LOCUS       sequence-437462-       11938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaAL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11938)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdAL
FEATURES             Location/Qualifiers
     source          1..11938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5419..5428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7885..8604
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    8726..8847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8763..8782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8817..8836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8854..9309)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8991..9012)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9203..9222
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9336..9440
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9442..9799
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9442..9633
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9465..9485)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9650..9785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9834..10628
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9834..10628
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9888..9907)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10498..10517
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10816..10835)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10860..10907
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11236..11824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11725..11744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgaaacc aacaaaccat cctggggtct ccagctagtg gaattcaaaa cacaattggt
     2041 tctgttggca cagggcaaca gaatgccact tctttaagta acccaaatcc catagacccc
     2101 agctccatgc agcgagccta tgctgctctc ggactcccct acatgaacca gccccagacg
     2161 cagctgcagc ctcaggttcc tggccagcaa ccagcacagc ctcaaaccca ccagcagatg
     2221 aggactctca accccctggg aaataatcca atgaacattc cagcaggagg aataacaaca
     2281 gatcagcagc ccccaaactt gatttcagaa tcagctcttc cgacttccct gggggccaca
     2341 aacccactga tgaacgatgg ctccaactct ggtaacattg gaaccctcag cactatacca
     2401 acagcagctc ctccttctag caccggtgta aggaaaggct ggcacgaaca tgtcactcag
     2461 gacctgcgga gccatctagt gcataaactc gtccaagcca tcttcccaac acctgatccc
     2521 gcagctctaa aggatcgccg catggaaaac ctggtagcct atgctaagaa agtggaaggg
     2581 gacatgtacg agtctgccaa cagcagggat gaatattatc acttattagc agagaaaatc
     2641 tacaagatac aaaaagaact agaagaaaaa cggaggtcgc gtttacataa acaaggcatc
     2701 ttggggaacc agccagcctt accagccccg ggggctcagc cccctgtgat tccacaggca
     2761 caacctgtga gacctccaaa tggacccctg tccctgccag tgaatcgcat gcaagtttct
     2821 caagggatga attcatttaa ccccatgtcc ttggggaacg tccagttgcc acaagcaccc
     2881 atgggacctc gtgcagcctc cccaatgaac cactctgtcc agatgaacag catgggctca
     2941 gtgccaggga tggccatttc tccttcccga atgcctcagc ctccgaacat gatgggtgca
     3001 cacaccaaca acatgatggc ccaggcgccc gctcagagcc agtttctgcc acagaaccag
     3061 ttcccgtcat ccagcggggc gatgagtgtg ggcatggggc agccgccagc ccaaacaggc
     3121 gtgtcacagg gacaggtgcc tggtgctgct cttcctaacc ctctcaacat gctggggcct
     3181 caggccagcc agctaccttg ccctccagtg acacagtcac cactgcaccc aacaccgcct
     3241 cctgcttcca cggctgctgg catgccatct ctccagcaca cgacaccacc tgggatgact
     3301 cctccccagc cagcagctcc cactcagcca tcaactcctg tgtcgtcttc cgggcagact
     3361 cccaccccga ctcctggctc agtgcccagt gctacccaaa cccagagcac ccctacagtc
     3421 caggcagcag cccaggccca ggtgaccccg cagcctcaaa ccccagttca gcccccgtct
     3481 gtggctaccc ctcagtcatc gcagcaacag ccgacgcctg tgcacgccca gcctcctggc
     3541 acaccgcttt cccaggcagc agccagcatt gataacagag tccctacccc ctcctcggtg
     3601 gccagcgcag aaaccaattc ccagcagcca ggacctgacg tacctgtgct ggaaatgaag
     3661 acggagaccc aagcagagga cactgagccc gatcctggtg aatccaaagg ggagcccagg
     3721 tctgagatga tggaggagga tttgcaagga gcttcccaag ttaaagaaga aacagacata
     3781 gcagagcaga aatcagaacc aatggaagtg gatgaaaaga aacctgaagt gaaagtagaa
     3841 gttaaagagg aagaagagag tagcagtaac ggcacagcct ctcagtcaac atctccttcg
     3901 cagccgcgca aaaaaatctt taaaccagag gagttacgcc aggccctcat gccaacccta
     3961 gaagcactgt atcgacagga cccagagtca ttacctttcc ggcagcctgt agatccccag
     4021 ctcctcggaa ttccagacta ttttgacatc gtaaagaatc ccatggacct ctccaccatc
     4081 aagcggaagc tggacacagg gcaataccaa gagccctggc agtacgtgga cgacgtctgg
     4141 ctcatgttca acaatgcctg gctctataat cgcaagacat cccgagtcta taagttttgc
     4201 agtaagcttg cagaggtctt tgagcaggaa attgaccctg tcatgcagtc ccttggatat
     4261 tgctgtggac gcaagtatga gttttcccca cagactttgt gctgctatgg gaagcagctg
     4321 tgtaccattc ctcgcgatgc tgcctactac agctatcaga ataggtatca tttctgtgag
     4381 aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc ttcacagccc
     4441 cagacgacaa tttcaaagga tcagtttgaa aagaagaaaa atgatacctt agaccccgaa
     4501 cctttcgttg attgcaagga gtgtggccgg aagatgcatc agatttgcgt tctgcactat
     4561 gacatcattt ggccttcagg ttttgtgtgc gacaactgct tgaagaaaac tggcagacct
     4621 cgaaaagaaa acaaattcag tgctaagagg ctgcagacca caagactggg aaaccacttg
     4681 gaagaccgag tgaacaaatt tttgcggcgc cagaatcacc ctgaagccgg ggaggttttt
     4741 gtccgagtgg tggccagctc agacaagacg gtggaggtca agcccgggat gaagtcacgg
     4801 tttgtggatt ctggggaaat gtctgaatct ttcccatatc gaaccaaagc tctgtttgct
     4861 tttgaggaaa ttgacggcgt ggatgtctgc ttttttggaa tgcacgtcca agaatacggc
     4921 tctgattgcc cccctccaaa cacgaggcgt gtgtacattt cttatctgga tagtattcat
     4981 ttcttccggc cacgttgcct ccgcacagcc gtttaccatg agatccttat tggatattta
     5041 gagtatgtga agaaattagg gtatgtgaca gggcacatct gggcctgtcc tccaagtgaa
     5101 ggagatgatt acatcttcca ttgccaccca cctgatcaaa aaatacccaa gccaaaacga
     5161 ctgcaggagt ggtacaaaaa gatgctggac aaggcgtttg cagagcggat catccatgac
     5221 tacaaggata ttttcaaaca agcaactgaa gacaggctca ccagtgccaa ggaactgccc
     5281 tattttgaag gtgatttctg gcccaatgtg ttagaagaga gcattaagga actagaaggc
     5341 agtgccggga gcgccgcagg gagtggtgaa tttcccagca tgcccaacgt gtccaatgac
     5401 ctgtcccaga agctgtatgc caccatggag aagcacaagg aggtcttctt cgtgatccac
     5461 ctgcacgctg ggcctgtcat caacaccctg ccccccatcg tcgaccccga ccccctgctc
     5521 agctgtgacc tcatggatgg gcgcgacgcc ttcctcaccc tcgccagaga caagcactgg
     5581 gagttctcct ccttgcgccg ctccaagtgg tccacgctct gcatgctggt ggagctgcac
     5641 acccagggcc aggaccgctt tgtctacacc tgcaacgagt gcaagcacca cgtggagacg
     5701 cgctggcact gcactgtgtg cgaggactac gacctctgca tcaactgcta taacacgaag
     5761 agccatgccc ataagatggt gaagtggggg ctgggcctgg atgacgaggg cagcagccag
     5821 ggcgagccac agtcaaagag cccccaggag tcacgccggc tgagcatcca gcgctgcatc
     5881 cagtcgctgg tgcacgcgtg ccagtgccgc aacgccaact gctcgctgcc atcctgccag
     5941 aagatgaagc gggtggtgca gcacaccaag ggctgcaaac gcaagaccaa cgggggctgc
     6001 ccggtgtgca agcagctcat cgccctctgc tgctaccacg ccaagcactg ccaagaaaac
     6061 aaatgccccg tgcccttctg cctcaacatc aaacacaagc tccgccagca gcagatccag
     6121 caccgcctgc agcaggccca gctcatgcgc cggcggatgg ccaccatgaa cacccgcaac
     6181 gtgcctcagc agagtctgcc ttctcctacc tcagcaccgc ccgggacccc cacacagcag
     6241 cccagcacac cccagacgcc gcagccccct gcccagcccc aaccctcacc cgtgagcatg
     6301 tcaccagctg gcttccccag cgtggcccgg actcagcccc ccaccacggt gtccacaggg
     6361 aagcctacca gccaggtgcc ggccccccca cccccggccc agccccctcc tgcagcggtg
     6421 gaagcggctc ggcagatcga gcgtgaggcc cagcagcagc agcacctgta ccgggtgaac
     6481 atcaacaaca gcatgccccc aggacgcacg ggcatgggga ccccggggag ccagatggcc
     6541 cccgtgagcc tgaatgtgcc ccgacccaac caggtgagcg ggcccgtcat gcccagcatg
     6601 cctcccgggc agtggcagca ggcgcccctt ccccagcagc agcccatgcc aggcttgccc
     6661 aggcctgtga tatccatgca ggcccaggcg gccgtggctg ggccccggat gcccagcgtg
     6721 cagccaccca ggagcatctc acccagcgct ctgcaagacc tgctgcggac cctgaagtcg
     6781 cccagctccc ctcagcagca acagcaggtg ctgaacattc tcaaatcaaa cccgcagcta
     6841 atggcagctt tcatcaaaca gcgcacagcc aagtacgtgg ccaatcagcc cggcatgcag
     6901 ccccagcctg gcctccagtc ccagcccggc atgcaacccc agcctggcat gcaccagcag
     6961 cccagcctgc agaacctgaa tgccatgcag gctggcgtgc cgcggcccgg tgtgcctcca
     7021 cagcagcagg cgatgggagg cctgaacccc cagggccagg ccttgaacat catgaaccca
     7081 ggacacaacc ccaacatggc gagtatgaat ccacagtacc gagaaatgtt acggaggcag
     7141 ctgctgcagc agcagcagca acagcagcag caacaacagc agcaacagca gcagcagcaa
     7201 gggagtgccg gcatggctgg gggcatggcg gggcacggcc agttccagca gcctcaagga
     7261 cccggaggct acccaccggc catgcagcag cagcagcgca tgcagcagca tctccccctc
     7321 cagggcagct ccatgggcca gatggcggct cagatgggac agcttggcca gatggggcag
     7381 ccggggctgg gggcagacag cacccccaac atccagcaag ccctgcagca gcggattctg
     7441 cagcaacagc agatgaagca gcagattggg tccccaggcc agccgaaccc catgagcccc
     7501 cagcaacaca tgctctcagg acagccacag gcctcgcatc tccctggcca gcagatcgcc
     7561 acgtccctta gtaaccaggt gcggtctcca gcccctgtcc agtctccacg gccccagtcc
     7621 cagcctccac attccagccc gtcaccacgg atacagcccc agccttcgcc acaccacgtc
     7681 tcaccccaga ctggttcccc ccaccccgga ctcgcagtca ccatggccag ctccatagat
     7741 cagggacact tggggaaccc cgaacagagt gcaatgctcc cccagctgaa cacccccagc
     7801 aggagtgcgc tgtccagcga actgtccctg gtcggggaca ccacggggga cacgctagag
     7861 aagtttgtgg agggcttgcc ggtcatggtg agcaagggcg ccgagctgtt caccggcatc
     7921 gtgcccatcc tgatcgagct gaatggcgat gtgaatggcc acaagttcag cgtgagcggc
     7981 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     8041 aagctgcctg tgccctggcc caccctggtg accaccctga gctacggcgt gcagtgcttc
     8101 tcacgctacc ccgatcacat gaagcagcac gacttcttca agagcgccat gcctgagggc
     8161 tacatccagg agcgcaccat cttcttcgag gatgacggca actacaagtc gcgcgccgag
     8221 gtgaagttcg agggcgatac cctggtgaat cgcatcgagc tgaccggcac cgatttcaag
     8281 gaggatggca acatcctggg caataagatg gagtacaact acaacgccca caatgtgtac
     8341 atcatgaccg acaaggccaa gaatggcatc aaggtgaact tcaagatccg ccacaacatc
     8401 gaggatggca gcgtgcagct ggccgaccac taccagcaga atacccccat cggcgatggc
     8461 cctgtgctgc tgcccgataa ccactacctg tccacccaga gcgccctgtc caaggacccc
     8521 aacgagaagc gcgatcacat gatctacttc ggcttcgtga ccgccgccgc catcacccac
     8581 ggcatggatg agctgtacaa gtgagcggcc gcgactctag atcataatca gccataccac
     8641 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     8701 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     8761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8821 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     8881 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     8941 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     9001 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     9061 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     9121 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     9181 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     9241 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     9301 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9361 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9421 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     9481 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9541 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9601 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9661 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9721 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9781 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     9841 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     9901 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     9961 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
    10021 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
    10081 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
    10141 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
    10201 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
    10261 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
    10321 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
    10381 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
    10441 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
    10501 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
    10561 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
    10621 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
    10681 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    10741 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    10801 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
    10861 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
    10921 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
    10981 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
    11041 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
    11101 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    11161 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11221 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11341 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    11401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    11521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    11581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    11641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    11701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    11761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    11821 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    11881 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
