LOCUS       sequence-43742-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: DNMT3B (3QKJ)
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(512..532)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(545..562)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             572..594
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    609..633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(633..652)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(634..652)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(736..755)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttcgtcatc
       61 aaggctgggt gttattcggc ttcagcccct cgattccggt aggtttaaaa cccccgtgag
      121 cccactccag cataggcttc agctggtcct ccaggctgtc acccggacta gacgggaaag
      181 tcttacccgc acggacgcga gctttctcca gtgcgtggta catggcttta cgataagata
      241 ccagcttgtt aaacgtcgcc agattgaaat gctgcgagaa cagtcccaga gccaccagtt
      301 tgtctgcgga tacttctgag aatttcccgt caccaaacca ttggacccag cgcatgccac
      361 tcatggcctg acgcttagag gtagccttcc aagacaccac catggcgggc caccaggaga
      421 agcccttgat ctttccccac acgaggtccc ctattccaaa ctccttccca tcctgatact
      481 ctgaactgtc tccatctcca ctgtctgcct cgccctggaa atacaagttt tctctgccgc
      541 tgctgtgatg atgatgatga tgcatggtat atctccttct taaagttaaa caaaattatt
      601 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      661 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      721 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      781 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
      841 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc a
//
