LOCUS       sequence-437548-        3710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-nsp6
FEATURES             Location/Qualifiers
     source          1..3710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     444..463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     697..714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(873..900)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(992..1078)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1127..1149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1141..1158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1142..1158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1174..1273
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2194..2293)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2310..2329)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2311..2329)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2334..2350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2334..2350)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2781..3572
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3665..3710,1..543)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
       61 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      721 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      781 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
      841 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
      901 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
      961 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     1021 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     1081 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     1141 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
     1201 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
     1261 aaaaaagcag aagggccgtc aaggccagga tccatgggat cctcaaaacg cactagattg
     1321 tttaaaggca ctgtttgttg gattatggct tctacatttt tgtttagttg cataattaca
     1381 gcatttgtga aatggactat gtttatgtat gtaactacta atatgtttag tattacgttt
     1441 tgtgcacttt gtgttataag tttggccatg ttgttggtta agcataagca tctttatttg
     1501 actatgtata taactcctgt gctttttaca ctgttgtata acaactattt ggttgtgtac
     1561 aagcatacat ttagaggcta tgtctatgca tggctatcat attatgttcc atcagttgag
     1621 tacacttata ctgatgaagt tatttatggc atgttattgc ttgtaggaat ggtctttgtt
     1681 acattacgta gcattaacca tgatttgttt tcttttataa tgtttgttgg tcgtttgatt
     1741 tctgttttct ctttgtggta caagggttct aacttagagg aagaaattct tcttatgttg
     1801 gcttcccttt ttggtactta cacatggaca acagttttat ctatggctgt agcaaaggtt
     1861 attgctaagt gggttgctgt gaatgtcttg tatttcacag atatacctca aattaagata
     1921 gtgcttttgt gctatttgtt tattggttat attattagct gttattgggg cttgttttcc
     1981 ttgatgaaca gtttgtttag aatgcctttg ggtgtttata attataaaat ttcagtacag
     2041 gaattaagat atatgaatgc taatggattg cgccctccta agaatagttt tgaagccctt
     2101 atgcttaatt ttaagctgtt gggtattgga ggtgttccaa tcattgaagt atctcaattt
     2161 caacttaatt aagacgcgat cgcggcctca tgggcccagc tttcttgtac aaagttggca
     2221 ttataaaaaa taattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2281 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2341 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2401 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2461 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2521 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2581 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2641 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2701 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2761 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2821 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2881 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2941 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3001 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3061 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3121 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3181 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3241 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3301 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3361 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3421 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3481 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3541 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3601 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
//
