LOCUS       sequence-43743-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MLH1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(86..133)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     129..147
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(200..217)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
       61 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
      121 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      181 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttcg
      241 tcatcatttg tatagatcag gcaggttagc aagctgcagg atatttccat cttctgtgaa
      301 atgtttagga ggcagaatgt gtgagcgcaa ggctttatag acaatgtgtt ccacagtcca
      361 cttccaggag tttggaatgg agccaggcac ttcactctgc tggcctgaga gggtcgactc
      421 ctcagatatg tactgcttcc ggatggaata gaacatagcg cattctttac tgaggctttc
      481 aaaacattcc ttttcttcgt cccaattcac ctcagtggct agtcgaagaa tgaagatagg
      541 cagtccctcc aaagggggca catagttgtc aatcagaagg ggtaatccaa tcaggttccc
      601 ttcctcatca atttccaaag agaaatagtc tgcaagcatc tcagccttct tcttcagaaa
      661 ctcaacaatg tattcagcaa gtccttcttt gggaccatct tcctctgtcc agccactctc
      721 tggactatct aaggcaagca tggcaaggtc aaagagcggt gctggctccg ataacctgag
      781 aacaccaaaa ttggcaaaat cataaatgag tatctggtag aacagttctt cactaa
//
