LOCUS       sequence-43750-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1046
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(736..769)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctctctcggc agcttgaact tgtcgaacaa gagcttgcca gtccctcctc agggcgtgga
       61 cccggccaga ggtcagaggt tacacctccc gccctccgct cgacgttcgc ggggagggga
      121 gctgtggccg gcgctctgca attccgccag cccacccccc ggtccctctt tctccagact
      181 cggtgtcatc ccgggctttc aatcagagtt cgcctgtgcg tgcaagtgaa tgctggagtc
      241 ccccgagccc tgggagggcg cagcaggtcc tccgcctggg cgtcccaggg ggagcgggct
      301 gtgcgatcaa gcatggtgtc aagaagatgc ctacggaata cacacacacc ccattcagcc
      361 ctggcgaaag gcacaacact gcgcgcacac acccgcagca tatgcatcct gtgttctgtg
      421 cgcccggctg taattatcgg cgcgctttgc ataaatcaca atacagttct gaaatcatcg
      481 tgatgacttt ctggcatgac tggttcagtg gtaacggcgg caacaagctc cagggtaggc
      541 gggaggggct gaggccccgt cgcccaccag gtcccaaata agcctggagc cttagtgcgg
      601 gctgccttct actcaggcaa agctgctgca ggccacgggg gacctcggat ttaaggcgta
      661 gacgcgtggc cggcctagga gctcgaattc gaaccccttc gagcttggct ggacgtaaac
      721 tcctcttcag acctaataac ttcgtatagc atacattata cgaagttata tta
//
