LOCUS       sequence-43732-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion between the Lck domain and cytosolic GCaMP5G. 
            This construct targets GCaMP5G to the plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Akerboom J, Chen TW, Wardill TJ, Tian L, Marvin JS, Mutlu S, 
            Calderon NC, Esposti F, Borghuis BG, Sun XR, Gordus A, Orger MB, 
            Portugues R, Engert F, Macklin JJ, Filosa A, Aggarwal A, Kerr RA, 
            Takagi R, Kracun S, Shigetomi E, Khakh BS, Baier H, Lagnado L, Wang 
            SS, Bargmann CI, Kimmel BE, Jayaraman V, Svoboda K, Kim DS, 
            Schreiter ER, Looger LL
  TITLE     Optimization of a GCaMP calcium indicator for neural activity 
            imaging.
  JOURNAL   J Neurosci. 2012 Oct 3;32(40):13819-40. doi: 
            10.1523/JNEUROSCI.2601-12.2012.
  PUBMED    23035093
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2601-12"; journalName: "J Neurosci"; date: 
            "2012-10-3- 3"; volume: "32"; issue: "40"; pages: "13819-40"
COMMENT     Sequence Label: pN1 Lck-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             177..194
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             198..230
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(234..242,243..257)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             285..341
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     554..575
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(681..702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gttagtgaac cgtcagatcc gctagcgcta ccggactcag atctgccacc atgggctgtg
       61 gctgcagctc aaaccctgaa gatgactgga tggagaacat tgacgtgtgt gagaactgcc
      121 attatccccc gccgaagctt cgaattgatc caccggatct cgccaccatg ggttctcatc
      181 atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt
      241 acgacgatga cgataaggat ctcgccacca tggtcgactc atcacgtcgt aagtggaata
      301 agacaggtca cgcagtcaga gctataggtc ggctgagctc actcgagaac gtctatatca
      361 aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
      421 acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg
      481 tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa actttcgaaa gaccccaacg
      541 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      601 tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt
      661 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      721 gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      781 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      841 tgcagtgctt cagccgctac cccgaccaca tga
//
