LOCUS       sequence-437554-        4118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-Hemagglutinin-esterase
FEATURES             Location/Qualifiers
     source          1..4118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     434..453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     687..704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(863..890)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(982..1068)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1117..1139
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1131..1148
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1132..1148
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1164..1263
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2592..2691)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2708..2727)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2709..2727)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2732..2748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2732..2748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3179..3970
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4063..4118,1..533)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
       61 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      721 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      781 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
      841 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
      901 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
      961 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     1021 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     1081 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
     1141 cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
     1201 cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag
     1261 aagggccgtc aaggccagga tccatgggat cctttttgct tcctagattt attctagtta
     1321 gctgcataat tggtagctta ggtttttaca accctcctac caatgttgtt tcgcatgtaa
     1381 atggagattg gtttttattt ggtgacagtc gttcagattg taatcatatt gttaatatca
     1441 acccccataa ttattcttat atggacctta atcctgttct gtgtgattct ggtaaaatat
     1501 catctaaagc tggcaactcc atttttagga gttttcactt taccgatttt tataattaca
     1561 caggcgaagg tcaacaaatt attttttatg agggtgttaa ttttacgcct tatcatgcct
     1621 ttaaatgcaa ccgttctggt agtaatgata tttggatgca gaataaaggc ttgttttata
     1681 ctcaggttta taagaatatg gctgtgtatc gcagccttac ttttgttaat gtaccatatg
     1741 tttataatgg ctccgcacaa gctacagctc tttgtaaatc tggtagttta gtccttaata
     1801 accctgcata tatagctcct caagctaact ctggggatta ttattataag gttgaagctg
     1861 atttttattt gtcaggttgt gacgagtata tcgtaccact ttgtattttt aacggcaagt
     1921 ttttgtcgaa tacaaagtat tatgatgata gtcaatatta ttttaataaa gacactggtg
     1981 ttatttatgg tctcaattct acagaaacca ttaccactgg ttttgatctt aattgttatt
     2041 atttagtttt accctctggt aattatttag ccatttcaaa tgagctattg ttaactgttc
     2101 ctacgaaagc aatctgtctt aataagcgta aggattttac gcctgtacag gttgttgatt
     2161 cgcggtggaa caatgccagg cagtctgata acatgacggc ggttgcttgt caacctccgt
     2221 actgttattt tcgtaattct actaccaact atgttggtgt ttatgatatt aatcatggag
     2281 atgctggttt tactagcata cttagtggtt tgttatataa ttcaccttgt ttttcgcagc
     2341 aaggcgtttt taggtatgat aatgttagca gtgtctggcc tctctacccc tatggcagat
     2401 gtcccactgc tgctgatatt aatatccctg atttacccat ttgtgtgtat gatccgctac
     2461 cagttatttt gcttggcatt cttttgggcg ttgcgattgt aattattgta gttttgttgt
     2521 tatattttat ggtggataat gttactaggc tgcatgatgc tcttaattaa gacgcgatcg
     2581 cggcctcatg ggcccagctt tcttgtacaa agttggcatt ataaaaaata attgctcatc
     2641 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2701 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2761 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     2821 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     2881 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     2941 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     3001 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     3061 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     3121 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     3181 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     3241 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     3301 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     3361 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     3421 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     3481 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     3541 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     3601 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     3661 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     3721 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     3781 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3841 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3901 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3961 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     4021 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4081 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgc
//
