LOCUS       sequence-437455-       11968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaIDR6-H2 in mammalian 
            cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11968)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdIDR6-H2
FEATURES             Location/Qualifiers
     source          1..11968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5533..5542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7915..8634
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    8756..8877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8793..8812)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8847..8866
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8884..9339)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9021..9042)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9233..9252
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9366..9470
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9472..9829
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9472..9663
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9495..9515)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9680..9815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9864..10658
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9864..10658
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9918..9937)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10528..10547
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10846..10865)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10890..10937
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11266..11854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11755..11774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgaaacc aacaaaccat cctggggtct ccagctagtg gaattcaaaa cacaattggt
     2041 tctgttggca cagggcaaca gaatgccact tctttaagta acccaaatcc catagacccc
     2101 agctccatgc agcgagccta tgctgctctc ggactcccct acatgaacca gccccagacg
     2161 cagctgcagc ctcaggttcc tggccagcaa ccagcacagc ctcaaaccca ccagcagatg
     2221 aggactctca accccctggg aaataatcca atgaacattc cagcaggagg aataacaaca
     2281 gatcagcagc ccccaaactt gatttcagaa tcagctcttc cgacttccct gggggccaca
     2341 aacccactga tgaacgatgg ctccaactct ggtaacattg gaaccctcag cactatacca
     2401 acagcagctc ctccttctag caccggtgta aggaaaggct ggcacgaaca tgtcactcag
     2461 gacctgcgga gccatctagt gcataaactc gtccaagcca tcttcccaac acctgatccc
     2521 gcagctctaa aggatcgccg catggaaaac ctggtagcct atgctaagaa agtggaaggg
     2581 gacatgtacg agtctgccaa cagcagggat gaatattatc acttattagc agagaaaatc
     2641 tacaagatac aaaaagaact agaagaaaaa cggaggtcgc gtttacataa acaaggcatc
     2701 ttggggaacc agccagcctt accagccccg ggggctcagc cccctgtgat tccacaggca
     2761 caacctgtga gacctccaaa tggacccctg tccctgccag tgaatcgcat gcaagtttct
     2821 caagggatga attcatttaa ccccatgtcc ttggggaacg tccagttgcc acaagcaccc
     2881 atgggacctc gtgcagcctc cccaatgaac cactctgtcc agatgaacag catgggctca
     2941 gtgccaggga tggccatttc tccttcccga atgcctcagc ctccgaacat gatgggtgca
     3001 cacaccaaca acatgatggc ccaggcgccc gctcagagcc agtttctgcc acagaaccag
     3061 ttcccgtcat ccagcggggc gatgagtgtg ggcatggggc agccgccagc ccaaacaggc
     3121 gtgtcacagg gacaggtgcc tggtgctgct cttcctaacc ctctcaacat gctggggcct
     3181 caggccagcc agctaccttg ccctccagtg acacagtcac cactgcaccc aacaccgcct
     3241 cctgcttcca cggctgctgg catgccatct ctccagcaca cgacaccacc tgggatgact
     3301 cctccccagc cagcagctcc cactcagcca tcaactcctg tgtcgtcttc cgggcagact
     3361 cccaccccga ctcctggctc agtgcccagt gctacccaaa cccagagcac ccctacagtc
     3421 caggcagcag cccaggccca ggtgaccccg cagcctcaaa ccccagttca gcccccgtct
     3481 gtggctaccc ctcagtcatc gcagcaacag ccgacgcctg tgcacgccca gcctcctggc
     3541 acaccgcttt cccaggcagc agccagcatt gataacagag tccctacccc ctcctcggtg
     3601 gccagcgcag aaaccaattc ccagcagcca ggacctgacg tacctgtgct ggaaatgaag
     3661 acggagaccc aagcagagga cactgagccc gatcctggtg aatccaaagg ggagcccagg
     3721 tctgagatga tggaggagga tttgcaagga gcttcccaag ttaaagaaga aacagacata
     3781 gcagagcaga aatcagaacc aatggaagtg gatgaaaaga aacctgaagt gaaagtagaa
     3841 gttaaagagg aagaagagag tagcagtaac ggcacagcct ctcagtcaac atctccttcg
     3901 cagccgcgca aaaaaatctt taaaccagag gagttacgcc aggccctcat gccaacccta
     3961 gaagcactgt atcgacagga cccagagtca ttacctttcc ggcagcctgt agatccccag
     4021 ctcctcggaa ttccagacta ttttgacatc gtaaagaatc ccatggacct ctccaccatc
     4081 aagcggaagc tggacacagg gcaataccaa gagccctggc agtacgtgga cgacgtctgg
     4141 ctcatgttca acaatgcctg gctctataat cgcaagacat cccgagtcta taagttttgc
     4201 agtaagcttg cagaggtctt tgagcaggaa attgaccctg tcatgcagtc ccttggatat
     4261 tgctgtggac gcaagtatga gttttcccca cagactttgt gctgctatgg gaagcagctg
     4321 tgtaccattc ctcgcgatgc tgcctactac agctatcaga ataggtatca tttctgtgag
     4381 aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc ttcacagccc
     4441 cagacgacaa tttcaaagga tcagtttgaa aagaagaaaa atgatacctt agaccccgaa
     4501 cctttcgttg attgcaagga gtgtggccgg aagatgcatc agatttgcgt tctgcactat
     4561 gacatcattt ggccttcagg ttttgtgtgc gacaactgct tgaagaaaac tggcagacct
     4621 cgaaaagaaa acaaattcag tgctaagagg ctgcagacca caagactggg aaaccacttg
     4681 gaagaccgag tgaacaaatt tttgcggcgc cagaatcacc ctgaagccgg ggaggttttt
     4741 gtccgagtgg tggccagctc agacaagacg gtggaggtca agcccgggat gaagtcacgg
     4801 tttgtggatt ctggggaaat gtctgaatct ttcccatatc gaaccaaagc tctgtttgct
     4861 tttgaggaaa ttgacggcgt ggatgtctgc ttttttggaa tgcacgtcca agaatacggc
     4921 tctgattgcc cccctccaaa cacgaggcgt gtgtacattt cttatctgga tagtattcat
     4981 ttcttccggc cacgttgcct ccgcacagcc gtttaccatg agatccttat tggatattta
     5041 gagtatgtga agaaattagg gtatgtgaca gggcacatct gggcctgtcc tccaagtgaa
     5101 ggagatgatt acatcttcca ttgccaccca cctgatcaaa aaatacccaa gccaaaacga
     5161 ctgcaggagt ggtacaaaaa gatgctggac aaggcgtttg cagagcggat catccatgac
     5221 tacaaggata ttttcaaaca agcaactgaa gacaggctca ccagtgccaa ggaactgccc
     5281 tattttgaag gtgatttctg gcccaatgtg ttagaagaga gcattaagga actagaacaa
     5341 gaagaagagg agaggaaaaa ggaagagagc actgcagcca gtgaaaccac tgagggcagt
     5401 cagggcgaca gcaagaatgc caagaagaag aacaacaaga aaaccaacaa gaacaaaagc
     5461 agcatcagcc gcgccaacaa gaagaagccc agcatgccca acgtgtccaa tgacctgtcc
     5521 cagaagctgt atgccaccat ggagaagcac aaggaggtct tcttcgtgat ccacctgcac
     5581 gctgggcctg tcatcaacac cctgcccccc atcgtcgacc ccgaccccct gctcagctgt
     5641 gacctcatgg atgggcgcga cgccttcctc accctcgcca gagacaagca ctgggagttc
     5701 tcctccttgc gccgctccaa gtggtccacg ctctgcatgc tggtggagct gcacacccag
     5761 ggccaggacc gctttgtcta cacctgcaac gagtgcaagc accacgtgga gacgcgctgg
     5821 cactgcactg tgtgcgagga ctacgacctc tgcatcaact gctataacac gaagagccat
     5881 gcccataaga tggtgaagtg ggggctgggc ctggatgacg agggcagcag ccagggcgag
     5941 ccacagtcaa agagccccca ggagtcacgc cggctgagca tccagcgctg catccagtcg
     6001 ctggtgcacg cgtgccagtg ccgcaacgcc aactgctcgc tgccatcctg ccagaagatg
     6061 aagcgggtgg tgcagcacac caagggctgc aaacgcaaga ccaacggggg ctgcccggtg
     6121 tgcaagcagc tcatcgccct ctgctgctac cacgccaagc actgccaaga aaacaaatgc
     6181 cccgtgccct tctgcctcaa catcaaacac aagctccgcc agcagcagat ccagcaccgc
     6241 ctgcagcagg cccagctcat gcgccggcgg atggccacca tgaacacccg caacgtgcct
     6301 cagcagagtc tgccttctcc tacctcagca ccgcccggga cccccacaca gcagcccagc
     6361 acaccccaga cgccgcagcc ccctgcccag ccccaaccct cacccgtgag catgtcacca
     6421 gctggcttcc ccagcgtggc ccggactcag ccccccacca cggtgtccac agggaagcct
     6481 accagccagg tgccggcccc cccacccccg gcccagaaca gcatgccccc aggacgcacg
     6541 ggcatgggga ccccggggag ccagatggcc cccgtgagcc tgaatgtgcc ccgacccaac
     6601 caggtgagcg ggcccgtcat gcccagcatg cctcccgggc agtggcagca ggcgcccctt
     6661 ccccagcagc agcccatgcc aggcttgccc aggcctgtga tatccatgca ggcccaggcg
     6721 gccgtggctg ggccccggat gcccagcgtg cagccaccca ggagcatctc acccagcgct
     6781 ctgcaagacc tgctgcggac cctgaagtcg cccagctccc ctcagcagca acagcaggtg
     6841 ctgaacattc tcaaatcaaa cccgcagcta atggcagctt tcatcaaaca gcgcacagcc
     6901 aagtacgtgg ccaatcagcc cggcatgcag ccccagcctg gcctccagtc ccagcccggc
     6961 atgcaacccc agcctggcat gcaccagcag cccagcctgc agaacctgaa tgccatgcag
     7021 gctggcgtgc cgcggcccgg tgtgcctcca cagcagcagg cgatgggagg cctgaacccc
     7081 cagggccagg ccttgaacat catgaaccca ggacacaacc ccaacatggc gagtatgaat
     7141 ccacagtacc gagaaatgtt acggaggcag ctgctgcagc agcagcagca acagcagcag
     7201 caacaacagc agcaacagca gcagcagcaa gggagtgccg gcatggctgg gggcatggcg
     7261 gggcacggcc agttccagca gcctcaagga cccggaggct acccaccggc catgcagcag
     7321 cagcagcgca tgcagcagca tctccccctc cagggcagct ccatgggcca gatggcggct
     7381 cagatgggac agcttggcca gatggggcag ccggggctgg gggcagacag cacccccaac
     7441 atccagcaag ccctgcagca gcggattctg cagcaacagc agatgaagca gcagattggg
     7501 tccccaggcc agccgaaccc catgagcccc cagcaacaca tgctctcagg acagccacag
     7561 gcctcgcatc tccctggcca gcagatcgcc acgtccctta gtaaccaggt gcggtctcca
     7621 gcccctgtcc agtctccacg gccccagtcc cagcctccac attccagccc gtcaccacgg
     7681 atacagcccc agccttcgcc acaccacgtc tcaccccaga ctggttcccc ccaccccgga
     7741 ctcgcagtca ccatggccag ctccatagat cagggacact tggggaaccc cgaacagagt
     7801 gcaatgctcc cccagctgaa cacccccagc aggagtgcgc tgtccagcga actgtccctg
     7861 gtcggggaca ccacggggga cacgctagag aagtttgtgg agggcttgcc ggtcatggtg
     7921 agcaagggcg ccgagctgtt caccggcatc gtgcccatcc tgatcgagct gaatggcgat
     7981 gtgaatggcc acaagttcag cgtgagcggc gagggcgagg gcgatgccac ctacggcaag
     8041 ctgaccctga agttcatctg caccaccggc aagctgcctg tgccctggcc caccctggtg
     8101 accaccctga gctacggcgt gcagtgcttc tcacgctacc ccgatcacat gaagcagcac
     8161 gacttcttca agagcgccat gcctgagggc tacatccagg agcgcaccat cttcttcgag
     8221 gatgacggca actacaagtc gcgcgccgag gtgaagttcg agggcgatac cctggtgaat
     8281 cgcatcgagc tgaccggcac cgatttcaag gaggatggca acatcctggg caataagatg
     8341 gagtacaact acaacgccca caatgtgtac atcatgaccg acaaggccaa gaatggcatc
     8401 aaggtgaact tcaagatccg ccacaacatc gaggatggca gcgtgcagct ggccgaccac
     8461 taccagcaga atacccccat cggcgatggc cctgtgctgc tgcccgataa ccactacctg
     8521 tccacccaga gcgccctgtc caaggacccc aacgagaagc gcgatcacat gatctacttc
     8581 ggcttcgtga ccgccgccgc catcacccac ggcatggatg agctgtacaa gtgagcggcc
     8641 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     8701 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     8761 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8821 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     8881 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     8941 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     9001 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     9061 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     9121 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     9181 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     9241 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     9301 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     9361 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9421 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     9481 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     9541 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     9661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9721 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9781 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     9841 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     9901 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     9961 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
    10021 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
    10081 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
    10141 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
    10201 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
    10261 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
    10321 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
    10381 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
    10441 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
    10501 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
    10561 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
    10621 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
    10681 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
    10741 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    10801 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
    10861 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
    10921 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
    10981 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
    11041 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
    11101 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    11161 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    11221 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    11281 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11341 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    11401 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    11461 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    11521 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    11581 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    11641 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    11701 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    11761 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    11821 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    11881 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    11941 tgtggataac cgtattaccg ccatgcat
//
