LOCUS       sequence-437326-        3167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 promoter part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-P_nahR-PSalTTC
FEATURES             Location/Qualifiers
     source          1..3167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(183..277)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(298..957)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     872..891
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1119..1137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1152..1191)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      2327..2384
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2408..2425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2579..3167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2659..2678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
      181 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      241 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      301 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      361 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      421 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      481 gtttaaatca aaactggtga aactcaccca gggattggct gacacgaaaa acatattctc
      541 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      601 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      661 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      721 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      781 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      841 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      901 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      961 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1021 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     1081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     1141 ggtctcacta ttccaaaaaa aaaggctcca aaaggagcct ttaattgtat ctcaatccgt
     1201 aaacaggtca aacatcagtt gccgcaacca aatattggct aggtccttgt ggtacttcgc
     1261 atgccagaac atgttgatgg ctatttcagg caagacgact gggtgcggca aggcgcttag
     1321 gccgaagggc tctacgcagc agtcggctaa acatatcggc acagtggcga gcagatcggt
     1381 gcgctggagg atgtggccaa cggcggcgaa gtgcggcact tccagacgga tgtcgcgccg
     1441 gatgccgacc cgtgtcatgt acgtgtccac ctcgccgtgg ccggtgccag cggcgatgac
     1501 acgcacgtgg ccgtaggaac agaagcgctc cagagtcagg ggttcgcggg tgactggatg
     1561 gtccttgcga cataggcaca cgtagtgatt ctggagcagc cggcgctgaa agaagccagt
     1621 ttgcagattg ggaagcaggc ccacggccaa gtccacggtt ccgttctgca aggcctgcat
     1681 caggctcatc gaactgtcgc gcaccgtact gatcacgcaa ttgggggcct ggtgagccag
     1741 cgcatccatc agccgcggca tgaagtagat ctcgccaatg tcggtcatgg ccagggtgaa
     1801 ggtacgctcg ctggtcagcg gatcgaagct ttcatggtgc tgtagggcgt tgcgcagtgc
     1861 gtgcatggcc gaagtgacgt gctcggccag atgcgcggca tagggtgtgg gttccattcc
     1921 ctgatgtgtg cgcacgaaga gtgggtcctg tagcgaggtg cgcaggcgtt tcagcgcatt
     1981 gctcacggca ggctgggtca ggcccaggtt ctccgcagtg acagagacgc gtctgtcgac
     2041 cagcaactgg ttgaacacca ccagcaggtt taaatcaagg tcacgcagtt ccatattcaa
     2101 cccccatgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcacc
     2161 attcgatggc gcgccgcggg gcctcgcttg ggttattgct ggtgcccggc cgggcgcaat
     2221 attcatgttg atgatttatt atatatcgag tggtgtattt atttatattg tttgctccgt
     2281 taccgttatt aacgtacaga gacccatgcc gtctcattag ctgcagtccg gcaaaaaagg
     2341 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
     2401 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2461 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2521 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2581 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2641 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2701 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2761 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2821 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2881 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2941 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3001 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3061 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3121 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaa
//
