LOCUS       sequence-437538-        7304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsDcp1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Tritschler F, Braun JE, Motz C, Igreja C, Haas G, Truffault V, 
            Izaurralde E, Weichenrieder O
  TITLE     DCP1 forms asymmetric trimers to assemble into active mRNA decapping
            complexes in metazoa.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6.
  PUBMED    19966221
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Dec 22. 106(51):21591-6."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsDCP1a_G
FEATURES             Location/Qualifiers
     source          1..7304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             24..50
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1847..1968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1858..1877)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1912..1931
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2154..2609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2241..2260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2451..2472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2726..3083
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2726..2917
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2749..2769)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2934..3069
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3134..3928
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3188..3207)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3798..3817
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3992..4040
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4081..4100)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4200..4222
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4260..4278)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4346..4450
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4451..4519,4520..5311)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4669..4688)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5482..6070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5971..5990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6281..6660
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6661..6872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6823..6843
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6869..6893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          7033..7165
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     7148..7167
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        7210..7228
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7232..7241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7238..7303
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
ORIGIN
        1 caccgctcga gaattcaatg gcttacccat acgatgttcc agattacgct atggaggcgc
       61 tgagtcgagc tgggcaggag atgagcctag cggccctgaa gcaacacgac ccctatatca
      121 ccagcatcgc agacctcacg ggccaggtcg ctctgtacac cttctgcccc aaggccaacc
      181 agtgggagaa gactgatata gaagggacct tattcgtata tcgaaggtca gcttcccctt
      241 accatggttt taccattgtg aatcgactaa atatgcacaa tctagtcgaa ccagtgaata
      301 aagatttgga atttcagctc catgaaccat ttcttctgta tagaaatgca agcttgtcga
      361 tatatagtat ctggttttat gacaagaatg actgtcaccg catagcaaaa ctcatggctg
      421 atgtggtaga agaggagaca cggcgatccc agcaagctgc tcgggacaaa cagagtccca
      481 gccaggccaa tggctgcagc gaccacaggc ccatcgacat cctggagatg ctgagcagag
      541 ccaaggatga gtatgagagg aatcagatgg gtgactcaaa tatctccagc cctgggttac
      601 agccaagcac tcagctctcc aatctgggaa gcaccgagac tctagaagaa atgccctccg
      661 ggtcacagga taagtctgct ccatctggac acaagcatct gacggtagaa gagttatttg
      721 gaacctcttt gccaaaggaa caaccagcag ttgtgggtct ggattcagaa gaaatggaga
      781 ggttgccagg agatgcctcc cagaaagagc ccaattcatt cctaccattt ccctttgagc
      841 agttaggagg agcccctcaa tcagaaaccc tgggtgtccc ttctgctgcc caccattcag
      901 tccagcctga aatcaccacc ccggtgctaa tcactccagc ctccatcaca cagtccaatg
      961 aaaagcatgc tccaacctac acaatcccgt tgagccctgt tctcagtccc actctgccag
     1021 ctgaagctcc tactgcacag gttcccccca gcttacctcg aaacagcacc atgatgcagg
     1081 cagtgaagac cacgcctaga cagaggtctc cactcctgaa ccagccagtc cctgagctaa
     1141 gccatgccag tctgattgcc aaccagagcc ccttcagggc cccattgaac gtgacgaaca
     1201 cagctggcac atccctccca agcgttgatc ttctccagaa actcaggttg accccacagc
     1261 atgaccaaat acagacacaa ccacttggga aaggtgcaat ggtagccagc ttttctccgg
     1321 cagctggtca gctagccaca cctgagagct tcatagagcc tccctctaag acagcagcag
     1381 caagagtggc ggcctcagcc tccctgagca acatggtgct tgctcccctt cagtctatgc
     1441 agcagaacca ggatcctgaa gtatttgtgc agcctaaggt gttatccagt gccatcccgg
     1501 ttgcaggcgc cccactggtt actgcaacga ccactgcagt gtcttcagtc ctgctggccc
     1561 caagtgtttt ccagcagaca gttacaagat cttcggacct tgagaggaaa gccagctccc
     1621 cttctcctct aactattgga acgccagaaa gtcagagaaa gccttccatt attctcagca
     1681 agtctcagct ccaggataca ttaatacatc taataaagaa tgattccagc ttcctcagta
     1741 cacttcatga agtctacttg caggtgctga ccaagaacaa agacaaccac aacctatgag
     1801 gcggccgctt ccctttagtg agggttaatg cttcgagcag acatgataag atacattgat
     1861 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     1921 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     1981 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     2041 aacctctaca aatgtggtaa aatccgataa ggatcgatcc gggctggcgt aatagcgaag
     2101 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     2161 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2221 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2281 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2341 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2401 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2461 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2521 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2581 ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     2641 tgcggtattt cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg
     2701 aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     2761 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2821 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2881 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     2941 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3001 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3061 ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa
     3121 ggctagagcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3181 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3241 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3301 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3361 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3421 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3481 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3541 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3601 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     3661 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     3721 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     3781 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     3841 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     3901 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     3961 gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt cattacatct
     4021 gtgtgttggt tttttgtgtg aatcgatagc gataaggatc cgcgtatggt gcactctcag
     4081 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4141 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4201 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4261 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4321 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4381 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4441 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4501 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4561 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4621 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4681 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4741 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4801 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4861 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4921 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4981 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5041 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5101 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5161 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5221 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5281 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5341 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5401 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5461 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5521 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5581 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5641 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5701 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5761 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5821 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5881 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5941 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6001 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6061 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6121 tgctcacatg gctcgacaga tcttcaatat tggccattag ccatattatt cattggttat
     6181 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     6241 tacatttata ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt
     6301 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     6361 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6421 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6481 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6541 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6601 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6661 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     6721 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     6781 tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt gacgcaaatg ggcggtaggc
     6841 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
     6901 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa
     6961 cagtctcgaa cttaagctgc agtgactctc ttaaggtagc cttgcagaag ttggtcgtga
     7021 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
     7081 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
     7141 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
     7201 tagagtactt aatacgactc actataggct agccaccatg gacgcacaaa cacgacgacg
     7261 tgagcgtcgc gctgagaaac aagctcaatg gaaagctgca aacc
//
