LOCUS       sequence-437453-       11347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaIDR6KTG in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11347)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdIDR6KTG
FEATURES             Location/Qualifiers
     source          1..11347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5533..5542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7294..8013
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    8135..8256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8172..8191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8226..8245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8263..8718)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8400..8421)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8612..8631
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8745..8849
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8851..9208
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8851..9042
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8874..8894)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9059..9194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9243..10037
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9243..10037
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9297..9316)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9907..9926
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10225..10244)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10269..10316
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10645..11233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11134..11153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgaaacc aacaaaccat cctggggtct ccagctagtg gaattcaaaa cacaattggt
     2041 tctgttggca cagggcaaca gaatgccact tctttaagta acccaaatcc catagacccc
     2101 agctccatgc agcgagccta tgctgctctc ggactcccct acatgaacca gccccagacg
     2161 cagctgcagc ctcaggttcc tggccagcaa ccagcacagc ctcaaaccca ccagcagatg
     2221 aggactctca accccctggg aaataatcca atgaacattc cagcaggagg aataacaaca
     2281 gatcagcagc ccccaaactt gatttcagaa tcagctcttc cgacttccct gggggccaca
     2341 aacccactga tgaacgatgg ctccaactct ggtaacattg gaaccctcag cactatacca
     2401 acagcagctc ctccttctag caccggtgta aggaaaggct ggcacgaaca tgtcactcag
     2461 gacctgcgga gccatctagt gcataaactc gtccaagcca tcttcccaac acctgatccc
     2521 gcagctctaa aggatcgccg catggaaaac ctggtagcct atgctaagaa agtggaaggg
     2581 gacatgtacg agtctgccaa cagcagggat gaatattatc acttattagc agagaaaatc
     2641 tacaagatac aaaaagaact agaagaaaaa cggaggtcgc gtttacataa acaaggcatc
     2701 ttggggaacc agccagcctt accagccccg ggggctcagc cccctgtgat tccacaggca
     2761 caacctgtga gacctccaaa tggacccctg tccctgccag tgaatcgcat gcaagtttct
     2821 caagggatga attcatttaa ccccatgtcc ttggggaacg tccagttgcc acaagcaccc
     2881 atgggacctc gtgcagcctc cccaatgaac cactctgtcc agatgaacag catgggctca
     2941 gtgccaggga tggccatttc tccttcccga atgcctcagc ctccgaacat gatgggtgca
     3001 cacaccaaca acatgatggc ccaggcgccc gctcagagcc agtttctgcc acagaaccag
     3061 ttcccgtcat ccagcggggc gatgagtgtg ggcatggggc agccgccagc ccaaacaggc
     3121 gtgtcacagg gacaggtgcc tggtgctgct cttcctaacc ctctcaacat gctggggcct
     3181 caggccagcc agctaccttg ccctccagtg acacagtcac cactgcaccc aacaccgcct
     3241 cctgcttcca cggctgctgg catgccatct ctccagcaca cgacaccacc tgggatgact
     3301 cctccccagc cagcagctcc cactcagcca tcaactcctg tgtcgtcttc cgggcagact
     3361 cccaccccga ctcctggctc agtgcccagt gctacccaaa cccagagcac ccctacagtc
     3421 caggcagcag cccaggccca ggtgaccccg cagcctcaaa ccccagttca gcccccgtct
     3481 gtggctaccc ctcagtcatc gcagcaacag ccgacgcctg tgcacgccca gcctcctggc
     3541 acaccgcttt cccaggcagc agccagcatt gataacagag tccctacccc ctcctcggtg
     3601 gccagcgcag aaaccaattc ccagcagcca ggacctgacg tacctgtgct ggaaatgaag
     3661 acggagaccc aagcagagga cactgagccc gatcctggtg aatccaaagg ggagcccagg
     3721 tctgagatga tggaggagga tttgcaagga gcttcccaag ttaaagaaga aacagacata
     3781 gcagagcaga aatcagaacc aatggaagtg gatgaaaaga aacctgaagt gaaagtagaa
     3841 gttaaagagg aagaagagag tagcagtaac ggcacagcct ctcagtcaac atctccttcg
     3901 cagccgcgca aaaaaatctt taaaccagag gagttacgcc aggccctcat gccaacccta
     3961 gaagcactgt atcgacagga cccagagtca ttacctttcc ggcagcctgt agatccccag
     4021 ctcctcggaa ttccagacta ttttgacatc gtaaagaatc ccatggacct ctccaccatc
     4081 aagcggaagc tggacacagg gcaataccaa gagccctggc agtacgtgga cgacgtctgg
     4141 ctcatgttca acaatgcctg gctctataat cgcaagacat cccgagtcta taagttttgc
     4201 agtaagcttg cagaggtctt tgagcaggaa attgaccctg tcatgcagtc ccttggatat
     4261 tgctgtggac gcaagtatga gttttcccca cagactttgt gctgctatgg gaagcagctg
     4321 tgtaccattc ctcgcgatgc tgcctactac agctatcaga ataggtatca tttctgtgag
     4381 aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc ttcacagccc
     4441 cagacgacaa tttcaaagga tcagtttgaa aagaagaaaa atgatacctt agaccccgaa
     4501 cctttcgttg attgcaagga gtgtggccgg aagatgcatc agatttgcgt tctgcactat
     4561 gacatcattt ggccttcagg ttttgtgtgc gacaactgct tgaagaaaac tggcagacct
     4621 cgaaaagaaa acaaattcag tgctaagagg ctgcagacca caagactggg aaaccacttg
     4681 gaagaccgag tgaacaaatt tttgcggcgc cagaatcacc ctgaagccgg ggaggttttt
     4741 gtccgagtgg tggccagctc agacaagacg gtggaggtca agcccgggat gaagtcacgg
     4801 tttgtggatt ctggggaaat gtctgaatct ttcccatatc gaaccaaagc tctgtttgct
     4861 tttgaggaaa ttgacggcgt ggatgtctgc ttttttggaa tgcacgtcca agaatacggc
     4921 tctgattgcc cccctccaaa cacgaggcgt gtgtacattt cttatctgga tagtattcat
     4981 ttcttccggc cacgttgcct ccgcacagcc gtttaccatg agatccttat tggatattta
     5041 gagtatgtga agaaattagg gtatgtgaca gggcacatct gggcctgtcc tccaagtgaa
     5101 ggagatgatt acatcttcca ttgccaccca cctgatcaaa aaatacccaa gccaaaacga
     5161 ctgcaggagt ggtacaaaaa gatgctggac aaggcgtttg cagagcggat catccatgac
     5221 tacaaggata ttttcaaaca agcaactgaa gacaggctca ccagtgccaa ggaactgccc
     5281 tattttgaag gtgatttctg gcccaatgtg ttagaagaga gcattaagga actagaacaa
     5341 gaagaagagg agaggggtgg cgaagagagc actgcagcca gtgaaaccac tgagggcagt
     5401 cagggcgaca gcgggaatgc cggcggtggg aacaacggcg gtaccaacgg gaacggtagc
     5461 agcatcagcc gcgccaacgg cggtgggccc agcatgccca acgtgtccaa tgacctgtcc
     5521 cagaagctgt atgccaccat ggagaagcac aaggaggtct tcttcgtgat ccacctgcac
     5581 gctgggcctg tcatcaacac cctgcccccc atcgtcgacc ccgaccccct gctcagctgt
     5641 gacctcatgg atgggcgcga cgccttcctc accctcgcca gagacaagca ctgggagttc
     5701 tcctccttgc gccgctccaa gtggtccacg ctctgcatgc tggtggagct gcacacccag
     5761 ggccaggacc gctttgtcta cacctgcaac gagtgcaagc accacgtgga gacgcgctgg
     5821 cactgcactg tgtgcgagga ctacgacctc tgcatcaact gctataacac gaagagccat
     5881 gcccataaga tggtgaagtg ggggctgggc ctggatgacg agggcagcag ccagggcgag
     5941 ccacagtcaa agagccccca ggagtcacgc cggctgagca tccagcgctg catccagtcg
     6001 ctggtgcacg cgtgccagtg ccgcaacgcc aactgctcgc tgccatcctg ccagaagatg
     6061 aagcgggtgg tgcagcacac caagggctgc aaacgcaaga ccaacggggg ctgcccggtg
     6121 tgcaagcagc tcatcgccct ctgctgctac cacgccaagc actgccaaga aaacaaatgc
     6181 cccgtgccct tctgcctcaa catcaaacac aagctccgcc agcagcagat caaatcaaac
     6241 ccgcagctaa tggcagcttt catcaaacag cgcacagcca agtacgtggc caatcagccc
     6301 ggcatgcagc cccagcctgg cctccagtcc cagcccggca tgcaacccca gcctggcatg
     6361 caccagcagc ccagcctgca gaacctgaat gccatgcagg ctggcgtgcc gcggcccggt
     6421 gtgcctccac agcagcaggc gatgggaggc ctgaaccccc agggccaggc cttgaacatc
     6481 atgaacccag gacacaaccc caacatggcg agtatgaatc cacagtaccg agaaatgtta
     6541 cggaggcagc tgctgcagca gcagcagcaa cagcagcagc aacaacagca gcaacagcag
     6601 cagcagcaag ggagtgccgg catggctggg ggcatggcgg ggcacggcca gttccagcag
     6661 cctcaaggac ccggaggcta cccaccggcc atgcagcagc agcagcgcat gcagcagcat
     6721 ctccccctcc agggcagctc catgggccag atggcggctc agatgggaca gcttggccag
     6781 atggggcagc cggggctggg ggcagacagc acccccaaca tccagcaagc cctgcagcag
     6841 cggattctgc agcaacagca gatgaagcag cagattgggt ccccaggcca gccgaacccc
     6901 atgagccccc agcaacacat gctctcagga cagccacagg cctcgcatct ccctggccag
     6961 cagatcgcca cgtcccttag taaccaggtg cggtctccag cccctgtcca gtctccacgg
     7021 ccccagtccc agcctccaca ttccagcccg tcaccacgga tacagcccca gccttcgcca
     7081 caccacgtct caccccagac tggttccccc caccccggac tcgcagtcac catggccagc
     7141 tccatagatc agggacactt ggggaacccc gaacagagtg caatgctccc ccagctgaac
     7201 acccccagca ggagtgcgct gtccagcgaa ctgtccctgg tcggggacac cacgggggac
     7261 acgctagaga agtttgtgga gggcttgccg gtcatggtga gcaagggcgc cgagctgttc
     7321 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc
     7381 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     7441 accaccggca agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg
     7501 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg
     7561 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg
     7621 cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc
     7681 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac
     7741 aatgtgtaca tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc
     7801 cacaacatcg aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc
     7861 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc
     7921 aaggacccca acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc
     7981 atcacccacg gcatggatga gctgtacaag tgagcggccg cgactctaga tcataatcag
     8041 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     8101 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     8161 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8221 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     8281 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     8341 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     8401 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     8461 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     8521 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     8581 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     8641 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     8701 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8761 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8821 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     8881 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8941 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9001 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9061 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9121 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9181 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     9241 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     9301 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     9361 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     9421 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     9481 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     9541 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     9601 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     9661 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     9721 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     9781 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     9841 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     9901 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     9961 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    10021 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
    10081 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    10141 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    10201 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
    10261 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
    10321 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
    10381 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
    10441 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
    10501 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10561 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10621 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10681 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10741 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10801 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10861 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10921 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10981 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    11041 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    11101 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    11161 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11221 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    11281 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    11341 catgcat
//
