
     3541 acaaaggctg aactacatta caggtagaac tgatattcca atcaaaatct ctgtcgctca
     3601 aaagttggac ttggaagcaa tggtcaaacc attggttgga atcatcatgg gatcagactc
     3661 tgacttgccg gtaatgtctg ccgcatgtgc ggttttaaaa gattttggcg ttccatttga
     3721 agtgacaata gtctctgctc atagaactcc acataggatg tcagcatatg ctatttccgc
     3781 aagcaagcgt ggaattaaaa caattatcgc tggagctggt ggggctgctc acttgccagg
     3841 tatggtggct gcaatgacac cacttcctgt catcggtgtg cccgtaaaag gttcttgtct
     3901 agatggagta gattctttac attcaattgt gcaaatgcct agaggtgttc cagtagctac
     3961 cgtcgctatt aataatagta cgaacgctgc gctgttggct gtcagactgc ttggcgctta
     4021 tgattcaagt tatacaacga aaatggaaca gtttttatta aagcaagaag aagaagttct
     4081 tgtcaaagca caaaagttag aaactgtcgg ttacgaagct tatctagaaa acaagtaata
     4141 tataagttta ttgatatact tgtacagcaa ataattataa aatgatatac ctatttttta
     4201 ggctttgtta tgattacatc aaatgtggac ttcatacata gaaatcaacg cttacaggtg
     4261 tcctttttta agaatttcat acataagatc cactagcctc tgcaaagctt catggccttt
     4321 ataaaaagga actatccaat acctcgccag aaccaagtaa cagtatttta cggggcacaa
     4381 atcaagaaca ataagacagg actgtaaaga tggacgcatt gaactccaaa gaacaacaag
     4441 agttccaaaa agtagtggaa caaaagcaaa tgaaggattt catgcgtttg tactctaatc
     4501 tggtagaaag atgtttcaca gactgtgtca atgacttcac aacatcaaag ctaaccaata
     4561 aggaacaaac atgcatcatg aagtgctcag aaaagttctt gaagcatagc gaacgtgtag
     4621 ggcagcgttt ccaagaacaa aacgctgcct tgggacaagg cttgggccga taaggtgtac
     4681 tggcgtatat atatctaatt atgtatctct ggtgtagccc atttttagca tgtaaatata
     4741 aagagaaacc atatctaatc taaccaaatc caaacaaaat tcaatagtta ctatcgcttt
     4801 tttctttctg tatcgcaaat aagtgaaaat taaaaaagaa agattaaatt ggaagttgga
     4861 tatgggggaa ttccgatcct ctagagtcga cctgcagaca tgggtgatcc tcatgttttc
     4921 tagagtcgac ctgcagacat gggtgatcct catgttttct agagtcgacc tgcaggcatg
     4981 caagctgccc gggggatcca ctagttctag ccggaattct gccaatcgca gctcccagag
     5041 tttcgttccc agccgcgagc accacagcgt accatgtgcg ccacgaggcc tcaaacgtaa
     5101 aacaatcgaa acgaaaagaa acagactata ggggagtata gagacagccg gccaataaga
     5161 agaggaaaaa gaaatactag cgtttatcaa tgtgggtcgt taacatatcc tgttgacaat
     5221 gattacaggt taaaaggtag cgtaagtgaa tattaactat ggatattctt atacttagat
     5281 aagagacata taaaacacac gatgattgat tgatttttat gaccaatata tgtaattcgt
     5341 aattcagata gtttttatac ttttaatgtg tgccgaacca tattactagt atatgtaact
     5401 accatgttat gttcaattgg cagatccccc ggatccacta gttctagagc ggccgccacc
     5461 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     5521 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5581 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6361 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaacg
     7681 aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa
     7741 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc
     7801 aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt
     7861 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt
     7921 ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta
     7981 tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt
     8041 ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc
     8101 tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga
     8161 agctgcgggt gcattttttc aagataaagg catccccgat tatattctat accgatgtgg
     8221 attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa
     8281 ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt
     8341 cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt
     8401 aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg
     8461 aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca aagagatact
     8521 tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt
     8581 gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg
     8641 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga
     8701 aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg
     8761 cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg
     8821 tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta
     8881 cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct
     8941 ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca
     9001 tttcctttga tattggatca tactaagaaa ccattattat catgacatta acctataaaa
     9061 ataggcgtat cacgaggccc tttcgtc
//
