LOCUS       sequence-437373-       11175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expresses the rabbit Iron Regulatory Protein fused to the 
            Gal 4 Activation Domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Bernstein DS, Buter N, Stumpf C, Wickens M
  TITLE     Analyzing mRNA-protein complexes using a yeast three-hybrid system.
  JOURNAL   Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4.
  PUBMED    12054889
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Methods"; date: "2002-02"; volume: "26"; issue: "2"; pages: 
            "123-41"
COMMENT     Sequence Label: pAD-IRP
FEATURES             Location/Qualifiers
     source          1..11175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      239..1581
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    571..618
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2058..2463
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2464..3558
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     protein_bind    4067..4100
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4178..4211
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4255..4288
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4366..4399
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4443..4476
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4554..4587
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(4608..4795)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     4716..4740
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8093..8119)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(8099..8125)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(8138..8479)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             complement(8495..8515)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(8561..8962)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(8991..9013)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9099..9239
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9154..9173
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9254..9271)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9425..10013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9505..9524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10184..10975,10976..11044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10807..10826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11045..11149)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agctttgaag aaaaatgcgc cttattcaat ctttgctata aaaaatggcc caaaatctca
       61 cattggaaga catttgatga cctcatttct ttcaatgaag ggcctaacgg agttgactaa
      121 tgttgtggga aattggagcg ataagcgtgc ttctgccgtg gccaggacaa cgtatactca
      181 tcagataaca gcaatacctg atcactactt cgcactagtt tctcggtact atgcatatga
      241 tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca
      301 gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg
      361 gataatatca caggaggtac tagactacct ttcatcctac ataaatagac gcatataagt
      421 acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca
      481 acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt
      541 ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct
      601 agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa gacgcacttt
      661 caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga ataccgcttc
      721 cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat ccctatataa
      781 cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct acatttttta
      841 tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta ttcatagagt
      901 gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag agacaaaata
      961 gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc actttctgtt
     1021 cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct ttatcttgaa
     1081 aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt caggcttttt
     1141 ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg acctacagtg
     1201 caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa gtaatctaag
     1261 atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa aaagaagtat
     1321 agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta aaaatgcagc
     1381 tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt acaaaaatga
     1441 agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt ctgtaaaaat
     1501 gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttctacaaa
     1561 atgaagcaca gatgcttcgt taacaaagat atgctattga agtgcaagat ggaaacgcag
     1621 aaaatgaacc ggggatgcga cgtgcaagat tacctatgca atagatgcaa tagtttctcc
     1681 aggaaccgaa atacatacat tgtcttccgt aaagcgctag actatatatt attatacagg
     1741 ttcaaatata ctatctgttt cagggaaaac tcccaggttc ggatgttcaa aattcaatga
     1801 tgggtaacaa gtacgatcgt aaatctgtaa aacagtttgt cggatattag gctgtatctc
     1861 ctcaaagcgt attcgaatat cattgagaag ctgcagcgtc acatcggata ataatgatgg
     1921 cagccattgt agaagtgcct tttgcatttc tagtctcttt ctcggtctag ctagttttac
     1981 tacatcgcga agatagaatc ttagatcaca ctgcctttgc tgagctggat caatagagta
     2041 acaaaagagt ggtaaggaac tgtgggaata ctcaggtatc gtaagatgca agagttcgaa
     2101 tctcttagca accattattt ttttcctcaa cataacgaga acacacaggg gcgctatcgc
     2161 acagaatcaa attcgatgac tggaaatttt ttgttaattt cagaggtcgc ctgacgcata
     2221 tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg gaaccggctt
     2281 ttcatataga atagagaagc gttcatgact aaatgcttgc atcacaatac ttgaagttga
     2341 caatattatt taaggaccta ttgttttttc caataggtgg ttagcaatcg tcttactttc
     2401 taacttttct taccttttac atttcagcaa tatatatata tatttcaagg atataccatt
     2461 ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc
     2521 acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc
     2581 gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt tccacttcca
     2641 gatgaggcgc tggaagcctc caagaaggct gatgccgttt tgttaggtgc tgtgggtggt
     2701 cctaaatggg gtacaggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa
     2761 cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta
     2821 tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgtcag agaattagtg
     2881 ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt
     2941 gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta
     3001 caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt ggcctcttca
     3061 agattatgga gaaaaactgt ggaggaaacc atcaagaacg aatttcctac attgaaggtt
     3121 caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat
     3181 ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc
     3241 ccaggttcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc
     3301 gcatttggtt tgtacgaacc atgccacggt tctgctccag atttgccaaa gaataaggtc
     3361 aaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct
     3421 gaagaaggta aggccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact
     3481 ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt
     3541 aagaaaatcc ttgcttaaaa agattctctt tttttatgat atttgtacat aaactttata
     3601 aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga
     3661 cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga ggatgtaaag
     3721 gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa gaattgcact
     3781 ttaacattaa tattgacaag gaggagggca ccacacaaaa agttaggtgt aacagaaaat
     3841 catgaaacta tgattcctaa tttatatatt ggaggatttt ctctaaaaaa aaaaaaatac
     3901 aacaaataaa aaacactcaa tgacctgacc atttgatgga gtttaagtca ataccttctt
     3961 gaaccatttc ccataatggt gaaagttccc tcaagaattt tactctgtca gaaacggcct
     4021 taacgacgta gtcgaccaat tccgatcata ttcaataacc cttaatataa cttcgtataa
     4081 tgtatgctat acgaagttat taggtctgaa gaggagttta cgtccagcca agctagcttg
     4141 gctgcaggtc gagcggccgc gatccggaac ccttaatata acttcgtata atgtatgcta
     4201 tacgaagtta ttaggtccct cgaccaattc cgatcatatt caataaccct taatataact
     4261 tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag
     4321 ctagcttggc tgcaggtcga gcggccgcga tccggaaccc ttaatataac ttcgtataat
     4381 gtatgctata cgaagttatt aggtccctcg accaattccg atcatattca ataaccctta
     4441 atataacttc gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc
     4501 cagccaagct agcttggctg caggtcgagc ggccgcgatc cggaaccctt aatataactt
     4561 cgtataatgt atgctatacg aagttattag gtccctcgac cggcatgccg gtagaggtgt
     4621 ggtcaataag agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt
     4681 tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac
     4741 ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt
     4801 tagaagtgtc aacaacgtat ctaccaacga tttgaccctt ttccatcttt tcgtaaattt
     4861 ctggcaaggt agacaagccg acaaccttga ttggagactt gaccaaacct ctggcgaaga
     4921 agtccaaagc ttctgaataa gccctcgtaa tatattttca tgaagaattt aggtccaaaa
     4981 aaaagatggg cattaattct agtcatttaa aaaattctat agatcagagg ttacatggcc
     5041 aagattgaaa cttagaggag tatagttaca taaaagaagg caaaacgatg tataaatgaa
     5101 agaaattgag atggtgcacg atgcacagtt gaagtgaact tgcggggttt ttcagtatct
     5161 acgattcata gatctctcga gctcgaattc ggatccccct agaaaaagac agaggaaagc
     5221 agagtgtaga aacttctggt tttgtgcctc caggattggc accaaacccc agtgctcact
     5281 cgctgtctgt gaggcaggag cccccgtgga ggcagggaag agctcctgca gaagggtctc
     5341 tgctggctag cggctgggcc ttctccctgg ggcagcacca agtacaggtt tcctaagaac
     5401 cgagcgccta cttggccatc ttgcggatca tgtagttgag tatgccgcca ttgtggaaat
     5461 aagtgagctc cacgtcagtg tcaaaccgta tgacggcctg gaaggtcttc ccggtgtcca
     5521 gcttgacctg gacatgcatc cgtggtgtga ggttttctgg aatgatgatg gtgtatcgtt
     5581 cccgtcctgt gagtcccagg gagtctgcgt tctcaccagg gaggtattca agggggatca
     5641 cccccattcc aaccaggtta ctgcggtgaa tgcgctcgta actctcagcc aggacagctt
     5701 tgattcccag caggaaaggg cccttggctg cccagtctcg ggagctgcct gagccatact
     5761 ccttgccagc aaggacgatc aggggatggc cttcctgctg gtaccgctca gctgcatcaa
     5821 acacatcaag ggtttctcca gaaggcagat gtatagtctg tggtgcctgc ttgttcaaaa
     5881 atctgtttaa caagcgaatg ttggcgaacg tcccccgtgc catgatggca tcattccccc
     5941 ggcgggagcc ataggagttg aattctcgtg gagttagccc tctgttggtt aagtagcgag
     6001 cagcagggct atttcttgca atatttccag ctggagagat gtggtccgtc gttactgaat
     6061 ctcccaaatt taacagcaca taggcatcca ctatagattt agggggctgc aggtccaaag
     6121 tcaggttttc aaagaatggt ggtgacttaa tgtatgtgga tttgggattc cagagataca
     6181 gcttatctga tggggctgct aaggcattcc agcttgcatt cacagtctct attttctggt
     6241 agacctccgt gaacatccca gggatgacgt actgccgctc cactgcctgg atctcctctc
     6301 gagttggcca gatatctctc agaaacacct gctgcccctt tgcgttcgtc cccaatggct
     6361 ctttctcaaa gtcgatcctg atggtcccag caattgcata tgctattact aagggaggag
     6421 aggccaaata gttggcccgg gtgttgggat gaactcgacc ttcaaaattt ctgttcccag
     6481 agagtactcc gacagctaca aggtctccct gtgtgatggc ttctaccaca ggctcaggta
     6541 agggtccact gttgccgatg caggtcatgc agccatagcc caccacatca aaccccagct
     6601 gagacaagta aggcatgact ccactctctc gcaggtagta ggtgaccaca ccacttccag
     6661 gggacaggct ggtcttcacg tagggcttca cgttcaagcc agcatccacg gctttcttcg
     6721 ctaacagtcc tgctcctaac atcacagatg gattactggt gtttgtgcag ctagtaatgg
     6781 cagcaattac cacagatcca tgagaaaggg tgaattcact atcattatag ataaacgtct
     6841 tatggtcatt atgatggtct ggagcaacct ggaatccttt aaatccttgc ttggctccaa
     6901 ggcagctctc aaagtccttt ttcatatcag acacagcaac tttgtcctga ggcctcttgg
     6961 gtccactgca gcaaggcact acggtcttca aatctaattc cacaacctgg gtgaagtctg
     7021 ggtcttgaga ggggtcactg taatctcgga acatccctac agcctgaaga tactttctaa
     7081 tctgcttcac tttgctttca tcccgacctg tctgcaccaa gtacttgata ctgacttcat
     7141 caactgggaa gaaggtagca gttgctccgt attctggaca catgttggcg attgtagctc
     7201 tgtcagcaat ggacaactgg gctactcctg gcccgaagaa ctcaacaaat ttgcccacga
     7261 ccccaacctg gcggaggtgc ttggtaatgg tgagcacgat gtcagtggat gttaccagag
     7321 ggtggggctt tcccatcagc ctgtagccaa tcacctgggg aagcaccata ctgataggct
     7381 gacccagcat gacagcttct gcttcaatgc cacccacacc ccagccaaga acgcccaaac
     7441 cgtcaatcat ggtcgtgtgc gaatcggtgc ccacgaggct gtcggggtag tagtatccat
     7501 cctgatcaaa cactaccctg gccaagtatt ccagattcac ttggtggatg attcctgagc
     7561 cgggagggat aatccgcatg tttcgaaaag ccttggaacc ccactttaaa aattcaaatc
     7621 gttctctatt tctttcaaat tctaggtctt gattcttctg taagctgtca gcccttctgt
     7681 tgaaatcaac ctggatggag tgatcgatta caagatcaac aggacagata gggtttattt
     7741 tctctggatc accccctaat tttttcacag catcacgcat tgcagcaaag tcaaccacag
     7801 atggtacacc cgtgaagtcc tgcaggatga cacgggcggg tttgaatggc acttctatgt
     7861 tcatatgctg cgtgacattc cagttcagga tattttcaat gtcttctttc ttcaccaaaa
     7921 acttatcaca gttccgaacg gctgcctcca ggagaactct gattgaaaac ggtaagcgtc
     7981 catatcttga atagtctaat ttattcaaat tgaagaactt ctttcctggt tgtgcaggat
     8041 ctaacggctc agcaaggtat gcaaatgggt tgctcatggc catatgacca cccaagctag
     8101 cgtaatctgg aacatcgtat gggtaagcca tagagatctc tttttttggg tttggtgggg
     8161 tatcttcatc atcgaataga tagttatata catcatccat tgtagtggta ttaaacatcc
     8221 ctgtagtgat tccaaacgcg ttatacgcag tttggtccgt ccaaccaggt gacagtggtt
     8281 ttgaattatt accatcatca attttactag ccgtgatttc attattcatg aagttatcat
     8341 gaacgttaga ggaggcaatt ggttgtgaaa gcgcttgaga atttgtttga gttgttatga
     8401 ggttcggacc gttgctactg ttagtgaaag tgaaggacaa tgagctatca gcaatattcc
     8461 cactttgatt aaaattggcg gcggtaccca attcgacctt tctcttcttt tttggaggct
     8521 cgggaattaa ttccgcttta tccatctttg caaagcttgg agttgattgt atgcttggta
     8581 tagcttgaaa tattgtgcag aaaaagaaac aaggaagaaa gggaacgaga acaatgacga
     8641 ggaaacaaaa gattaataat tgcaggtcta tttatacttg atagcaagac agcaaacttt
     8701 tttttatttc aaattcaagt aactggaagg aaggccgtat accgttgctc attagagagt
     8761 agtgtgcgtg aatgaaggaa ggaaaaagtt tcgtgtgctt cgagataccc ctcatcagct
     8821 ctggaacaac gacatctgtt ggtgctgtct ttgtcgttaa ttttttcctt tagtgtcttc
     8881 catcattttt ttgtcattgc ggatatggtg agacaacaac gggggagaga gaaaagaaaa
     8941 aaaaagaaaa gaagttgctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     9001 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     9061 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     9121 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     9181 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9241 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9301 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9361 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9421 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9481 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9541 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9601 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9661 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9721 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9781 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9841 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     9901 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9961 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    10021 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    10081 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    10141 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10201 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10261 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10321 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10381 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10441 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
    10501 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10561 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10621 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10681 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10741 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10801 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10861 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10921 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10981 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    11041 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11101 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    11161 gaaaagtgcc acctg
//
