LOCUS       sequence-437531-       10424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF 
            that is fused with S-phase restricted (SPR) degron at its 
            C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Cho T, Hoeg L, Setiaputra D, Durocher D
  TITLE     NFATC2IP is a mediator of SUMO-dependent genome integrity.
  JOURNAL   Genes Dev. 2024 Apr 17;38(5-6):233-252. doi: 10.1101/gad.350914.123.
  PUBMED    38503515
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2024-04-17"; volume: "38(5-6):233-252. doi"; pages: " 
            10.1101/gad.350914.123"
COMMENT     Sequence Label: pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR
FEATURES             Location/Qualifiers
     source          1..10424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2624..3802
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2855..3793
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3750..3770
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3823..3832
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3829..3831,3832..3834,3835..4545)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3829..3831,3832..3834,3835..4545)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3874..3895)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4135..4154)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4482..4503
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5839..6168
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    join(6482..7056,7057..7059,7060..7068)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6649..6666)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6876..6895
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7648..7701
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(7904..7920)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7905..7921)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8430..8610
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8672..9260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8752..8771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9431..10222,10223..10291))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10054..10073
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10292..10396)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagcg tgaggctccg gtgcccgtca
     2641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2701 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2821 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2881 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2941 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3001 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3061 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3121 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3181 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3241 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3301 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3361 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3421 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3481 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3541 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3601 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3661 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3721 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3781 tcttccattt caggtgtcgt gagcggccgc tgaactgaat tcgccaccat ggtgagcaag
     3841 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3901 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3961 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4021 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     4081 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4141 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4201 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4261 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     4321 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     4381 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     4441 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     4501 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgc gccagcggag
     4561 cctgtgggga agcggggccg ctggtccgga ggtagcggtg ccggccgagg gggtcggggc
     4621 ggctggggcg gtcggggccg gcgtcctcgg gcccagcggt ctccatcccg gggcacgctg
     4681 gacgtagtgt ctgtggactt ggtcaccgac agcgatgagg aaattctgga ggtcgccacc
     4741 gctcgcggtg ccgcggacga ggttgaggtg gagcccccgg agcccccggg gccggtcgcg
     4801 tcccgggata acagcaacag tgacagcgaa ggggaggaca ggcggcccgc aggacccccg
     4861 cgggagccgg tcaggcggcg gcggcggctg gtgctggatc cgggggaggc gccgctggtt
     4921 ccggtgtact cggggaaggt taaaagcagc cttcgcctta tcccagatga tctatccctc
     4981 ctgaaactct accctccagg ggatgaggaa gaggcagagc tggcagattc gagtggtctc
     5041 taccatgagg gctccccatc accaggctct ccctggaaga caaagctgag gactaaggat
     5101 aaagaagaga agaaaaagac agagtttctg gatctggaca actctcctct gtccccacct
     5161 tcaccaagga ccaaaagcag aacgcatact cgggcactca agaagttaag tgaggtgaac
     5221 aagcgcctcc aggatctccg ttcctgtctg agccccaagc cacctcaggg tcaagagcaa
     5281 cagggccaag aggatgaagt ggtcttggtg gaagggccca ccctcccaga gaccccccga
     5341 ctcttcccac tcaaaatccg ttgccgggct gacctggtca gattgcccct caggatgtcg
     5401 gagcccctgc agagtgtggt ggaccacatg gccacccacc ttggggtgtc cccaagcagg
     5461 atccttttgc tttttggaga gacagagcta tcacctactg ccactcccag gaccctaaag
     5521 ctcggagtgg ctgacatcat tgactgtgtg gtactaacaa gttctccaga ggccacagag
     5581 acgtcccaac agctccagct ccgggtgcag ggaaaggaga aacaccagac actggaagtc
     5641 tcactgtctc gagattcccc tctaaagacc ctcatgtccc actatgagga ggccatggga
     5701 ctgtcgggac ggaagctctc cttcttcttt gatgggacaa agctttcagg cagggagctg
     5761 ccagctgacc tgggcatgga atctggggac ctcattgagg tctggggcgg atatccatca
     5821 cactggcggc cgctcgagat gaatcccagt atgaagcaga aacaagaaga aatcaaagag
     5881 aatataaaga atagttctgt cccaagaaga actctgaaga tgattcagcc ttctgcatct
     5941 ggatctcttg ttggaagaga aaatgagctg tccgcaggct tgtccaaaag gaaacatcgg
     6001 aatgaccact taacatctac aacttccagc cctggggtta ttgtcccaga atctagtgaa
     6061 aataaaaatc ttggaggagt cacccaggag tcatttgatc ttatgattaa agaaaatcca
     6121 tcctctcagt attggaagga agtggcagaa aaacggagaa aggcgctggg cgggggatcc
     6181 gccagcccgc cgtcccccgc gcgatggagc ctgggacgga agcgcagagc cgacggcagg
     6241 cgctggaggc ccgaagacgc cgaggaggca gagcaccgcg gcgccgagcg cagacccgag
     6301 agctttacca ctcctgaagg ccctaaaccc cgttccagat gctctgactg ggcaagtgca
     6361 gttgaagaag atgaaatgag gaccagagtt aacaaagaaa tggcaagata taaaaggaaa
     6421 ctcctcatca atgactttgg aagagagaga aaatgatcta gagtacccgg gctaggatcc
     6481 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     6541 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     6601 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     6661 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     6721 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     6781 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     6841 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     6901 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     6961 acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     7021 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat gggcaccacc
     7081 ctggacgaca cggcttaccg ataccgcacc agtgtccctg gggacgccga ggccatcgag
     7141 gcactggatg ggtccttcac caccgacacc gtcttccgcg tcaccgccac cggggacggc
     7201 ttcaccctgc gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa
     7261 tcggacgacg aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg
     7321 tacggggacg acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc
     7381 cggctgaccg tcgaggacat tgaggtagca cccgagcaca ggggacacgg ggtgggacgg
     7441 gcattgatgg gactggctac cgagttcgcc agagaacggg gagctggtca tctttggctc
     7501 gaagtcacca acgtcaacgc accggcgatc cacgcttacc ggcggatggg gttcaccctc
     7561 tgcggcctgg acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac
     7621 atgtccatgc cctgccccgg cagtggcgag ggcagaggaa gtctgctaac atgcggtgac
     7681 gtcgaggaga atcctggccc aatgaagcgc ttcctcttcc tcctactcac catcagcctc
     7741 ctggttatgg tacagataca aactggactc tcaggacaaa acgacaccag ccaaaccagc
     7801 agcccctcag catccagcaa cataagcgga ggcattttcc ttttcttcgt ggctaatgcc
     7861 ataatccacc tcttctgctt cagttgaggc gcgccaaatt tatcgatacc gtcgacctcg
     7921 atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     7981 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     8041 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8101 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     8161 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     8221 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     8281 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     8341 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     8401 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     8461 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8521 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8581 ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca
     8641 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8701 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8761 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8821 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8881 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8941 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9001 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9061 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9121 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9181 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9241 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9301 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9361 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9421 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9481 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9541 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9601 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9661 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9721 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9781 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9841 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9901 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9961 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10021 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10081 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10141 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10201 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10261 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10321 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10381 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
