LOCUS       sequence-437491-        3027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS of CPH from N. nambi codon optimized for N.benthamiana. This 
            gene is the final step and recycling of the bioluminiscente pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB3474
FEATURES             Location/Qualifiers
     source          1..3027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             76..84
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      984..1041
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1065..1082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1236..1824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1316..1335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2007..2101)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2122..2781)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2696..2715
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2943..2961
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2965..3008)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(3011..3027,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg aatggcccca ataagttcca cgtggtctag gctcataagg
       61 tttgtggctg tggaaacctc cttagttcac atcggcgagc ctattgatgc aacaatggac
      121 gtgggcttag cacgtaggga agggaagaca atacaagcct atgagatcat cggcagtggc
      181 tctgctttag atctgtctgc ccaagtgtca aagaacgtac ttacggtccg tgagctctta
      241 atgcccttga gccgtgagga gattaaaacg gtaagatgcc ttggccttaa ttatcccgtc
      301 cacgccacag aagccaacgt ggccgtgccg aaattcccga acctttttta caaacccgta
      361 acctccctca tcggaccagg tgggttgata accatccctt cagttgtcca accacctaaa
      421 gagcaccaga gcgattacga agccgagttg gttatcgtca tcggtaaagc cgccaaaaat
      481 gtcagtgaag atgaagctct ggactacgtg ttgggctaca ccgcagccaa tgacatctca
      541 tttaggaaac atcagttggc cgtttctcaa tggtcattct ccaagggttt tgatggcacg
      601 aatcccctgg gaccgtgcct ggtttctgct tcctctattc cagatccaca ggatattccg
      661 atccaatgta aactcaacgg tggggtggtg caaaatggaa acactcgtga tcagattttt
      721 aatgtcaaga aaacaatctc ttttttaagc caagggacta cgttggagcc tggttccata
      781 atcctgacgg ggacgcccga cggagtgggc ttcgtaagaa acccaccgct ttatttgaag
      841 gacggtgatg aagtaatgac ttggatcgga tcaggtatag gaaccttagc caacactgtc
      901 cgagaggagc agacttgttt cgcttcagga gggcacgagt aagctttgag accactcatc
      961 gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
     1021 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
     1081 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     1261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1861 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1921 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1981 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     2041 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     2101 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt actgttgtaa
     2161 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     2221 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     2281 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     2341 ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     2401 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     2461 actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     2521 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     2581 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     2641 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     2701 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     2761 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     2821 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     2881 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2941 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     3001 atgatttctg gaattcgcgg ccgcttc
//
