LOCUS       sequence-437436-       11074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaIDR7KTG in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11074)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdIDR7KTG
FEATURES             Location/Qualifiers
     source          1..11074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5533..5542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7021..7740
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    7862..7983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7899..7918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7953..7972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7990..8445)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8127..8148)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8339..8358
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8472..8576
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8578..8935
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8578..8769
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8601..8621)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8786..8921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8970..9764
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8970..9764
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9024..9043)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9634..9653
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9952..9971)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9996..10043
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10372..10960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10861..10880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgaaacc aacaaaccat cctggggtct ccagctagtg gaattcaaaa cacaattggt
     2041 tctgttggca cagggcaaca gaatgccact tctttaagta acccaaatcc catagacccc
     2101 agctccatgc agcgagccta tgctgctctc ggactcccct acatgaacca gccccagacg
     2161 cagctgcagc ctcaggttcc tggccagcaa ccagcacagc ctcaaaccca ccagcagatg
     2221 aggactctca accccctggg aaataatcca atgaacattc cagcaggagg aataacaaca
     2281 gatcagcagc ccccaaactt gatttcagaa tcagctcttc cgacttccct gggggccaca
     2341 aacccactga tgaacgatgg ctccaactct ggtaacattg gaaccctcag cactatacca
     2401 acagcagctc ctccttctag caccggtgta aggaaaggct ggcacgaaca tgtcactcag
     2461 gacctgcgga gccatctagt gcataaactc gtccaagcca tcttcccaac acctgatccc
     2521 gcagctctaa aggatcgccg catggaaaac ctggtagcct atgctaagaa agtggaaggg
     2581 gacatgtacg agtctgccaa cagcagggat gaatattatc acttattagc agagaaaatc
     2641 tacaagatac aaaaagaact agaagaaaaa cggaggtcgc gtttacataa acaaggcatc
     2701 ttggggaacc agccagcctt accagccccg ggggctcagc cccctgtgat tccacaggca
     2761 caacctgtga gacctccaaa tggacccctg tccctgccag tgaatcgcat gcaagtttct
     2821 caagggatga attcatttaa ccccatgtcc ttggggaacg tccagttgcc acaagcaccc
     2881 atgggacctc gtgcagcctc cccaatgaac cactctgtcc agatgaacag catgggctca
     2941 gtgccaggga tggccatttc tccttcccga atgcctcagc ctccgaacat gatgggtgca
     3001 cacaccaaca acatgatggc ccaggcgccc gctcagagcc agtttctgcc acagaaccag
     3061 ttcccgtcat ccagcggggc gatgagtgtg ggcatggggc agccgccagc ccaaacaggc
     3121 gtgtcacagg gacaggtgcc tggtgctgct cttcctaacc ctctcaacat gctggggcct
     3181 caggccagcc agctaccttg ccctccagtg acacagtcac cactgcaccc aacaccgcct
     3241 cctgcttcca cggctgctgg catgccatct ctccagcaca cgacaccacc tgggatgact
     3301 cctccccagc cagcagctcc cactcagcca tcaactcctg tgtcgtcttc cgggcagact
     3361 cccaccccga ctcctggctc agtgcccagt gctacccaaa cccagagcac ccctacagtc
     3421 caggcagcag cccaggccca ggtgaccccg cagcctcaaa ccccagttca gcccccgtct
     3481 gtggctaccc ctcagtcatc gcagcaacag ccgacgcctg tgcacgccca gcctcctggc
     3541 acaccgcttt cccaggcagc agccagcatt gataacagag tccctacccc ctcctcggtg
     3601 gccagcgcag aaaccaattc ccagcagcca ggacctgacg tacctgtgct ggaaatgaag
     3661 acggagaccc aagcagagga cactgagccc gatcctggtg aatccaaagg ggagcccagg
     3721 tctgagatga tggaggagga tttgcaagga gcttcccaag ttaaagaaga aacagacata
     3781 gcagagcaga aatcagaacc aatggaagtg gatgaaaaga aacctgaagt gaaagtagaa
     3841 gttaaagagg aagaagagag tagcagtaac ggcacagcct ctcagtcaac atctccttcg
     3901 cagccgcgca aaaaaatctt taaaccagag gagttacgcc aggccctcat gccaacccta
     3961 gaagcactgt atcgacagga cccagagtca ttacctttcc ggcagcctgt agatccccag
     4021 ctcctcggaa ttccagacta ttttgacatc gtaaagaatc ccatggacct ctccaccatc
     4081 aagcggaagc tggacacagg gcaataccaa gagccctggc agtacgtgga cgacgtctgg
     4141 ctcatgttca acaatgcctg gctctataat cgcaagacat cccgagtcta taagttttgc
     4201 agtaagcttg cagaggtctt tgagcaggaa attgaccctg tcatgcagtc ccttggatat
     4261 tgctgtggac gcaagtatga gttttcccca cagactttgt gctgctatgg gaagcagctg
     4321 tgtaccattc ctcgcgatgc tgcctactac agctatcaga ataggtatca tttctgtgag
     4381 aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc ttcacagccc
     4441 cagacgacaa tttcaaagga tcagtttgaa aagaagaaaa atgatacctt agaccccgaa
     4501 cctttcgttg attgcaagga gtgtggccgg aagatgcatc agatttgcgt tctgcactat
     4561 gacatcattt ggccttcagg ttttgtgtgc gacaactgct tgaagaaaac tggcagacct
     4621 cgaaaagaaa acaaattcag tgctaagagg ctgcagacca caagactggg aaaccacttg
     4681 gaagaccgag tgaacaaatt tttgcggcgc cagaatcacc ctgaagccgg ggaggttttt
     4741 gtccgagtgg tggccagctc agacaagacg gtggaggtca agcccgggat gaagtcacgg
     4801 tttgtggatt ctggggaaat gtctgaatct ttcccatatc gaaccaaagc tctgtttgct
     4861 tttgaggaaa ttgacggcgt ggatgtctgc ttttttggaa tgcacgtcca agaatacggc
     4921 tctgattgcc cccctccaaa cacgaggcgt gtgtacattt cttatctgga tagtattcat
     4981 ttcttccggc cacgttgcct ccgcacagcc gtttaccatg agatccttat tggatattta
     5041 gagtatgtga agaaattagg gtatgtgaca gggcacatct gggcctgtcc tccaagtgaa
     5101 ggagatgatt acatcttcca ttgccaccca cctgatcaaa aaatacccaa gccaaaacga
     5161 ctgcaggagt ggtacaaaaa gatgctggac aaggcgtttg cagagcggat catccatgac
     5221 tacaaggata ttttcaaaca agcaactgaa gacaggctca ccagtgccaa ggaactgccc
     5281 tattttgaag gtgatttctg gcccaatgtg ttagaagaga gcattaagga actagaacaa
     5341 gaagaagagg agaggggtgg cgaagagagc actgcagcca gtgaaaccac tgagggcagt
     5401 cagggcgaca gcgggaatgc cggcggtggg aacaacggcg gtaccaacgg gaacggtagc
     5461 agcatcagcc gcgccaacgg cggtgggccc agcatgccca acgtgtccaa tgacctgtcc
     5521 cagaagctgt atgccaccat ggagaagcac aaggaggtct tcttcgtgat ccacctgcac
     5581 gctgggcctg tcatcaacac cctgcccccc atcgtcgacc ccgaccccct gctcagctgt
     5641 gacctcatgg atgggcgcga cgccttcctc accctcgcca gagacaagca ctgggagttc
     5701 tcctccttgc gccgctccaa gtggtccacg ctctgcatgc tggtggagct gcacacccag
     5761 ggccaggacc gctttgtcta cacctgcaac gagtgcaagc accacgtgga gacgcgctgg
     5821 cactgcactg tgtgcgagga ctacgacctc tgcatcaact gctataacac gaagagccat
     5881 gcccataaga tggtgaagtg ggggctgggc ctggatgacg agggcagcag ccagggcgag
     5941 ccacagtcaa agagccccca ggagtcacgc cggctgagca tccagcgctg catccagtcg
     6001 ctggtgcacg cgtgccagtg ccgcaacgcc aactgctcgc tgccatcctg ccagaagatg
     6061 aagcgggtgg tgcagcacac caagggctgc aaacgcaaga ccaacggggg ctgcccggtg
     6121 tgcaagcagc tcatcgccct ctgctgctac cacgccaagc actgccaaga aaacaaatgc
     6181 cccgtgccct tctgcctcaa catcaaacac aagctccgcc agcagcagat ccagcaccgc
     6241 ctgcagcagg cccagctcat gcgccggcgg atggccacca tgaacacccg caacgtgcct
     6301 cagcagagtc tgccttctcc tacctcagca ccgcccggga cccccacaca gcagcccagc
     6361 acaccccaga cgccgcagcc ccctgcccag ccccaaccct cacccgtgag catgtcacca
     6421 gctggcttcc ccagcgtggc ccggactcag ccccccacca cggtgtccac agggaagcct
     6481 accagccagg tgccggcccc cccacccccg gcccagcccc ctcctgcagc ggtggaagcg
     6541 gctcggcaga tcgagcgtga ggcccagcag cagcagcacc tgtaccgggt gaacatcaac
     6601 aacagcatgc ccccaggacg cacgggcatg gggaccccgg ggagccagat ggcccccgtg
     6661 agcctgaatg tgccccgacc caaccaggtg agcgggcccg tcatgcccag catgcctccc
     6721 gggcagtggc agcaggcgcc ccttccccag cagcagccca tgccaggctt gcccaggcct
     6781 gtgatatcca tgcaggccca ggcggccgtg gctgggcccc ggatgcccag cgtgcagcca
     6841 cccaggagca tctcacccag cgctctgcaa gacctgctgc ggaccctgaa gtcgcccagc
     6901 tcccctcagc agcaacagca ggtgctgaac attctcaaat caaacccgca gctaatggca
     6961 gctttcatca aacagcgcac agccaagtac ctagagaagt ttgtggaggg cttgccggtc
     7021 atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat
     7081 ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga tgccacctac
     7141 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ctggcccacc
     7201 ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga tcacatgaag
     7261 cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg caccatcttc
     7321 ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg cgataccctg
     7381 gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat cctgggcaat
     7441 aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa ggccaagaat
     7501 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt gcagctggcc
     7561 gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc cgataaccac
     7621 tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga tcacatgatc
     7681 tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct gtacaagtga
     7741 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7801 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7861 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7981 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     8041 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     8101 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     8161 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     8221 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     8281 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     8341 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     8401 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     8461 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8521 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     8581 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8641 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8701 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8761 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8821 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8881 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     8941 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     9001 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     9061 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     9121 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     9181 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     9241 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     9301 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     9361 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     9421 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     9481 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     9541 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     9601 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     9661 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     9721 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     9781 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     9841 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9901 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     9961 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
    10021 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
    10081 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
    10141 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
    10201 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
    10261 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10321 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10381 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10441 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10501 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10561 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10621 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10681 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10741 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10801 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10861 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10921 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10981 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    11041 tgattctgtg gataaccgta ttaccgccat gcat
//
