LOCUS       sequence-437323-        2146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_RiboJ57-MCD2
FEATURES             Location/Qualifiers
     source          1..2146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(183..277)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(298..957)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     872..891
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1119..1137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1141..1184)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1306..1363
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1387..1404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1558..2146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1638..1657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
      181 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      241 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      301 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      361 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      421 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      481 gtttaaatca aaactggtga aactcaccca gggattggct gacacgaaaa acatattctc
      541 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      601 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      661 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      721 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      781 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      841 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      901 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      961 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1021 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     1081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     1141 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttcggtctc agtacagaag
     1201 tcaattaatg tgcttttaat tctgatgagt cggtgacgac gaaacttcct ctacaaataa
     1261 ttttgtttaa tcttaatcat gctaaggatc gtttcataag agacctccgg caaaaaaggg
     1321 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca
     1381 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1561 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1981 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctacca gcggtggttt
     2101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaa
//
