LOCUS       sequence-437367-       14797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14797)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 14797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-ASH1L
FEATURES             Location/Qualifiers
     source          1..14797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        129..508
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        509..712
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     663..683
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     709..733
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(837..839,840..842,843..1553)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(882..903)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1143..1162)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1490..1511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1569..1593
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10516..10540)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10548..10589
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(10629..10648)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10673..10721
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10923..11351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11010..11029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11220..11241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11360..11380)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11365..11694
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11545..11680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11607..11626
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11742..11789
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11808..12206
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    12364..12485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12401..12420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12455..12474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12534..12550)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12534..12550)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12547..12569)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12558..12574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12582..12588,12589..12606,12607..12612))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12627..12648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12765..12782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12936..13524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13016..13035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13695..14486,14487..14555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14318..14337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14556..14660)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14735..14754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
       61 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
      121 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      181 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      241 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      301 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      361 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      421 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      481 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      541 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      601 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      661 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      721 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      781 ggaccgatcc agcctccgga ctctagagga tccctaccgg tgatatcctc gagaccatgg
      841 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      901 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      961 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1021 tgaccacctt cacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     1081 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1141 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1201 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1261 tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca
     1321 tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1381 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1441 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1501 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggctcga
     1561 gcccatcaac aagtttgtac aaaaaagcag aagggccgtc aaggcccacc atggacccta
     1621 gaaatactgc tatgttagga ttgggttctg attccgaagg tttttcaaga aagagtcctt
     1681 ctgccatcag tactggcaca ttggtcagta agagagaagt agagctagaa aaaaacacaa
     1741 aggaggaaga ggaccttcgc aaacggaatc gagaaagaaa catcgaagct gggaaagatg
     1801 atggtttgac tgatgcacag caacagtttt cagtgaaaga aacaaacttt tcagagggaa
     1861 atttaaaatt gaaaattggc ctccaggcta agagaactaa aaaacctcca aagaacttgg
     1921 agaactatgt atgtcgacct gccataaaaa caactattaa gcacccaagg aaagcactta
     1981 aaagtggaaa gatgacggat gaaaagaatg aacactgtcc ttcaaaacga gacccttcaa
     2041 agttgtacaa gaaagcagat gatgttgcag ccattgaatg ccagtctgaa gaagtcatcc
     2101 gtcttcattc acagggagaa aacaatcctt tgtctaagaa gctgtctcca gtacactcag
     2161 aaatggcaga ttatattaat gcaacgccat ctactcttct tggtagccgg gatcctgatt
     2221 taaaggacag agcattactt aatggaggaa ctagtgtaac agaaaagttg gcacagctga
     2281 ttgctacctg tcctccttcc aagtcttcca agacaaaacc gaagaagtta ggaactggca
     2341 ctacagcagg attggttagc aaggatttga tcaggaaagc aggtgttggc tctgtagctg
     2401 gaataataca taaggactta ataaaaaagc caaccatcag cacagcagtt ggattggtaa
     2461 ctaaagatcc tgggaaaaag ccagtgttta atgcagcagt aggattggtc aataaggact
     2521 ctgtgaaaaa actgggaact ggcactacag cggtattcat taataaaaac ttaggcaaaa
     2581 agccaggaac tatcactaca gtaggactgc taagcaaaga ttcaggaaag aagctaggaa
     2641 ttggtattgt tccaggttta gtgcataaag agtctggcaa gaagttagga cttggcactg
     2701 tggttggact ggttaataaa gatttgggaa agaaattggg ttctactgtt ggcctagtgg
     2761 ccaaggactg tgcaaagaag attgtagcaa gttcagcaat gggattggtt aataaggaca
     2821 ttggaaagaa actaatgagt tgtcctttgg caggtctgat cagtaaagat gccataaacc
     2881 ttaaagccga agcactgctc cccactcagg aaccgcttaa ggcttcttgt agtacaaaca
     2941 tcaataatca ggaaagtcag gaactttctg aatccctgaa agatagtgcc accagcaaaa
     3001 cttttgaaaa gaatgttgta cggcagaata aagaaagcat attggaaaag ttctcagtac
     3061 gaaaagaaat cattaatttg gagaaagaaa tgtttaatga aggaacatgc attcagcaag
     3121 acagtttctc atccagtgaa aagggatctt atgaaacctc aaagcatgaa aagcagcctc
     3181 ctgtatattg cacttctccg gactttaaaa tgggaggtgc ttctgatgta tctaccgcta
     3241 aatccccatt cagtgcagta ggagaaagca atctcccttc cccatcacct actgtatctg
     3301 ttaatccttt aaccagaagt ccccctgaaa cttcttcaca gttggctcct aatccattac
     3361 ttttaagttc tactacagaa ctaatcgaag aaatttctga atctgttgga aagaaccagt
     3421 ttacttctga aagtacccac ttgaacgttg gtcataggtc agttggtcat agtataagta
     3481 ttgaatgtaa agggattgat aaagaggtaa atgattcaaa aactacccat atagatattc
     3541 caagaataag ctcttccctt ggaaaaaagc caagtttgac ttctgaatcc agcattcata
     3601 ctattactcc ttcagttgtt aacttcacta gtttatttag taataagcct tttttaaaac
     3661 tgggtgcagt atctgcatca gacaaacact gccaagttgc tgaaagccta agtactagtt
     3721 tgcagtccaa accattaaaa aaaagaaaag gaagaaaacc tcggtggact aaagtggtgg
     3781 caagaagcac atgccggtct ccaaaagggc tagaattaga aagatcagag ctttttaaaa
     3841 acgtttcatg tagctcacta tcaaatagta attctgagcc agccaagttt atgaaaaaca
     3901 ttggaccccc ttcatttgta gatcatgact tccttaaacg ccgattgcca aagttgagca
     3961 aatccacagc tccatctctt gctctcttag ctgatagtga aaaaccatct cataagtctt
     4021 ttgctactca caaactatcc tccagtatgt gtgtctctag tgaccttttg tctgatattt
     4081 ataagcccaa aagaggaagg cctaaatcta aggagatgcc tcaactggaa gggccaccta
     4141 aaaggacttt aaaaatccct gcttctaaag tgttttcttt acagtctaag gaagaacaag
     4201 aacccccaat tttacagcca gaaattgaaa tcccttcctt caaacaaggt ctgtctgtgt
     4261 ctccttttcc aaaaaagaga ggcaggccta agaggcaaat gaggtcacca gtcaagatga
     4321 agccacctgt actgtcagtg gctccatttg ttgccactga aagtccaagc aagctagaat
     4381 ctgaaagtga caaccataga agtagcagtg atttctttga gagcgaggat caacttcagg
     4441 atccagatga cctagatgac agtcataggc caagtgtctg tagtatgagt gaccttgaga
     4501 tggaaccaga taaaaaaatt accaagagaa acaatggaca attaatgaaa acaattatcc
     4561 gcaaaataaa taaaatgaag actttaaaga gaaagaaact gttgaatcag attctttcaa
     4621 gttctgtaga atcaagtaat aaagggaaag tgcaatccaa actccataat acggtatcaa
     4681 gtcttgctgc cacatttggc tctaaattgg gccaacagat aaatgtcagc aagaaaggaa
     4741 ccatttatat aggaaagaga agaggtcgca aaccaaaaac tgtcttaaat ggtattcttt
     4801 ctggtagtcc tactagcctt gctgttcttg agcaaacagc tcaacaggca gctgggtcag
     4861 cattaggaca gattcttccc ccattactgc cttcatctgc tagtagttct gagattcttc
     4921 catcacctat ttgctctcag tcttctggga ctagtggagg tcagagccct gtaagtagtg
     4981 atgcaggttt tgttgaaccc agttcagtgc catatttgca tttacactcc agacagggca
     5041 gtatgattca gactcttgca atgaagaagg cctcaaaggg gaggaggcgg ttatctcctc
     5101 ctactttgtt gccaaattct ccttcgcact tgagtgaact cacatctcta aaagaagcta
     5161 ctccttcccc aatcagtgag tctcatagtg atgagaccat tcccagtgat agtggaattg
     5221 gaacagataa taacagcaca tcagacaggg cagagaaatt ttgtgggcaa aaaaagagga
     5281 ggcattcttt tgagcatgtt tctctgattc cccctgaaac ctctacagtg ctaagcagtc
     5341 ttaaagaaaa acataaacac aaatgtaagc gcaggaatca tgattacctc agctatgaca
     5401 agatgaaaag gcagaaacga aaacggaaaa agaaatatcc ccagcttcga aatagacagg
     5461 atccagactt tattgcagag ctggaggaac taataagtcg cctaagtgaa attcggatca
     5521 ctcatcgaag tcatcatttt atcccccgag atcttctgcc aactatcttt cgaatcaact
     5581 ttaatagttt ctatacacat ccttctttcc ccttagaccc tttgcactac attcgaaaac
     5641 ctgacttaaa aaagaaaaga gggagacccc ctaagatgag ggaggcaatg gctgaaatgc
     5701 cttttatgca cagccttagt tttcctcttt ctagtactgg attctatcca tcttatggta
     5761 tgccttactc tccttcaccc cttacagctg ctcccatagg attaggttac tatggaaggt
     5821 atcctcccac tctttatcca cctcctccat ctccttcttt caccacgcca cttccacctc
     5881 cttcctatat gcatgctggt catttacttc tcaatcctgc caaataccat aagaaaaagc
     5941 ataagctact tcgacaggag gcctttctta caaccagcag gactcccctc ctttccatga
     6001 gtacctaccc cagtgttcct cctgagatgg cctatggttg gatggttgag cacaaacaca
     6061 ggcaccgtca caaacacaga gaacaccgtt cttctgaaca accccaggtt tctatggaca
     6121 ctggctcttc ccgatctgtc ctggaatctt tgaagcgcta tagatttgga aaggatgctg
     6181 ttggagagcg atataagcat aaggaaaagc accgttgtca catgtcctgc cctcatctct
     6241 ctccttcaaa aagcttaata aacagagagg aacagtgggt ccaccgagag ccttcagaat
     6301 ctagtccatt ggccttggga ttgcagacac ctttacagat tgactgttca gaaagttctc
     6361 caagcttatc ccttggagga ttcactccca actctgagcc agccagcagt gatgaacata
     6421 caaacctttt cacaagtgca ataggcagct gcagagtttc aaaccctaac tccagtggcc
     6481 ggaagaaatt aactgacagc cctggactct tttctgcaca ggacacttca ctaaatcggc
     6541 ttcacagaaa ggagtcactg ccttctaacg aaagggcagt acagactttg gcaggctccc
     6601 agccaacctc tgataaaccc tcccagcggc catcagagag cacaaattgt agccctaccc
     6661 ggaaaaggtc ttcatctgag agtacttctt caacagtaaa cggagttccc tctcgaagtc
     6721 caagattagt tgcttctggg gatgactctg tggatagtct gctgcagcgg atggtacaaa
     6781 atgaggacca agagcccatg gagaaaagta ttgatgctgt gattgcaact gcctctgcac
     6841 caccttcttc cagtccaggc cgtagccaca gcaaggaccg aaccctggga aaaccagaca
     6901 gccttttagt gcctgcagtc acaagtgact cttgcaataa tagcatctca ctcctatctg
     6961 aaaagttgac aagcagctgt tccccccatc atatcaagag aagtgtagtg gaagctatgc
     7021 aacgccaagc tcggaaaatg tgcaattacg acaaaatctt ggccacaaag aaaaacctag
     7081 accatgtcaa taaaatctta aaagccaaaa aacttcaaag gcaggccagg acagggaata
     7141 actttgtgaa acgtaggcca ggtcgacctc ggaaatgtcc ccttcaggct gtcgtatcaa
     7201 tgcaagcatt ccaggctgct cagtttgtca acccagaatt gaacagagac gaggaaggag
     7261 cagcactgca cctcagtcct gacacagtta cagatgtaat tgaggctgtt gttcagagtg
     7321 taaatctgaa cccagaacat aaaaaggggt tgaagagaaa aggttggcta ttggaagaac
     7381 agaccagaaa aaagcagaag ccattaccag aggaagaaga gcaagagaat aataaaagct
     7441 ttaatgaagc accagttgag attcccagtc cttctgaaac cccagctaaa ccttctgaac
     7501 ctgaaagtac cttgcagcct gtgctttctc tcatcccaag ggaaaagaag cccccacgtc
     7561 ccccaaagaa gaagtatcag aaagcagggc tgtattctga cgtttacaaa actacagacc
     7621 caaagagtcg attgatccaa ttaaagaaag agaagctgga gtatactcca ggagagcatg
     7681 aatatggatt atttccagcg cccattcatg ttggaaagta tctaagacaa aagagaattg
     7741 acttccagct tccttatgat atcctttggc agtggaaaca caatcagcta tacaaaaagc
     7801 cagatgtccc actatataag aaaattcgtt caaatgtcta cgttgatgtc aaaccccttt
     7861 ctggttacga agctaccacc tgtaactgta agaagccaga tgatgacacc aggaagggct
     7921 gtgttgatga ctgcctcaat agaatgatct ttgctgagtg ttcccccaac acttgcccat
     7981 gtggcgagca atgctgtaac cagaggatac agaggcatga atgggtgcaa tgtctagaac
     8041 gatttcgagc tgaggaaaaa ggttggggaa tcagaaccaa agagccccta aaagctgggc
     8101 agttcatcat tgaataccta ggggaggtcg tcagtgaaca ggagttcagg aacaggatga
     8161 ttgagcagta tcataatcac agtgaccact actgcctgaa cctggatagt gggatggtga
     8221 ttgacagtta ccgcatggga aatgaggccc gattcatcaa ccatagctgt gacccaaatt
     8281 gtgaaatgca gaaatggtct gttaatggag tataccggat tggactctat gctcttaaag
     8341 acatgccagc tgggactgaa ctcacttatg attataactt tcattccttc aatgtggaaa
     8401 aacagcaact ttgtaagtgt ggctttgaga aatgtcgagg aatcatcgga ggcaagagtc
     8461 agcgtgtgaa tggactcacc agcagcaaaa acagccagcc catggccaca cacaaaaaat
     8521 ctggacggtc aaaagagaag agaaagtcta agcacaagct gaagaaaagg agaggccatc
     8581 tctctgagga acccagtgaa aatatcaaca ccccaactag attgaccccc caattacaga
     8641 tgaagccaat gtccaatcgt gaaaggaact ttgtgttaaa gcatcatgta ttcttggtcc
     8701 gaaactggga gaagattcgt caaaaacagg aggaagtaaa gcacaccagt gataatattc
     8761 actcagcatc attatatacc cgttggaatg ggatctgccg agatgatggg aatatcaagt
     8821 ctgatgtctt catgacccag ttctctgccc tgcagacagc tcgatctgtt cgaacaagac
     8881 ggttggcagc tgcagaggaa aatattgaag tggctcgggc agcccgccta gcccagatct
     8941 tcaaagaaat ttgtgatggt atcatctctt ataaagattc ttcccggcaa gcactggcag
     9001 ctccactttt gaaccttccc ccaaagaaaa agaatgctga ttattatgag aagatctctg
     9061 atcccctaga tcttatcacc atagagaagc agatcctcac tggttactat aagacagtgg
     9121 aagcttttga tgctgacatg ctcaaagtct ttcggaatgc tgagaagtac tatgggcgta
     9181 aatccccagt tgggagagat gtttgtcgtc tacgaaaggc ctattacaat gcccggcatg
     9241 aggcatcagc ccagattgat gagattgtgg gagagacagc aagtgaggca gacagcagtg
     9301 agacctcagt ctctgaaaag gagaatgggc atgagaagga cgacgatgtt attcgctgta
     9361 tctgtggcct ctacaaggat gaaggtctca tgatccagtg tgacaagtgc atggtatggc
     9421 agcactgtga ttgtatggga gtgaactcag atgtggagca ctacctttgt gagcagtgtg
     9481 acccaaggcc tgtggacagg gaggttccca tgatccctcg gccccactat gcccaacctg
     9541 gctgtgtcta cttcatctgt ttgctccgag atgacttgct gcttcgtcag ggtgactgtg
     9601 tgtatctgat gagggatagt cggcgcaccc ctgatggcca cccggtccgt cagtcctatc
     9661 gactgttatc tcacattaac cgagataaac ttgacatctt tcgcattgag aagctttgga
     9721 agaatgaaaa agaggaacgg tttgcctttg gtcaccatta tttccgtccc cacgaaacac
     9781 accactctcc atcccgtcgg ttctatcata atgaactatt tcgggtgcca ctctatgaga
     9841 tcattccctt ggaggctgta gtggggacct gctgtgtgtt ggacctttat acgtattgta
     9901 aagggagacc caaaggagta aaggagcaag atgtgtacat ctgtgattat cggcttgaca
     9961 agtcagcaca cctgttttac aagatccacc ggaaccgcta tcctgtctgc accaaaccct
    10021 atgcttttga tcacttcccc aagaagctca ctcccaaaaa agatttctcg cctcattacg
    10081 tcccagacaa ctacaagagg aatggaggac gatcatcctg gaagtctgag cgctcaaagc
    10141 cacccctaaa agacttgggc caggaggatg atgctctacc cttgattgaa gaggttctag
    10201 ccagtcaaga gcaagcagcc aatgagatac ccagcctgga ggagccagaa cgggaagggg
    10261 ccactgctaa cgtcagtgag ggtgaaaaaa aaacagagga aagtagtcaa gaaccccagt
    10321 caacctgtac ccctgaggaa cgacggcata accaacggga acgactcaac cagatcttgc
    10381 tcaatctcct tgaaaaaatc cctggaaaaa atgccattga tgtgacctac ttgctggagg
    10441 aaggatcagg caggaaactg cgaaggcgta ctttgtttat cccagaaaac agctttcgaa
    10501 agtagggcct catgggccca gctttcttgt acaaagtggt tgataacggg aagcctatcc
    10561 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt taaacggggg
    10621 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
    10681 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
    10741 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
    10801 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
    10861 cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc
    10921 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    10981 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    11041 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    11101 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    11161 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    11221 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    11281 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    11341 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    11401 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    11461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    11521 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    11581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
    11641 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    11701 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
    11761 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt
    11821 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
    11881 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
    11941 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
    12001 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
    12061 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
    12121 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
    12181 cctctggtta tgtgtgggag ggctaagcac ttcgtggccg aggagcagga ctgacacgtg
    12241 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    12301 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    12361 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    12421 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    12481 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
    12541 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    12601 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    12661 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    12721 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    12781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    12841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    12901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    12961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    13021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    13081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    13141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    13201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    13261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    13321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    13381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    13441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    13501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    13561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    13621 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    13681 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    13741 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    13801 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    13861 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    13921 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    13981 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    14041 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    14101 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    14161 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    14221 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    14281 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    14341 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    14401 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    14461 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    14521 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    14581 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    14641 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    14701 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    14761 taagccagta tctgctccct gcttgtgtgt tggaggt
//
