LOCUS       sequence-43749-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: pBAD-PSmOrange2
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     521..540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttg ggctaacagg
       61 aggaattaac catggggagc caccatcacc atcaccatgg cagatctatg gtgagcaagg
      121 gcgaggagaa taatatggcc atcattaagg agttcatgcg cttcaaggtg cacatggagg
      181 gcactgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccac ccctacgagg
      241 gctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      301 tcctgtcccc tctcatcacc tacggctcca aggcgtacgt gaagcacccc gccgacatcc
      361 ccgactactt caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg
      421 aggacggcgg cgtggtgacc gtgacccagg actcctctct gcaggacggc gagttcatct
      481 acaaggtgaa gatgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      541 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      601 agatcaggat gaggctgaag ctgaaggatg gcggccacta cacctccgag gtcaagacta
      661 cctacaaggc caagaagtcc gtgctgctgc ccggcgccta catcgtcggc atcaagttgg
      721 acattacctc acacaacgag gactacacca tcgtggaaca gtatgaacgc agcgaggccc
      781 gccact
//
