LOCUS       sequence-437439-       11611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaIDR3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11611)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdIDR3
FEATURES             Location/Qualifiers
     source          1..11611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5092..5101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7558..8277
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    8399..8520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8436..8455)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8490..8509
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8527..8982)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8664..8685)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8876..8895
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9009..9113
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9115..9472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9115..9306
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9138..9158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9323..9458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9507..10301
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9507..10301
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9561..9580)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10171..10190
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10489..10508)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10533..10580
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10909..11497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11398..11417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgagtaa ggaaaggctg gcacgaacat gtcactcagg acctgcggag ccatctagtg
     2041 cataaactcg tccaagccat cttcccaaca cctgatcccg cagctctaaa ggatcgccgc
     2101 atggaaaacc tggtagccta tgctaagaaa gtggaagggg acatgtacga gtctgccaac
     2161 agcagggatg aatattatca cttattagca gagaaaatct acaagataca aaaagaacta
     2221 gaagaaaaac ggaggtcgcg tttacataaa caaggcatct tggggaacca gccagcctta
     2281 ccagccccgg gggctcagcc ccctgtgatt ccacaggcac aacctgtgag acctccaaat
     2341 ggacccctgt ccctgccagt gaatcgcatg caagtttctc aagggatgaa ttcatttaac
     2401 cccatgtcct tggggaacgt ccagttgcca caagcaccca tgggacctcg tgcagcctcc
     2461 ccaatgaacc actctgtcca gatgaacagc atgggctcag tgccagggat ggccatttct
     2521 ccttcccgaa tgcctcagcc tccgaacatg atgggtgcac acaccaacaa catgatggcc
     2581 caggcgcccg ctcagagcca gtttctgcca cagaaccagt tcccgtcatc cagcggggcg
     2641 atgagtgtgg gcatggggca gccgccagcc caaacaggcg tgtcacaggg acaggtgcct
     2701 ggtgctgctc ttcctaaccc tctcaacatg ctggggcctc aggccagcca gctaccttgc
     2761 cctccagtga cacagtcacc actgcaccca acaccgcctc ctgcttccac ggctgctggc
     2821 atgccatctc tccagcacac gacaccacct gggatgactc ctccccagcc agcagctccc
     2881 actcagccat caactcctgt gtcgtcttcc gggcagactc ccaccccgac tcctggctca
     2941 gtgcccagtg ctacccaaac ccagagcacc cctacagtcc aggcagcagc ccaggcccag
     3001 gtgaccccgc agcctcaaac cccagttcag cccccgtctg tggctacccc tcagtcatcg
     3061 cagcaacagc cgacgcctgt gcacgcccag cctcctggca caccgctttc ccaggcagca
     3121 gccagcattg ataacagagt ccctaccccc tcctcggtgg ccagcgcaga aaccaattcc
     3181 cagcagccag gacctgacgt acctgtgctg gaaatgaaga cggagaccca agcagaggac
     3241 actgagcccg atcctggtga atccaaaggg gagcccaggt ctgagatgat ggaggaggat
     3301 ttgcaaggag cttcccaagt taaagaagaa acagacatag cagagcagaa atcagaacca
     3361 atggaagtgg atgaaaagaa acctgaagtg aaagtagaag ttaaagagga agaagagagt
     3421 agcagtaacg gcacagcctc tcagtcaaca tctccttcgc agccgcgcaa aaaaatcttt
     3481 aaaccagagg agttacgcca ggccctcatg ccaaccctag aagcactgta tcgacaggac
     3541 ccagagtcat tacctttccg gcagcctgta gatccccagc tcctcggaat tccagactat
     3601 tttgacatcg taaagaatcc catggacctc tccaccatca agcggaagct ggacacaggg
     3661 caataccaag agccctggca gtacgtggac gacgtctggc tcatgttcaa caatgcctgg
     3721 ctctataatc gcaagacatc ccgagtctat aagttttgca gtaagcttgc agaggtcttt
     3781 gagcaggaaa ttgaccctgt catgcagtcc cttggatatt gctgtggacg caagtatgag
     3841 ttttccccac agactttgtg ctgctatggg aagcagctgt gtaccattcc tcgcgatgct
     3901 gcctactaca gctatcagaa taggtatcat ttctgtgaga agtgtttcac agagatccag
     3961 ggcgagaatg tgaccctggg tgacgaccct tcacagcccc agacgacaat ttcaaaggat
     4021 cagtttgaaa agaagaaaaa tgatacctta gaccccgaac ctttcgttga ttgcaaggag
     4081 tgtggccgga agatgcatca gatttgcgtt ctgcactatg acatcatttg gccttcaggt
     4141 tttgtgtgcg acaactgctt gaagaaaact ggcagacctc gaaaagaaaa caaattcagt
     4201 gctaagaggc tgcagaccac aagactggga aaccacttgg aagaccgagt gaacaaattt
     4261 ttgcggcgcc agaatcaccc tgaagccggg gaggtttttg tccgagtggt ggccagctca
     4321 gacaagacgg tggaggtcaa gcccgggatg aagtcacggt ttgtggattc tggggaaatg
     4381 tctgaatctt tcccatatcg aaccaaagct ctgtttgctt ttgaggaaat tgacggcgtg
     4441 gatgtctgct tttttggaat gcacgtccaa gaatacggct ctgattgccc ccctccaaac
     4501 acgaggcgtg tgtacatttc ttatctggat agtattcatt tcttccggcc acgttgcctc
     4561 cgcacagccg tttaccatga gatccttatt ggatatttag agtatgtgaa gaaattaggg
     4621 tatgtgacag ggcacatctg ggcctgtcct ccaagtgaag gagatgatta catcttccat
     4681 tgccacccac ctgatcaaaa aatacccaag ccaaaacgac tgcaggagtg gtacaaaaag
     4741 atgctggaca aggcgtttgc agagcggatc atccatgact acaaggatat tttcaaacaa
     4801 gcaactgaag acaggctcac cagtgccaag gaactgccct attttgaagg tgatttctgg
     4861 cccaatgtgt tagaagagag cattaaggaa ctagaacaag aagaagagga gaggaaaaag
     4921 gaagagagca ctgcagccag tgaaaccact gagggcagtc agggcgacag caagaatgcc
     4981 aagaagaaga acaacaagaa aaccaacaag aacaaaagca gcatcagccg cgccaacaag
     5041 aagaagccca gcatgcccaa cgtgtccaat gacctgtccc agaagctgta tgccaccatg
     5101 gagaagcaca aggaggtctt cttcgtgatc cacctgcacg ctgggcctgt catcaacacc
     5161 ctgcccccca tcgtcgaccc cgaccccctg ctcagctgtg acctcatgga tgggcgcgac
     5221 gccttcctca ccctcgccag agacaagcac tgggagttct cctccttgcg ccgctccaag
     5281 tggtccacgc tctgcatgct ggtggagctg cacacccagg gccaggaccg ctttgtctac
     5341 acctgcaacg agtgcaagca ccacgtggag acgcgctggc actgcactgt gtgcgaggac
     5401 tacgacctct gcatcaactg ctataacacg aagagccatg cccataagat ggtgaagtgg
     5461 gggctgggcc tggatgacga gggcagcagc cagggcgagc cacagtcaaa gagcccccag
     5521 gagtcacgcc ggctgagcat ccagcgctgc atccagtcgc tggtgcacgc gtgccagtgc
     5581 cgcaacgcca actgctcgct gccatcctgc cagaagatga agcgggtggt gcagcacacc
     5641 aagggctgca aacgcaagac caacgggggc tgcccggtgt gcaagcagct catcgccctc
     5701 tgctgctacc acgccaagca ctgccaagaa aacaaatgcc ccgtgccctt ctgcctcaac
     5761 atcaaacaca agctccgcca gcagcagatc cagcaccgcc tgcagcaggc ccagctcatg
     5821 cgccggcgga tggccaccat gaacacccgc aacgtgcctc agcagagtct gccttctcct
     5881 acctcagcac cgcccgggac ccccacacag cagcccagca caccccagac gccgcagccc
     5941 cctgcccagc cccaaccctc acccgtgagc atgtcaccag ctggcttccc cagcgtggcc
     6001 cggactcagc cccccaccac ggtgtccaca gggaagccta ccagccaggt gccggccccc
     6061 ccacccccgg cccagccccc tcctgcagcg gtggaagcgg ctcggcagat cgagcgtgag
     6121 gcccagcagc agcagcacct gtaccgggtg aacatcaaca acagcatgcc cccaggacgc
     6181 acgggcatgg ggaccccggg gagccagatg gcccccgtga gcctgaatgt gccccgaccc
     6241 aaccaggtga gcgggcccgt catgcccagc atgcctcccg ggcagtggca gcaggcgccc
     6301 cttccccagc agcagcccat gccaggcttg cccaggcctg tgatatccat gcaggcccag
     6361 gcggccgtgg ctgggccccg gatgcccagc gtgcagccac ccaggagcat ctcacccagc
     6421 gctctgcaag acctgctgcg gaccctgaag tcgcccagct cccctcagca gcaacagcag
     6481 gtgctgaaca ttctcaaatc aaacccgcag ctaatggcag ctttcatcaa acagcgcaca
     6541 gccaagtacg tggccaatca gcccggcatg cagccccagc ctggcctcca gtcccagccc
     6601 ggcatgcaac cccagcctgg catgcaccag cagcccagcc tgcagaacct gaatgccatg
     6661 caggctggcg tgccgcggcc cggtgtgcct ccacagcagc aggcgatggg aggcctgaac
     6721 ccccagggcc aggccttgaa catcatgaac ccaggacaca accccaacat ggcgagtatg
     6781 aatccacagt accgagaaat gttacggagg cagctgctgc agcagcagca gcaacagcag
     6841 cagcaacaac agcagcaaca gcagcagcag caagggagtg ccggcatggc tgggggcatg
     6901 gcggggcacg gccagttcca gcagcctcaa ggacccggag gctacccacc ggccatgcag
     6961 cagcagcagc gcatgcagca gcatctcccc ctccagggca gctccatggg ccagatggcg
     7021 gctcagatgg gacagcttgg ccagatgggg cagccggggc tgggggcaga cagcaccccc
     7081 aacatccagc aagccctgca gcagcggatt ctgcagcaac agcagatgaa gcagcagatt
     7141 gggtccccag gccagccgaa ccccatgagc ccccagcaac acatgctctc aggacagcca
     7201 caggcctcgc atctccctgg ccagcagatc gccacgtccc ttagtaacca ggtgcggtct
     7261 ccagcccctg tccagtctcc acggccccag tcccagcctc cacattccag cccgtcacca
     7321 cggatacagc cccagccttc gccacaccac gtctcacccc agactggttc cccccacccc
     7381 ggactcgcag tcaccatggc cagctccata gatcagggac acttggggaa ccccgaacag
     7441 agtgcaatgc tcccccagct gaacaccccc agcaggagtg cgctgtccag cgaactgtcc
     7501 ctggtcgggg acaccacggg ggacacgcta gagaagtttg tggagggctt gccggtcatg
     7561 gtgagcaagg gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc
     7621 gatgtgaatg gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc
     7681 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg
     7741 gtgaccaccc tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag
     7801 cacgacttct tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc
     7861 gaggatgacg gcaactacaa gtcgcgcgcc gaggtgaagt tcgagggcga taccctggtg
     7921 aatcgcatcg agctgaccgg caccgatttc aaggaggatg gcaacatcct gggcaataag
     7981 atggagtaca actacaacgc ccacaatgtg tacatcatga ccgacaaggc caagaatggc
     8041 atcaaggtga acttcaagat ccgccacaac atcgaggatg gcagcgtgca gctggccgac
     8101 cactaccagc agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac
     8161 ctgtccaccc agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac
     8221 ttcggcttcg tgaccgccgc cgccatcacc cacggcatgg atgagctgta caagtgagcg
     8281 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     8341 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     8401 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8461 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8521 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     8581 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     8641 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8701 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8761 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8821 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8881 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8941 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     9001 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9061 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     9121 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     9481 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     9541 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     9601 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     9661 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     9721 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     9781 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     9841 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     9901 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     9961 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    10021 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    10081 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    10141 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    10201 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    10261 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
    10321 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
    10381 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    10441 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
    10501 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
    10561 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
    10621 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
    10681 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
    10741 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10801 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    10921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    10981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    11401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    11521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    11581 ttctgtggat aaccgtatta ccgccatgca t
//
