LOCUS       sequence-437458-       11674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaIDR4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11674)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdIDR4
FEATURES             Location/Qualifiers
     source          1..11674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5155..5164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7621..8340
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    8462..8583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8499..8518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8553..8572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8590..9045)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8727..8748)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8939..8958
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9072..9176
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9178..9535
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9178..9369
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9201..9221)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9386..9521
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9570..10364
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9570..10364
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9624..9643)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10234..10253
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10552..10571)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10596..10643
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10972..11560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11461..11480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgaaacc aacaaaccat cctggggtct ccagctagtg gaattcaaaa cacaattggt
     2041 tctgttggca cagggcaaca gaatgccact tctttaagta acccaaatcc catagacccc
     2101 agctccatgc agcgagccta tgctgctctc ggactcccct acatgaacca gccccagacg
     2161 cagctgcagc ctcaggttcc tggccagcaa ccagcacagc ctcaaaccca ccagcagatg
     2221 aggactctca accccctggg aaataatcca atgaacattc cagcaggagg aataacaaca
     2281 gatcagcagc ccccaaactt gatttcagaa tcagctcttc cgacttccct gggggccaca
     2341 aacccactga tgaacgatgg ctccaactct ggtaacattg gaaccctcag cactatacca
     2401 acagcagctc ctccttctag caccggtgta aggaaaggct ggcacgaaca tgtcactcag
     2461 gacctgcgga gccatctagt gcataaactc gtccaagcca tcttcccaac acctgatccc
     2521 gcagctctaa aggatcgccg catggaaaac ctggtagcct atgctaagaa agtggaaggg
     2581 gacatgtacg agtctgccaa cagcagggat gaatattatc acttattagc agagaaaatc
     2641 tacaagatac ccgctcagag ccagtttctg ccacagaacc agttcccgtc atccagcggg
     2701 gcgatgagtg tgggcatggg gcagccgcca gcccaaacag gcgtgtcaca gggacaggtg
     2761 cctggtgctg ctcttcctaa ccctctcaac atgctggggc ctcaggccag ccagctacct
     2821 tgccctccag tgacacagtc accactgcac ccaacaccgc ctcctgcttc cacggctgct
     2881 ggcatgccat ctctccagca cacgacacca cctgggatga ctcctcccca gccagcagct
     2941 cccactcagc catcaactcc tgtgtcgtct tccgggcaga ctcccacccc gactcctggc
     3001 tcagtgccca gtgctaccca aacccagagc acccctacag tccaggcagc agcccaggcc
     3061 caggtgaccc cgcagcctca aaccccagtt cagcccccgt ctgtggctac ccctcagtca
     3121 tcgcagcaac agccgacgcc tgtgcacgcc cagcctcctg gcacaccgct ttcccaggca
     3181 gcagccagca ttgataacag agtccctacc ccctcctcgg tggccagcgc agaaaccaat
     3241 tcccagcagc caggacctga cgtacctgtg ctggaaatga agacggagac ccaagcagag
     3301 gacactgagc ccgatcctgg tgaatccaaa ggggagccca ggtctgagat gatggaggag
     3361 gatttgcaag gagcttccca agttaaagaa gaaacagaca tagcagagca gaaatcagaa
     3421 ccaatggaag tggatgaaaa gaaacctgaa gtgaaagtag aagttaaaga ggaagaagag
     3481 agtagcagta acggcacagc ctctcagtca acatctcctt cgcagccgcg caaaaaaatc
     3541 tttaaaccag aggagttacg ccaggccctc atgccaaccc tagaagcact gtatcgacag
     3601 gacccagagt cattaccttt ccggcagcct gtagatcccc agctcctcgg aattccagac
     3661 tattttgaca tcgtaaagaa tcccatggac ctctccacca tcaagcggaa gctggacaca
     3721 gggcaatacc aagagccctg gcagtacgtg gacgacgtct ggctcatgtt caacaatgcc
     3781 tggctctata atcgcaagac atcccgagtc tataagtttt gcagtaagct tgcagaggtc
     3841 tttgagcagg aaattgaccc tgtcatgcag tcccttggat attgctgtgg acgcaagtat
     3901 gagttttccc cacagacttt gtgctgctat gggaagcagc tgtgtaccat tcctcgcgat
     3961 gctgcctact acagctatca gaataggtat catttctgtg agaagtgttt cacagagatc
     4021 cagggcgaga atgtgaccct gggtgacgac ccttcacagc cccagacgac aatttcaaag
     4081 gatcagtttg aaaagaagaa aaatgatacc ttagaccccg aacctttcgt tgattgcaag
     4141 gagtgtggcc ggaagatgca tcagatttgc gttctgcact atgacatcat ttggccttca
     4201 ggttttgtgt gcgacaactg cttgaagaaa actggcagac ctcgaaaaga aaacaaattc
     4261 agtgctaaga ggctgcagac cacaagactg ggaaaccact tggaagaccg agtgaacaaa
     4321 tttttgcggc gccagaatca ccctgaagcc ggggaggttt ttgtccgagt ggtggccagc
     4381 tcagacaaga cggtggaggt caagcccggg atgaagtcac ggtttgtgga ttctggggaa
     4441 atgtctgaat ctttcccata tcgaaccaaa gctctgtttg cttttgagga aattgacggc
     4501 gtggatgtct gcttttttgg aatgcacgtc caagaatacg gctctgattg cccccctcca
     4561 aacacgaggc gtgtgtacat ttcttatctg gatagtattc atttcttccg gccacgttgc
     4621 ctccgcacag ccgtttacca tgagatcctt attggatatt tagagtatgt gaagaaatta
     4681 gggtatgtga cagggcacat ctgggcctgt cctccaagtg aaggagatga ttacatcttc
     4741 cattgccacc cacctgatca aaaaataccc aagccaaaac gactgcagga gtggtacaaa
     4801 aagatgctgg acaaggcgtt tgcagagcgg atcatccatg actacaagga tattttcaaa
     4861 caagcaactg aagacaggct caccagtgcc aaggaactgc cctattttga aggtgatttc
     4921 tggcccaatg tgttagaaga gagcattaag gaactagaac aagaagaaga ggagaggaaa
     4981 aaggaagaga gcactgcagc cagtgaaacc actgagggca gtcagggcga cagcaagaat
     5041 gccaagaaga agaacaacaa gaaaaccaac aagaacaaaa gcagcatcag ccgcgccaac
     5101 aagaagaagc ccagcatgcc caacgtgtcc aatgacctgt cccagaagct gtatgccacc
     5161 atggagaagc acaaggaggt cttcttcgtg atccacctgc acgctgggcc tgtcatcaac
     5221 accctgcccc ccatcgtcga ccccgacccc ctgctcagct gtgacctcat ggatgggcgc
     5281 gacgccttcc tcaccctcgc cagagacaag cactgggagt tctcctcctt gcgccgctcc
     5341 aagtggtcca cgctctgcat gctggtggag ctgcacaccc agggccagga ccgctttgtc
     5401 tacacctgca acgagtgcaa gcaccacgtg gagacgcgct ggcactgcac tgtgtgcgag
     5461 gactacgacc tctgcatcaa ctgctataac acgaagagcc atgcccataa gatggtgaag
     5521 tgggggctgg gcctggatga cgagggcagc agccagggcg agccacagtc aaagagcccc
     5581 caggagtcac gccggctgag catccagcgc tgcatccagt cgctggtgca cgcgtgccag
     5641 tgccgcaacg ccaactgctc gctgccatcc tgccagaaga tgaagcgggt ggtgcagcac
     5701 accaagggct gcaaacgcaa gaccaacggg ggctgcccgg tgtgcaagca gctcatcgcc
     5761 ctctgctgct accacgccaa gcactgccaa gaaaacaaat gccccgtgcc cttctgcctc
     5821 aacatcaaac acaagctccg ccagcagcag atccagcacc gcctgcagca ggcccagctc
     5881 atgcgccggc ggatggccac catgaacacc cgcaacgtgc ctcagcagag tctgccttct
     5941 cctacctcag caccgcccgg gacccccaca cagcagccca gcacacccca gacgccgcag
     6001 ccccctgccc agccccaacc ctcacccgtg agcatgtcac cagctggctt ccccagcgtg
     6061 gcccggactc agccccccac cacggtgtcc acagggaagc ctaccagcca ggtgccggcc
     6121 cccccacccc cggcccagcc ccctcctgca gcggtggaag cggctcggca gatcgagcgt
     6181 gaggcccagc agcagcagca cctgtaccgg gtgaacatca acaacagcat gcccccagga
     6241 cgcacgggca tggggacccc ggggagccag atggcccccg tgagcctgaa tgtgccccga
     6301 cccaaccagg tgagcgggcc cgtcatgccc agcatgcctc ccgggcagtg gcagcaggcg
     6361 ccccttcccc agcagcagcc catgccaggc ttgcccaggc ctgtgatatc catgcaggcc
     6421 caggcggccg tggctgggcc ccggatgccc agcgtgcagc cacccaggag catctcaccc
     6481 agcgctctgc aagacctgct gcggaccctg aagtcgccca gctcccctca gcagcaacag
     6541 caggtgctga acattctcaa atcaaacccg cagctaatgg cagctttcat caaacagcgc
     6601 acagccaagt acgtggccaa tcagcccggc atgcagcccc agcctggcct ccagtcccag
     6661 cccggcatgc aaccccagcc tggcatgcac cagcagccca gcctgcagaa cctgaatgcc
     6721 atgcaggctg gcgtgccgcg gcccggtgtg cctccacagc agcaggcgat gggaggcctg
     6781 aacccccagg gccaggcctt gaacatcatg aacccaggac acaaccccaa catggcgagt
     6841 atgaatccac agtaccgaga aatgttacgg aggcagctgc tgcagcagca gcagcaacag
     6901 cagcagcaac aacagcagca acagcagcag cagcaaggga gtgccggcat ggctgggggc
     6961 atggcggggc acggccagtt ccagcagcct caaggacccg gaggctaccc accggccatg
     7021 cagcagcagc agcgcatgca gcagcatctc cccctccagg gcagctccat gggccagatg
     7081 gcggctcaga tgggacagct tggccagatg gggcagccgg ggctgggggc agacagcacc
     7141 cccaacatcc agcaagccct gcagcagcgg attctgcagc aacagcagat gaagcagcag
     7201 attgggtccc caggccagcc gaaccccatg agcccccagc aacacatgct ctcaggacag
     7261 ccacaggcct cgcatctccc tggccagcag atcgccacgt cccttagtaa ccaggtgcgg
     7321 tctccagccc ctgtccagtc tccacggccc cagtcccagc ctccacattc cagcccgtca
     7381 ccacggatac agccccagcc ttcgccacac cacgtctcac cccagactgg ttccccccac
     7441 cccggactcg cagtcaccat ggccagctcc atagatcagg gacacttggg gaaccccgaa
     7501 cagagtgcaa tgctccccca gctgaacacc cccagcagga gtgcgctgtc cagcgaactg
     7561 tccctggtcg gggacaccac gggggacacg ctagagaagt ttgtggaggg cttgccggtc
     7621 atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat
     7681 ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga tgccacctac
     7741 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ctggcccacc
     7801 ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga tcacatgaag
     7861 cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg caccatcttc
     7921 ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg cgataccctg
     7981 gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat cctgggcaat
     8041 aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa ggccaagaat
     8101 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt gcagctggcc
     8161 gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc cgataaccac
     8221 tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga tcacatgatc
     8281 tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct gtacaagtga
     8341 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     8401 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     8461 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8581 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     8641 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     8701 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     8761 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     8821 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     8881 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     8941 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     9001 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     9061 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9121 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     9181 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9301 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9361 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9421 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9481 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     9541 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     9601 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     9661 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     9721 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     9781 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     9841 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     9901 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     9961 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
    10021 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
    10081 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
    10141 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
    10201 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
    10261 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
    10321 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
    10381 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
    10441 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    10501 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
    10561 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
    10621 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
    10681 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
    10741 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
    10801 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
    10861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    11041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    11101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    11161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    11221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    11281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    11341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    11401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    11461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    11521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    11581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    11641 tgattctgtg gataaccgta ttaccgccat gcat
//
