LOCUS       sequence-437450-       15066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of CBP-GFP in mammalian cells by retroviral
            transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15066)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 15066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRetroX-TRE3G_CBP-GFP
FEATURES             Location/Qualifiers
     source          1..15066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             511..686
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    749..948
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1149..1565
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1495..1517
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1533..1549
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1605..1954
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1613..1631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1649..1667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1685..1703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1721..1739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1757..1775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1793..1811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1829..1847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1893..1917
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6828..6837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9294..10013
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        10044..10543
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10079..10102)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10476..10495
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10564..11589
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10564..11589
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             11770..12195
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(12298..12320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12456..12476)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12461..12790
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12641..12776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12812..12831
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12912..12929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13083..13671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13163..13182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13842..14633,14634..14702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14465..14484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14703..14807)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14875..14893
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(14993..15066,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgccccacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct caataaaaga gcccacaacc cctcactcgg
      541 cgcgccagtc ttccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt
      601 gctgtttgca tccgaatcgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
      661 actacccacg acgggggtct ttcatttggg ggctcgtccg ggatttggag acccctgccc
      721 agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg
      781 attgtctagt gtctatgttt gatgttatgc gcctgcgtct gtactagtta gctaactagc
      841 tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg
      901 ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga
      961 tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
     1021 tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc
     1081 tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
     1141 aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg
     1201 tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct
     1261 gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag
     1321 acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc
     1381 aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc
     1441 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
     1501 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
     1561 gcgccggaat tgaagatctc gatgaggccc tttcgtcttc actcgagttt actccctatc
     1621 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     1681 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     1741 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     1801 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg tatgtcgagg
     1861 taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc
     1921 tggagcaatt ccacaacact tttgtcttat acttggatcc aatggctgag aacttgctgg
     1981 acggaccgcc caaccccaaa agagccaaac tcagctcgcc cggtttctcg gcgaatgaca
     2041 gcacagattt tggatcattg tttgacttgg aaaatgatct tcctgatgag ctgataccca
     2101 atggaggaga attaggcctt ttaaacagtg ggaaccttgt tccagatgct gcttccaaac
     2161 ataaacaact gtcggagctt ctacgaggag gcagcggctc tagtatcaac ccaggaatag
     2221 gaaatgtgag cgccagcagc cccgtgcagc agggcctggg tggccaggct caagggcagc
     2281 cgaacagtgc taacatggcc agcctcagtg ccatgggcaa gagccctctg agccagggag
     2341 attcttcagc ccccagcctg cctaaacagg cagccagcac ctctgggccc acccccgctg
     2401 cctcccaagc actgaatccg caagcacaaa agcaagtggg gctggcgact agcagccctg
     2461 ccacgtcaca gactggacct ggtatctgca tgaatgctaa ctttaaccag acccacccag
     2521 gcctcctcaa tagtaactct ggccatagct taattaatca ggcttcacaa gggcaggcgc
     2581 aagtcatgaa tggatctctt ggggctgctg gcagaggaag gggagctgga atgccgtacc
     2641 ctactccagc catgcagggc gcctcgagca gcgtgctggc tgagacccta acgcaggttt
     2701 ccccgcaaat gactggtcac gcgggactga acaccgcaca ggcaggaggc atggccaaga
     2761 tgggaataac tgggaacaca agtccatttg gacagccctt tagtcaagct ggagggcagc
     2821 caatgggagc cactggagtg aacccccagt tagccagcaa acagagcatg gtcaacagtt
     2881 tgcccacctt ccctacagat atcaagaata cttcagtcac caacgtgcca aatatgtctc
     2941 agatgcaaac atcagtggga attgtaccca cacaagcaat tgcaacaggc cccactgcag
     3001 atcctgaaaa acgcaaactg atacagcagc agctggttct actgcttcat gctcataagt
     3061 gtcagagacg agagcaagca aacggagagg ttcgggcctg ctcgctcccg cattgtcgaa
     3121 ccatgaaaaa cgttttgaat cacatgacgc attgtcaggc tgggaaagcc tgccaagttg
     3181 cccattgtgc atcttcacga caaatcatct ctcattggaa gaactgcaca cgacatgact
     3241 gtcctgtttg cctccctttg aaaaatgcca gtgacaagcg aaaccaacaa accatcctgg
     3301 ggtctccagc tagtggaatt caaaacacaa ttggttctgt tggcacaggg caacagaatg
     3361 ccacttcttt aagtaaccca aatcccatag accccagctc catgcagcga gcctatgctg
     3421 ctctcggact cccctacatg aaccagcccc agacgcagct gcagcctcag gttcctggcc
     3481 agcaaccagc acagcctcaa acccaccagc agatgaggac tctcaacccc ctgggaaata
     3541 atccaatgaa cattccagca ggaggaataa caacagatca gcagccccca aacttgattt
     3601 cagaatcagc tcttccgact tccctggggg ccacaaaccc actgatgaac gatggctcca
     3661 actctggtaa cattggaacc ctcagcacta taccaacagc agctcctcct tctagcaccg
     3721 gtgtaaggaa aggctggcac gaacatgtca ctcaggacct gcggagccat ctagtgcata
     3781 aactcgtcca agccatcttc ccaacacctg atcccgcagc tctaaaggat cgccgcatgg
     3841 aaaacctggt agcctatgct aagaaagtgg aaggggacat gtacgagtct gccaacagca
     3901 gggatgaata ttatcactta ttagcagaga aaatctacaa gatacaaaaa gaactagaag
     3961 aaaaacggag gtcgcgttta cataaacaag gcatcttggg gaaccagcca gccttaccag
     4021 ccccgggggc tcagccccct gtgattccac aggcacaacc tgtgagacct ccaaatggac
     4081 ccctgtccct gccagtgaat cgcatgcaag tttctcaagg gatgaattca tttaacccca
     4141 tgtccttggg gaacgtccag ttgccacaag cacccatggg acctcgtgca gcctccccaa
     4201 tgaaccactc tgtccagatg aacagcatgg gctcagtgcc agggatggcc atttctcctt
     4261 cccgaatgcc tcagcctccg aacatgatgg gtgcacacac caacaacatg atggcccagg
     4321 cgcccgctca gagccagttt ctgccacaga accagttccc gtcatccagc ggggcgatga
     4381 gtgtgggcat ggggcagccg ccagcccaaa caggcgtgtc acagggacag gtgcctggtg
     4441 ctgctcttcc taaccctctc aacatgctgg ggcctcaggc cagccagcta ccttgccctc
     4501 cagtgacaca gtcaccactg cacccaacac cgcctcctgc ttccacggct gctggcatgc
     4561 catctctcca gcacacgaca ccacctggga tgactcctcc ccagccagca gctcccactc
     4621 agccatcaac tcctgtgtcg tcttccgggc agactcccac cccgactcct ggctcagtgc
     4681 ccagtgctac ccaaacccag agcaccccta cagtccaggc agcagcccag gcccaggtga
     4741 ccccgcagcc tcaaacccca gttcagcccc cgtctgtggc tacccctcag tcatcgcagc
     4801 aacagccgac gcctgtgcac gcccagcctc ctggcacacc gctttcccag gcagcagcca
     4861 gcattgataa cagagtccct accccctcct cggtggccag cgcagaaacc aattcccagc
     4921 agccaggacc tgacgtacct gtgctggaaa tgaagacgga gacccaagca gaggacactg
     4981 agcccgatcc tggtgaatcc aaaggggagc ccaggtctga gatgatggag gaggatttgc
     5041 aaggagcttc ccaagttaaa gaagaaacag acatagcaga gcagaaatca gaaccaatgg
     5101 aagtggatga aaagaaacct gaagtgaaag tagaagttaa agaggaagaa gagagtagca
     5161 gtaacggcac agcctctcag tcaacatctc cttcgcagcc gcgcaaaaaa atctttaaac
     5221 cagaggagtt acgccaggcc ctcatgccaa ccctagaagc actgtatcga caggacccag
     5281 agtcattacc tttccggcag cctgtagatc cccagctcct cggaattcca gactattttg
     5341 acatcgtaaa gaatcccatg gacctctcca ccatcaagcg gaagctggac acagggcaat
     5401 accaagagcc ctggcagtac gtggacgacg tctggctcat gttcaacaat gcctggctct
     5461 ataatcgcaa gacatcccga gtctataagt tttgcagtaa gcttgcagag gtctttgagc
     5521 aggaaattga ccctgtcatg cagtcccttg gatattgctg tggacgcaag tatgagtttt
     5581 ccccacagac tttgtgctgc tatgggaagc agctgtgtac cattcctcgc gatgctgcct
     5641 actacagcta tcagaatagg tatcatttct gtgagaagtg tttcacagag atccagggcg
     5701 agaatgtgac cctgggtgac gacccttcac agccccagac gacaatttca aaggatcagt
     5761 ttgaaaagaa gaaaaatgat accttagacc ccgaaccttt cgttgattgc aaggagtgtg
     5821 gccggaagat gcatcagatt tgcgttctgc actatgacat catttggcct tcaggttttg
     5881 tgtgcgacaa ctgcttgaag aaaactggca gacctcgaaa agaaaacaaa ttcagtgcta
     5941 agaggctgca gaccacaaga ctgggaaacc acttggaaga ccgagtgaac aaatttttgc
     6001 ggcgccagaa tcaccctgaa gccggggagg tttttgtccg agtggtggcc agctcagaca
     6061 agacggtgga ggtcaagccc gggatgaagt cacggtttgt ggattctggg gaaatgtctg
     6121 aatctttccc atatcgaacc aaagctctgt ttgcttttga ggaaattgac ggcgtggatg
     6181 tctgcttttt tggaatgcac gtccaagaat acggctctga ttgcccccct ccaaacacga
     6241 ggcgtgtgta catttcttat ctggatagta ttcatttctt ccggccacgt tgcctccgca
     6301 cagccgttta ccatgagatc cttattggat atttagagta tgtgaagaaa ttagggtatg
     6361 tgacagggca catctgggcc tgtcctccaa gtgaaggaga tgattacatc ttccattgcc
     6421 acccacctga tcaaaaaata cccaagccaa aacgactgca ggagtggtac aaaaagatgc
     6481 tggacaaggc gtttgcagag cggatcatcc atgactacaa ggatattttc aaacaagcaa
     6541 ctgaagacag gctcaccagt gccaaggaac tgccctattt tgaaggtgat ttctggccca
     6601 atgtgttaga agagagcatt aaggaactag aacaagaaga agaggagagg aaaaaggaag
     6661 agagcactgc agccagtgaa accactgagg gcagtcaggg cgacagcaag aatgccaaga
     6721 agaagaacaa caagaaaacc aacaagaaca aaagcagcat cagccgcgcc aacaagaaga
     6781 agcccagcat gcccaacgtg tccaatgacc tgtcccagaa gctgtatgcc accatggaga
     6841 agcacaagga ggtcttcttc gtgatccacc tgcacgctgg gcctgtcatc aacaccctgc
     6901 cccccatcgt cgaccccgac cccctgctca gctgtgacct catggatggg cgcgacgcct
     6961 tcctcaccct cgccagagac aagcactggg agttctcctc cttgcgccgc tccaagtggt
     7021 ccacgctctg catgctggtg gagctgcaca cccagggcca ggaccgcttt gtctacacct
     7081 gcaacgagtg caagcaccac gtggagacgc gctggcactg cactgtgtgc gaggactacg
     7141 acctctgcat caactgctat aacacgaaga gccatgccca taagatggtg aagtgggggc
     7201 tgggcctgga tgacgagggc agcagccagg gcgagccaca gtcaaagagc ccccaggagt
     7261 cacgccggct gagcatccag cgctgcatcc agtcgctggt gcacgcgtgc cagtgccgca
     7321 acgccaactg ctcgctgcca tcctgccaga agatgaagcg ggtggtgcag cacaccaagg
     7381 gctgcaaacg caagaccaac gggggctgcc cggtgtgcaa gcagctcatc gccctctgct
     7441 gctaccacgc caagcactgc caagaaaaca aatgccccgt gcccttctgc ctcaacatca
     7501 aacacaagct ccgccagcag cagatccagc accgcctgca gcaggcccag ctcatgcgcc
     7561 ggcggatggc caccatgaac acccgcaacg tgcctcagca gagtctgcct tctcctacct
     7621 cagcaccgcc cgggaccccc acacagcagc ccagcacacc ccagacgccg cagccccctg
     7681 cccagcccca accctcaccc gtgagcatgt caccagctgg cttccccagc gtggcccgga
     7741 ctcagccccc caccacggtg tccacaggga agcctaccag ccaggtgccg gcccccccac
     7801 ccccggccca gccccctcct gcagcggtgg aagcggctcg gcagatcgag cgtgaggccc
     7861 agcagcagca gcacctgtac cgggtgaaca tcaacaacag catgccccca ggacgcacgg
     7921 gcatggggac cccggggagc cagatggccc ccgtgagcct gaatgtgccc cgacccaacc
     7981 aggtgagcgg gcccgtcatg cccagcatgc ctcccgggca gtggcagcag gcgccccttc
     8041 cccagcagca gcccatgcca ggcttgccca ggcctgtgat atccatgcag gcccaggcgg
     8101 ccgtggctgg gccccggatg cccagcgtgc agccacccag gagcatctca cccagcgctc
     8161 tgcaagacct gctgcggacc ctgaagtcgc ccagctcccc tcagcagcaa cagcaggtgc
     8221 tgaacattct caaatcaaac ccgcagctaa tggcagcttt catcaaacag cgcacagcca
     8281 agtacgtggc caatcagccc ggcatgcagc cccagcctgg cctccagtcc cagcccggca
     8341 tgcaacccca gcctggcatg caccagcagc ccagcctgca gaacctgaat gccatgcagg
     8401 ctggcgtgcc gcggcccggt gtgcctccac agcagcaggc gatgggaggc ctgaaccccc
     8461 agggccaggc cttgaacatc atgaacccag gacacaaccc caacatggcg agtatgaatc
     8521 cacagtaccg agaaatgtta cggaggcagc tgctgcagca gcagcagcaa cagcagcagc
     8581 aacaacagca gcaacagcag cagcagcaag ggagtgccgg catggctggg ggcatggcgg
     8641 ggcacggcca gttccagcag cctcaaggac ccggaggcta cccaccggcc atgcagcagc
     8701 agcagcgcat gcagcagcat ctccccctcc agggcagctc catgggccag atggcggctc
     8761 agatgggaca gcttggccag atggggcagc cggggctggg ggcagacagc acccccaaca
     8821 tccagcaagc cctgcagcag cggattctgc agcaacagca gatgaagcag cagattgggt
     8881 ccccaggcca gccgaacccc atgagccccc agcaacacat gctctcagga cagccacagg
     8941 cctcgcatct ccctggccag cagatcgcca cgtcccttag taaccaggtg cggtctccag
     9001 cccctgtcca gtctccacgg ccccagtccc agcctccaca ttccagcccg tcaccacgga
     9061 tacagcccca gccttcgcca caccacgtct caccccagac tggttccccc caccccggac
     9121 tcgcagtcac catggccagc tccatagatc agggacactt ggggaacccc gaacagagtg
     9181 caatgctccc ccagctgaac acccccagca ggagtgcgct gtccagcgaa ctgtccctgg
     9241 tcggggacac cacgggggac acgctagaga agtttgtgga gggcttgccg gtcatggtga
     9301 gcaagggcgc cgagctgttc accggcatcg tgcccatcct gatcgagctg aatggcgatg
     9361 tgaatggcca caagttcagc gtgagcggcg agggcgaggg cgatgccacc tacggcaagc
     9421 tgaccctgaa gttcatctgc accaccggca agctgcctgt gccctggccc accctggtga
     9481 ccaccctgag ctacggcgtg cagtgcttct cacgctaccc cgatcacatg aagcagcacg
     9541 acttcttcaa gagcgccatg cctgagggct acatccagga gcgcaccatc ttcttcgagg
     9601 atgacggcaa ctacaagtcg cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc
     9661 gcatcgagct gaccggcacc gatttcaagg aggatggcaa catcctgggc aataagatgg
     9721 agtacaacta caacgcccac aatgtgtaca tcatgaccga caaggccaag aatggcatca
     9781 aggtgaactt caagatccgc cacaacatcg aggatggcag cgtgcagctg gccgaccact
     9841 accagcagaa tacccccatc ggcgatggcc ctgtgctgct gcccgataac cactacctgt
     9901 ccacccagag cgccctgtcc aaggacccca acgagaagcg cgatcacatg atctacttcg
     9961 gcttcgtgac cgccgccgcc atcacccacg gcatggatga gctgtacaag tgagcggccg
    10021 cgccggcggg cccgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
    10081 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
    10141 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    10201 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    10261 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    10321 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    10381 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
    10441 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
    10501 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
    10561 accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
    10621 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
    10681 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
    10741 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
    10801 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
    10861 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
    10921 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
    10981 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
    11041 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
    11101 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
    11161 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
    11221 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
    11281 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
    11341 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
    11401 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
    11461 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
    11521 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
    11581 aaggaatagc gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
    11641 gcatcggcgt ctcgagatat cagtggtcca ggctctagtt ttgactcaac aatatcacca
    11701 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa
    11761 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
    11821 ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac
    11881 agatggaaca gggtcgaccc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
    11941 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
    12001 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
    12061 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
    12121 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
    12181 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
    12241 acccaccacc gggaggtaag ctggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
    12301 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    12361 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
    12421 gtcacgtagc gatagcggag tgtagatccg gctgtggaat gtgtgtcagt tagggtgtgg
    12481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    12541 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    12601 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    12661 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    12721 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    12781 cttttgcaaa aagcttactg gcttaactat gcggcatcag agcagattgt actgagagtg
    12841 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    12901 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    12961 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    13021 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    13081 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    13141 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    13201 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    13261 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    13321 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    13381 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    13441 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    13501 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    13561 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    13621 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    13681 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    13741 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    13801 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    13861 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    13921 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    13981 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    14041 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    14101 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
    14161 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    14221 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    14281 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    14341 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    14401 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
    14461 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    14521 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    14581 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    14641 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    14701 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    14761 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    14821 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    14881 cgtatcacga ggccctttcg tcttcaagaa ttagcttggc cattgcatac gttgtatcca
    14941 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
    15001 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
    15061 gagttc
//
