LOCUS       sequence-437550-        3116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-nsp7
FEATURES             Location/Qualifiers
     source          1..3116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     407..426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     660..677
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(836..863)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(955..1041)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1090..1112
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1104..1121
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1105..1121
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1137..1236
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1563..1662)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1679..1698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1680..1698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1703..1719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1703..1719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2150..2941
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3034..3116,1..506)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
       61 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      121 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      181 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      241 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      301 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      361 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      421 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      481 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      541 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      601 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      661 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      721 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      781 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
      841 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
      901 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
      961 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     1021 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     1081 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa
     1141 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     1201 tttttataat gccaactttg tacaaaaaag cagaagggcc gtcaaggcca ggatccatgg
     1261 gatcctcaaa attgactgat gtcaaatgtg ctaatgtcgt cttgcttaat tgcttgcaac
     1321 atttgcatgt tgcttctaat tctaagttgt ggcattattg tagcactttg cacaatgaaa
     1381 tacttgccac ttcggatctg agtgttgctt ttgaaaagct tgctcagtta ttaattgttt
     1441 tgtttgctaa tccagctgct gtggatagca agtgcctgac tagtattgaa gaagtttgcg
     1501 atgattacgc aaaggacaat actgttttgc agcttaatta agacgcgatc gcggcctcat
     1561 gggcccagct ttcttgtaca aagttggcat tataaaaaat aattgctcat caatttgttg
     1621 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1681 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1741 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     1801 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     1861 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     1921 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1981 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     2041 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2101 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     2161 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2221 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2281 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2341 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2401 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2461 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2521 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2581 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2641 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2701 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2761 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2821 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2881 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2941 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3001 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3061 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatc
//
