LOCUS       sequence-437461-       10399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaCFID in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10399)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdCFID
FEATURES             Location/Qualifiers
     source          1..10399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5533..5542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6346..7065
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    7187..7308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7224..7243)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7278..7297
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7315..7770)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7452..7473)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7664..7683
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7797..7901
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7903..8260
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7903..8094
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7926..7946)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8111..8246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8295..9089
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8295..9089
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8349..8368)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8959..8978
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9277..9296)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9321..9368
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9697..10285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10186..10205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgaaacc aacaaaccat cctggggtct ccagctagtg gaattcaaaa cacaattggt
     2041 tctgttggca cagggcaaca gaatgccact tctttaagta acccaaatcc catagacccc
     2101 agctccatgc agcgagccta tgctgctctc ggactcccct acatgaacca gccccagacg
     2161 cagctgcagc ctcaggttcc tggccagcaa ccagcacagc ctcaaaccca ccagcagatg
     2221 aggactctca accccctggg aaataatcca atgaacattc cagcaggagg aataacaaca
     2281 gatcagcagc ccccaaactt gatttcagaa tcagctcttc cgacttccct gggggccaca
     2341 aacccactga tgaacgatgg ctccaactct ggtaacattg gaaccctcag cactatacca
     2401 acagcagctc ctccttctag caccggtgta aggaaaggct ggcacgaaca tgtcactcag
     2461 gacctgcgga gccatctagt gcataaactc gtccaagcca tcttcccaac acctgatccc
     2521 gcagctctaa aggatcgccg catggaaaac ctggtagcct atgctaagaa agtggaaggg
     2581 gacatgtacg agtctgccaa cagcagggat gaatattatc acttattagc agagaaaatc
     2641 tacaagatac aaaaagaact agaagaaaaa cggaggtcgc gtttacataa acaaggcatc
     2701 ttggggaacc agccagcctt accagccccg ggggctcagc cccctgtgat tccacaggca
     2761 caacctgtga gacctccaaa tggacccctg tccctgccag tgaatcgcat gcaagtttct
     2821 caagggatga attcatttaa ccccatgtcc ttggggaacg tccagttgcc acaagcaccc
     2881 atgggacctc gtgcagcctc cccaatgaac cactctgtcc agatgaacag catgggctca
     2941 gtgccaggga tggccatttc tccttcccga atgcctcagc ctccgaacat gatgggtgca
     3001 cacaccaaca acatgatggc ccaggcgccc gctcagagcc agtttctgcc acagaaccag
     3061 ttcccgtcat ccagcggggc gatgagtgtg ggcatggggc agccgccagc ccaaacaggc
     3121 gtgtcacagg gacaggtgcc tggtgctgct cttcctaacc ctctcaacat gctggggcct
     3181 caggccagcc agctaccttg ccctccagtg acacagtcac cactgcaccc aacaccgcct
     3241 cctgcttcca cggctgctgg catgccatct ctccagcaca cgacaccacc tgggatgact
     3301 cctccccagc cagcagctcc cactcagcca tcaactcctg tgtcgtcttc cgggcagact
     3361 cccaccccga ctcctggctc agtgcccagt gctacccaaa cccagagcac ccctacagtc
     3421 caggcagcag cccaggccca ggtgaccccg cagcctcaaa ccccagttca gcccccgtct
     3481 gtggctaccc ctcagtcatc gcagcaacag ccgacgcctg tgcacgccca gcctcctggc
     3541 acaccgcttt cccaggcagc agccagcatt gataacagag tccctacccc ctcctcggtg
     3601 gccagcgcag aaaccaattc ccagcagcca ggacctgacg tacctgtgct ggaaatgaag
     3661 acggagaccc aagcagagga cactgagccc gatcctggtg aatccaaagg ggagcccagg
     3721 tctgagatga tggaggagga tttgcaagga gcttcccaag ttaaagaaga aacagacata
     3781 gcagagcaga aatcagaacc aatggaagtg gatgaaaaga aacctgaagt gaaagtagaa
     3841 gttaaagagg aagaagagag tagcagtaac ggcacagcct ctcagtcaac atctccttcg
     3901 cagccgcgca aaaaaatctt taaaccagag gagttacgcc aggccctcat gccaacccta
     3961 gaagcactgt atcgacagga cccagagtca ttacctttcc ggcagcctgt agatccccag
     4021 ctcctcggaa ttccagacta ttttgacatc gtaaagaatc ccatggacct ctccaccatc
     4081 aagcggaagc tggacacagg gcaataccaa gagccctggc agtacgtgga cgacgtctgg
     4141 ctcatgttca acaatgcctg gctctataat cgcaagacat cccgagtcta taagttttgc
     4201 agtaagcttg cagaggtctt tgagcaggaa attgaccctg tcatgcagtc ccttggatat
     4261 tgctgtggac gcaagtatga gttttcccca cagactttgt gctgctatgg gaagcagctg
     4321 tgtaccattc ctcgcgatgc tgcctactac agctatcaga ataggtatca tttctgtgag
     4381 aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc ttcacagccc
     4441 cagacgacaa tttcaaagga tcagtttgaa aagaagaaaa atgatacctt agaccccgaa
     4501 cctttcgttg attgcaagga gtgtggccgg aagatgcatc agatttgcgt tctgcactat
     4561 gacatcattt ggccttcagg ttttgtgtgc gacaactgct tgaagaaaac tggcagacct
     4621 cgaaaagaaa acaaattcag tgctaagagg ctgcagacca caagactggg aaaccacttg
     4681 gaagaccgag tgaacaaatt tttgcggcgc cagaatcacc ctgaagccgg ggaggttttt
     4741 gtccgagtgg tggccagctc agacaagacg gtggaggtca agcccgggat gaagtcacgg
     4801 tttgtggatt ctggggaaat gtctgaatct ttcccatatc gaaccaaagc tctgtttgct
     4861 tttgaggaaa ttgacggcgt ggatgtctgc ttttttggaa tgcacgtcca agaatacggc
     4921 tctgattgcc cccctccaaa cacgaggcgt gtgtacattt cttatctgga tagtattcat
     4981 ttcttccggc cacgttgcct ccgcacagcc gtttaccatg agatccttat tggatattta
     5041 gagtatgtga agaaattagg gtatgtgaca gggcacatct gggcctgtcc tccaagtgaa
     5101 ggagatgatt acatcttcca ttgccaccca cctgatcaaa aaatacccaa gccaaaacga
     5161 ctgcaggagt ggtacaaaaa gatgctggac aaggcgtttg cagagcggat catccatgac
     5221 tacaaggata ttttcaaaca agcaactgaa gacaggctca ccagtgccaa ggaactgccc
     5281 tattttgaag gtgatttctg gcccaatgtg ttagaagaga gcattaagga actagaacaa
     5341 gaagaagagg agaggaaaaa ggaagagagc actgcagcca gtgaaaccac tgagggcagt
     5401 cagggcgaca gcaagaatgc caagaagaag aacaacaaga aaaccaacaa gaacaaaagc
     5461 agcatcagcc gcgccaacaa gaagaagccc agcatgccca acgtgtccaa tgacctgtcc
     5521 cagaagctgt atgccaccat ggagaagcac aaggaggtct tcttcgtgat ccacctgcac
     5581 gctgggcctg tcatcaacac cctgcccccc atcgtcgacc ccgaccccct gctcagctgt
     5641 gacctcatgg atgggcgcga cgccttcctc accctcgcca gagacaagca ctgggagttc
     5701 tcctccttgc gccgctccaa gtggtccacg ctctgcatgc tggtggagct gcacacccag
     5761 ggccaggacc gctttgtcta cacctgcaac gagtgcaagc accacgtgga gacgcgctgg
     5821 cactgcactg tgtgcgagga ctacgacctc tgcatcaact gctataacac gaagagccat
     5881 gcccataaga tggtgaagtg ggggctgggc ctggatgacg agggcagcag ccagggcgag
     5941 ccacagtcaa agagccccca ggagtcacgc cggctgagca tccagcgctg catccagtcg
     6001 ctggtgcacg cgtgccagtg ccgcaacgcc aactgctcgc tgccatcctg ccagaagatg
     6061 aagcgggtgg tgcagcacac caagggctgc aaacgcaaga ccaacggggg ctgcccggtg
     6121 tgcaagcagc tcatcgccct ctgctgctac cacgccaagc actgccaaga aaacaaatgc
     6181 cccgtgccct tctgcctcaa catcaaacac aagctccgcc agcagcagat ccagcaccgc
     6241 ctgcagcagg cccagctcat gcgccggcgg atggccacca tgaacacccg caacgtgcct
     6301 cagcagagtc tgccttctcc tacctcagca ccgcccgggc cggtcatggt gagcaagggc
     6361 gccgagctgt tcaccggcat cgtgcccatc ctgatcgagc tgaatggcga tgtgaatggc
     6421 cacaagttca gcgtgagcgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     6481 aagttcatct gcaccaccgg caagctgcct gtgccctggc ccaccctggt gaccaccctg
     6541 agctacggcg tgcagtgctt ctcacgctac cccgatcaca tgaagcagca cgacttcttc
     6601 aagagcgcca tgcctgaggg ctacatccag gagcgcacca tcttcttcga ggatgacggc
     6661 aactacaagt cgcgcgccga ggtgaagttc gagggcgata ccctggtgaa tcgcatcgag
     6721 ctgaccggca ccgatttcaa ggaggatggc aacatcctgg gcaataagat ggagtacaac
     6781 tacaacgccc acaatgtgta catcatgacc gacaaggcca agaatggcat caaggtgaac
     6841 ttcaagatcc gccacaacat cgaggatggc agcgtgcagc tggccgacca ctaccagcag
     6901 aataccccca tcggcgatgg ccctgtgctg ctgcccgata accactacct gtccacccag
     6961 agcgccctgt ccaaggaccc caacgagaag cgcgatcaca tgatctactt cggcttcgtg
     7021 accgccgccg ccatcaccca cggcatggat gagctgtaca agtgagcggc cgcgactcta
     7081 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     7141 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     7201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     7321 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     7381 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     7441 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     7501 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     7561 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     7621 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     7681 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     7741 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     7801 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7861 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     7921 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7981 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8041 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8101 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8161 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8221 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     8281 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     8341 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     8401 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     8461 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     8521 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     8581 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     8641 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     8701 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     8761 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     8821 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     8881 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     8941 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     9001 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     9061 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     9121 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     9181 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9241 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     9301 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     9361 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     9421 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     9481 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     9541 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9601 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9661 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9721 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9781 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9841 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9901 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9961 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10021 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10081 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10141 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10201 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10261 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10321 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10381 ccgtattacc gccatgcat
//
