LOCUS       sequence-43751-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: pPSmOrange2-N1
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    20..100
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     715..738
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa
       61 ttctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg
      121 gcgaggagaa taatatggcc atcattaagg agttcatgcg cttcaaggtg cacatggagg
      181 gcactgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccac ccctacgagg
      241 gctttcagac cgctaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
      301 tcctgtcccc tctcatcacc tacggctcca aggcctacgt gaagcacccc gccgacatcc
      361 ccgactactt caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg
      421 aggacggcgg cgtggtgacc gtgacccagg actcctctct gcaggacggc gagttcatct
      481 acaaggtgaa gatgcgcggc accaacttcc cctccgacgg ccccgtgatg cagaagaaga
      541 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      601 agatcaggat gaggctgaag ctgaaggatg gcggccacta cacctccgag gtcaagacta
      661 cctacaaggc caagaagtcc gtgctgctgc ccggcgccta catcgtcggc atcaagctgg
      721 acatcacctc ccacaacgag
//
