LOCUS       sequence-437333-        2904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-StayGold-9
FEATURES             Location/Qualifiers
     source          1..2904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(784..802)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        870..974
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1196..1987
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2158..2746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2647..2666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2900..2904,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggcctcta ctccattcaa attccagttg
      121 aaaggaacta ttaatggtaa gtctttcact gtggaaggtg aaggagaggg aaattctcac
      181 gaaggtagtc ataagggaaa gtacgtttgt acaagcggaa aactgcctat gtcatgggca
      241 gctcttggta catcttttgg ttatggaatg aagtactaca ctaaatatcc ttctggtctt
      301 aagaattggt ttcatgaagt gatgccagag ggcttcactt atgataggca tatccaatat
      361 aagggagatg gatcaattca cgctaagcac caacacttta tgaagaatgg aacttatcat
      421 aacatagttg aatttactgg tcaagatttt aaagagaact ctcctgttct tacaggagat
      481 atgaatgttt cacttcctaa tgaggtgcag catatcccaa gagatgatgg agtggaatgt
      541 ccagtgacac tgctttaccc tttgttgtct gataagtcaa agtgtgttga ggcctaccag
      601 aacactatca ttaagccact gcataatcaa ccagctcctg atgttcccta tcactggatt
      661 aggaagcaat atactcagtc taaggatgat acagaagaga gagatcatat cattcagagc
      721 gaaactcttg aggcacattt gtgagctttg agaccacgaa gtggctcttc agtggacgaa
      781 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      841 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      901 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      961 gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1021 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg gggtacagtc
     1081 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     1141 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     1201 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     1261 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     1321 gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     1381 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     1441 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     1501 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     1561 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     1621 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     1681 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     1741 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     1801 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     1861 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     1921 cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg
     1981 caaataactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2041 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2101 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2161 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2221 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2281 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2341 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2401 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2461 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2521 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2821 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2881 cagccgaacg accgagcgca gcga
//
