LOCUS       sequence-437529-        6866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression of DmPABPC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6866)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-6P-1-DmPABPC1_J
FEATURES             Location/Qualifiers
     source          1..6866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(179..184,185..200,201..207)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    215..231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             254..907
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     865..887
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             914..937
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(2912..2934)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3079..3097)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3165..3269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3270..3338,3339..4130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3488..3507)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4301..4889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4790..4809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5043..5060
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5133..5210
                     /gene="lacI"
                     /label=lacI promoter
     CDS             5211..6293
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5230..5249)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(6342..6347,6348..6365,6366..6372)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6380..6396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6404..6420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6404..6420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(6416..6866,1..77)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(6436..6453)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6436..6452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6445..6467)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tatcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat
       61 ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg
      121 gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt
      181 gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca
      241 ggaaacagta ttcatgtccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc
      301 cactcgactt cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga
      361 tgaaggtgat aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc
      421 ttattatatt gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc
      481 tgacaagcac aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga
      541 aggagcggtt ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga
      601 aactctcaaa gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg
      661 tttatgtcat aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta
      721 tgacgctctt gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt
      781 agtttgtttt aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag
      841 caagtatata gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc
      901 tccaaaatcg gatctggaag ttctgttcca ggggcccctg ggatccatgg cttctctata
      961 cgtcggtgat ctcccacagg acgtcaacga atcgggactt tttgacaagt tctcgtctgc
     1021 tagtccagtg ctgtccattc gtgtctgccg cgatgtgatt acccgtcgct cgttgggcta
     1081 tgcctacgtc aacttccagc agccagccga tgctgagcgt gctttggaca ccatgaactt
     1141 tgacctggtt cgcaacaagc ccattcgcat tatgtggtct cagcgtgatc cgtctcttcg
     1201 tcgctccggt gtgggcaacg tgttcatcaa gaacttagat agggccatcg acaacaaggc
     1261 aatctacgac actttctccg ccttcggcaa cattttaagc tgcaaggtag ccaccgatga
     1321 aaagggcaac tcaaagggct atggattcgt ccacttcgag accgaggagg ccgcaaacac
     1381 gtccatcgac aaagtcaatg gcatgttgct taacggcaag aaggtctacg tgggtaagtt
     1441 catcccgcgc aaggagcgcg agaaggagct gggcgagaag gctaagctct tcaccaatgt
     1501 atatgtgaag aacttcactg aggatttcga cgatgaaaaa ctgaaggaat tctttgagcc
     1561 ctacggcaag ataaccagct acaaggtcat gtccaaggag gatggcaaga gcaagggctt
     1621 cggattcgtt gcattcgaga caacagaggc tgctgaggcg gccgttcagg ctctcaatgg
     1681 caaggacatg ggagagggca agtccctgta cgtggctcgt gcccagaaga aggccgaacg
     1741 ccagcaggag ctgaagcgca agttcgagga actgaagcag aagcgtcacg agtcagtgtt
     1801 cggcgttaac ttgtacgtta agaacctgga cgacacaatc gatgacgatc gtctgcgcat
     1861 cgcgttctct ccatacggca acatcacatc ggccaaggtc atgaccgatg aggagggtcg
     1921 ctccaaggga ttcggcttcg tgtgcttcaa tgccgcaagc gaggctacct gcgccgtcac
     1981 cgagctgaac ggtcgcgtcg tcggcagtaa gccgttgtac gttgctttgg cccaaaggaa
     2041 ggaggagcgc aaggctcatc tcgcctcgca gtacatgcgt cacatgaccg gcatgcgtat
     2101 gcagcagctg ggacagatct accagcccaa cgcggctagc ggcttctttg tgccgaccct
     2161 tccgtcaaat cagcgtttct tcggttccca ggtggccact cagatgagaa acacaccccg
     2221 ctgggtgccc caggtgcgtc ccccagcagc catacagggt gtccaggccg gagctgccgc
     2281 tgctggtggc ttccagggca cagccggtgc ggtgcccact cagttccgtt cggctgctgc
     2341 tggcgctcgc ggggcacagc ctcaggtgca gggcacacac gccgccgctg ctgcggctaa
     2401 caacatgcgc aacactggag cacgcgctat tactggccag caaactgcag cacccaacat
     2461 gcagatcccc ggagcccaaa tcgctggcgg tgcccagcag cgtacttcca actataagta
     2521 cacctcaaac atgcgtaatc ctccagtgcc acagttgcat cagacccagc caattccaca
     2581 gcaattgcaa ggaaagaatt ctgagaagct cattgcctcg ttgctggcca acgccaagcc
     2641 gcaggagcag aaacagatcc tgggcgagcg tctgtatccg atgattgaac atatgcacgc
     2701 caatttggct ggtaagatca ccggcatgtt gttggagatc gaaaactctg agctcttgca
     2761 catgatcgag gatcaggagg cccttaaggc caaggtggag gaggctgtgg ccgtgcttca
     2821 ggtgcaccgc gtcaccgagc ccgccaacta agcggccgca tcgtgactga ctgacgatct
     2881 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     2941 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3001 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     3061 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3121 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3181 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3241 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3301 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3361 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3421 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3481 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     3541 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3601 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3661 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3721 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3781 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     3841 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3901 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3961 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4021 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4081 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4141 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4201 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4261 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4321 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4381 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4441 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4501 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4561 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4621 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4681 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4741 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4801 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4861 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4921 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4981 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5041 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     5101 atctgtgcgg tatttcacac cgcataaatt ccgacaccat cgaatggcgc aaaacctttc
     5161 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     5221 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     5281 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     5341 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     5401 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     5461 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     5521 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     5581 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     5641 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     5701 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     5761 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     5821 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     5881 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     5941 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     6001 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     6061 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     6121 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     6181 tgaaaagaaa aaccacgcta gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6241 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     6301 gcaattaatg taagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     6361 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     6421 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6481 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6541 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
     6601 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
     6661 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
     6721 caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
     6781 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
     6841 tatttttgat ggcgttggaa ttagct
//
