LOCUS       sequence-437454-       11233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaIDR6deltaAL in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11233)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdIDR6dAL
FEATURES             Location/Qualifiers
     source          1..11233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5419..5428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7180..7899
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    8021..8142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8058..8077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8112..8131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8149..8604)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8286..8307)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8498..8517
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8631..8735
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8737..9094
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8737..8928
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8760..8780)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8945..9080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9129..9923
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9129..9923
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9183..9202)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9793..9812
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10111..10130)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10155..10202
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10531..11119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11020..11039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgaaacc aacaaaccat cctggggtct ccagctagtg gaattcaaaa cacaattggt
     2041 tctgttggca cagggcaaca gaatgccact tctttaagta acccaaatcc catagacccc
     2101 agctccatgc agcgagccta tgctgctctc ggactcccct acatgaacca gccccagacg
     2161 cagctgcagc ctcaggttcc tggccagcaa ccagcacagc ctcaaaccca ccagcagatg
     2221 aggactctca accccctggg aaataatcca atgaacattc cagcaggagg aataacaaca
     2281 gatcagcagc ccccaaactt gatttcagaa tcagctcttc cgacttccct gggggccaca
     2341 aacccactga tgaacgatgg ctccaactct ggtaacattg gaaccctcag cactatacca
     2401 acagcagctc ctccttctag caccggtgta aggaaaggct ggcacgaaca tgtcactcag
     2461 gacctgcgga gccatctagt gcataaactc gtccaagcca tcttcccaac acctgatccc
     2521 gcagctctaa aggatcgccg catggaaaac ctggtagcct atgctaagaa agtggaaggg
     2581 gacatgtacg agtctgccaa cagcagggat gaatattatc acttattagc agagaaaatc
     2641 tacaagatac aaaaagaact agaagaaaaa cggaggtcgc gtttacataa acaaggcatc
     2701 ttggggaacc agccagcctt accagccccg ggggctcagc cccctgtgat tccacaggca
     2761 caacctgtga gacctccaaa tggacccctg tccctgccag tgaatcgcat gcaagtttct
     2821 caagggatga attcatttaa ccccatgtcc ttggggaacg tccagttgcc acaagcaccc
     2881 atgggacctc gtgcagcctc cccaatgaac cactctgtcc agatgaacag catgggctca
     2941 gtgccaggga tggccatttc tccttcccga atgcctcagc ctccgaacat gatgggtgca
     3001 cacaccaaca acatgatggc ccaggcgccc gctcagagcc agtttctgcc acagaaccag
     3061 ttcccgtcat ccagcggggc gatgagtgtg ggcatggggc agccgccagc ccaaacaggc
     3121 gtgtcacagg gacaggtgcc tggtgctgct cttcctaacc ctctcaacat gctggggcct
     3181 caggccagcc agctaccttg ccctccagtg acacagtcac cactgcaccc aacaccgcct
     3241 cctgcttcca cggctgctgg catgccatct ctccagcaca cgacaccacc tgggatgact
     3301 cctccccagc cagcagctcc cactcagcca tcaactcctg tgtcgtcttc cgggcagact
     3361 cccaccccga ctcctggctc agtgcccagt gctacccaaa cccagagcac ccctacagtc
     3421 caggcagcag cccaggccca ggtgaccccg cagcctcaaa ccccagttca gcccccgtct
     3481 gtggctaccc ctcagtcatc gcagcaacag ccgacgcctg tgcacgccca gcctcctggc
     3541 acaccgcttt cccaggcagc agccagcatt gataacagag tccctacccc ctcctcggtg
     3601 gccagcgcag aaaccaattc ccagcagcca ggacctgacg tacctgtgct ggaaatgaag
     3661 acggagaccc aagcagagga cactgagccc gatcctggtg aatccaaagg ggagcccagg
     3721 tctgagatga tggaggagga tttgcaagga gcttcccaag ttaaagaaga aacagacata
     3781 gcagagcaga aatcagaacc aatggaagtg gatgaaaaga aacctgaagt gaaagtagaa
     3841 gttaaagagg aagaagagag tagcagtaac ggcacagcct ctcagtcaac atctccttcg
     3901 cagccgcgca aaaaaatctt taaaccagag gagttacgcc aggccctcat gccaacccta
     3961 gaagcactgt atcgacagga cccagagtca ttacctttcc ggcagcctgt agatccccag
     4021 ctcctcggaa ttccagacta ttttgacatc gtaaagaatc ccatggacct ctccaccatc
     4081 aagcggaagc tggacacagg gcaataccaa gagccctggc agtacgtgga cgacgtctgg
     4141 ctcatgttca acaatgcctg gctctataat cgcaagacat cccgagtcta taagttttgc
     4201 agtaagcttg cagaggtctt tgagcaggaa attgaccctg tcatgcagtc ccttggatat
     4261 tgctgtggac gcaagtatga gttttcccca cagactttgt gctgctatgg gaagcagctg
     4321 tgtaccattc ctcgcgatgc tgcctactac agctatcaga ataggtatca tttctgtgag
     4381 aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc ttcacagccc
     4441 cagacgacaa tttcaaagga tcagtttgaa aagaagaaaa atgatacctt agaccccgaa
     4501 cctttcgttg attgcaagga gtgtggccgg aagatgcatc agatttgcgt tctgcactat
     4561 gacatcattt ggccttcagg ttttgtgtgc gacaactgct tgaagaaaac tggcagacct
     4621 cgaaaagaaa acaaattcag tgctaagagg ctgcagacca caagactggg aaaccacttg
     4681 gaagaccgag tgaacaaatt tttgcggcgc cagaatcacc ctgaagccgg ggaggttttt
     4741 gtccgagtgg tggccagctc agacaagacg gtggaggtca agcccgggat gaagtcacgg
     4801 tttgtggatt ctggggaaat gtctgaatct ttcccatatc gaaccaaagc tctgtttgct
     4861 tttgaggaaa ttgacggcgt ggatgtctgc ttttttggaa tgcacgtcca agaatacggc
     4921 tctgattgcc cccctccaaa cacgaggcgt gtgtacattt cttatctgga tagtattcat
     4981 ttcttccggc cacgttgcct ccgcacagcc gtttaccatg agatccttat tggatattta
     5041 gagtatgtga agaaattagg gtatgtgaca gggcacatct gggcctgtcc tccaagtgaa
     5101 ggagatgatt acatcttcca ttgccaccca cctgatcaaa aaatacccaa gccaaaacga
     5161 ctgcaggagt ggtacaaaaa gatgctggac aaggcgtttg cagagcggat catccatgac
     5221 tacaaggata ttttcaaaca agcaactgaa gacaggctca ccagtgccaa ggaactgccc
     5281 tattttgaag gtgatttctg gcccaatgtg ttagaagaga gcattaagga actagaaggc
     5341 agtgccggga gcgccgcagg gagtggtgaa tttcccagca tgcccaacgt gtccaatgac
     5401 ctgtcccaga agctgtatgc caccatggag aagcacaagg aggtcttctt cgtgatccac
     5461 ctgcacgctg ggcctgtcat caacaccctg ccccccatcg tcgaccccga ccccctgctc
     5521 agctgtgacc tcatggatgg gcgcgacgcc ttcctcaccc tcgccagaga caagcactgg
     5581 gagttctcct ccttgcgccg ctccaagtgg tccacgctct gcatgctggt ggagctgcac
     5641 acccagggcc aggaccgctt tgtctacacc tgcaacgagt gcaagcacca cgtggagacg
     5701 cgctggcact gcactgtgtg cgaggactac gacctctgca tcaactgcta taacacgaag
     5761 agccatgccc ataagatggt gaagtggggg ctgggcctgg atgacgaggg cagcagccag
     5821 ggcgagccac agtcaaagag cccccaggag tcacgccggc tgagcatcca gcgctgcatc
     5881 cagtcgctgg tgcacgcgtg ccagtgccgc aacgccaact gctcgctgcc atcctgccag
     5941 aagatgaagc gggtggtgca gcacaccaag ggctgcaaac gcaagaccaa cgggggctgc
     6001 ccggtgtgca agcagctcat cgccctctgc tgctaccacg ccaagcactg ccaagaaaac
     6061 aaatgccccg tgcccttctg cctcaacatc aaacacaagc tccgccagca gcagatcaaa
     6121 tcaaacccgc agctaatggc agctttcatc aaacagcgca cagccaagta cgtggccaat
     6181 cagcccggca tgcagcccca gcctggcctc cagtcccagc ccggcatgca accccagcct
     6241 ggcatgcacc agcagcccag cctgcagaac ctgaatgcca tgcaggctgg cgtgccgcgg
     6301 cccggtgtgc ctccacagca gcaggcgatg ggaggcctga acccccaggg ccaggccttg
     6361 aacatcatga acccaggaca caaccccaac atggcgagta tgaatccaca gtaccgagaa
     6421 atgttacgga ggcagctgct gcagcagcag cagcaacagc agcagcaaca acagcagcaa
     6481 cagcagcagc agcaagggag tgccggcatg gctgggggca tggcggggca cggccagttc
     6541 cagcagcctc aaggacccgg aggctaccca ccggccatgc agcagcagca gcgcatgcag
     6601 cagcatctcc ccctccaggg cagctccatg ggccagatgg cggctcagat gggacagctt
     6661 ggccagatgg ggcagccggg gctgggggca gacagcaccc ccaacatcca gcaagccctg
     6721 cagcagcgga ttctgcagca acagcagatg aagcagcaga ttgggtcccc aggccagccg
     6781 aaccccatga gcccccagca acacatgctc tcaggacagc cacaggcctc gcatctccct
     6841 ggccagcaga tcgccacgtc ccttagtaac caggtgcggt ctccagcccc tgtccagtct
     6901 ccacggcccc agtcccagcc tccacattcc agcccgtcac cacggataca gccccagcct
     6961 tcgccacacc acgtctcacc ccagactggt tccccccacc ccggactcgc agtcaccatg
     7021 gccagctcca tagatcaggg acacttgggg aaccccgaac agagtgcaat gctcccccag
     7081 ctgaacaccc ccagcaggag tgcgctgtcc agcgaactgt ccctggtcgg ggacaccacg
     7141 ggggacacgc tagagaagtt tgtggagggc ttgccggtca tggtgagcaa gggcgccgag
     7201 ctgttcaccg gcatcgtgcc catcctgatc gagctgaatg gcgatgtgaa tggccacaag
     7261 ttcagcgtga gcggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     7321 atctgcacca ccggcaagct gcctgtgccc tggcccaccc tggtgaccac cctgagctac
     7381 ggcgtgcagt gcttctcacg ctaccccgat cacatgaagc agcacgactt cttcaagagc
     7441 gccatgcctg agggctacat ccaggagcgc accatcttct tcgaggatga cggcaactac
     7501 aagtcgcgcg ccgaggtgaa gttcgagggc gataccctgg tgaatcgcat cgagctgacc
     7561 ggcaccgatt tcaaggagga tggcaacatc ctgggcaata agatggagta caactacaac
     7621 gcccacaatg tgtacatcat gaccgacaag gccaagaatg gcatcaaggt gaacttcaag
     7681 atccgccaca acatcgagga tggcagcgtg cagctggccg accactacca gcagaatacc
     7741 cccatcggcg atggccctgt gctgctgccc gataaccact acctgtccac ccagagcgcc
     7801 ctgtccaagg accccaacga gaagcgcgat cacatgatct acttcggctt cgtgaccgcc
     7861 gccgccatca cccacggcat ggatgagctg tacaagtgag cggccgcgac tctagatcat
     7921 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7981 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     8041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8101 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     8161 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     8221 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     8281 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     8341 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     8401 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     8461 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     8521 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     8581 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8641 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8701 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     8761 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8821 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8881 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8941 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9001 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9061 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     9121 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     9181 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     9241 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     9301 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     9361 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     9421 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9481 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9541 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9601 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     9661 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9721 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9781 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9841 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9901 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     9961 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
    10021 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
    10081 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
    10141 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
    10201 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
    10261 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
    10321 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
    10381 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10441 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    11041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    11161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    11221 taccgccatg cat
//
